Identification of miRNAs Involved in Bacillus velezensis FZB42-Activated Induced Systemic Resistance in Maize
Abstract
:1. Introduction
2. Results
2.1. Global miRNA Profile Analysis
2.2. Identification of miRNAs in Maize
2.3. Differentially Expressed miRNAs Responsive to Bacillus
2.4. Target Prediction of Differentially Expressed miRNAs
3. Discussion
4. Methods
4.1. Microbial Strains, Plant and Growth Conditions
4.2. Construction and Analysis of Small RNA Libraries
4.3. Identification of Induced Systemic Resistance-Associated miRNAs
4.4. Analysis of miRNA Promoter
4.5. cDNA Library Construction for Degradome Sequencing
4.6. Prediction of miRNA Targets
4.7. qRT-PCR Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Xie, S.; Yu, H.; Li, E.; Wang, Y.; Liu, J.; Jiang, H. Identification of miRNAs Involved in Bacillus velezensis FZB42-Activated Induced Systemic Resistance in Maize. Int. J. Mol. Sci. 2019, 20, 5057. https://doi.org/10.3390/ijms20205057
Xie S, Yu H, Li E, Wang Y, Liu J, Jiang H. Identification of miRNAs Involved in Bacillus velezensis FZB42-Activated Induced Systemic Resistance in Maize. International Journal of Molecular Sciences. 2019; 20(20):5057. https://doi.org/10.3390/ijms20205057
Chicago/Turabian StyleXie, Shanshan, Hengguo Yu, Enze Li, Yu Wang, Juan Liu, and Haiyang Jiang. 2019. "Identification of miRNAs Involved in Bacillus velezensis FZB42-Activated Induced Systemic Resistance in Maize" International Journal of Molecular Sciences 20, no. 20: 5057. https://doi.org/10.3390/ijms20205057