Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin
Abstract
:1. Introduction
2. Results
2.1. Effect of ZnSO4 Injection on the RBC Level of 5/6-Nephrectomized Rats
2.2. New Methylene Blue Staining of the Blood Cells of 5/6-Nephrectomized Rats Injected with Either Saline or ZnSO4
2.3. Effect of ZnSO4 Injection on the EPO Level in the 5/6-Nephrectomized Rats
2.4. In Vivo Comparison of the Injection of Saline, ZnSO4, or rHuEPO on the RBC Levels of 5/6-Nephrectomized and Normal Rats
2.5. Effect of ZnSO4 or rHuEPO Injection on Blood Urea Nitrogen and Creatinine of Rats
2.6. In Vitro Comparison of Zinc- and rHuEPO-Induced Erythropoiesis in Rat Bone Marrow Cells
2.7. Effect of ZnCl2 Supplementation on the Growth of 5.1-μm Cells (Immature Reticulocytes) and Production of EPO from Rat Bone Marrow Cells
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Effect of ZnSO4 Injection on the RBC Level of 5/6-Nephretomized Rats
4.3. Microscopic Observation of Rat Blood Cells Using New Methylene Blue Staining
4.4. Comparison between the Injection of Saline, ZnSO4, or rHuEPO on the RBC Level of 5/6-Nephrectomized Rats
4.5. Determination of Blood Urea Nitrogen and Creatinine Contents
4.6. Comparison of Cell Growth Induced Using ZnCl2 or rHuEPO Supplementation in Rat Bone Marrow Cells
4.7. Effect of ZnCl2 Supplementation on the Growth of 5.1-μm Cells (Immature Reticulocytes) and Production of EPO From Rat Bone Marrow Cells
4.8. Determination of the EPO Concentration
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CKD | chronic kidney disease |
EPO | erythropoietin |
rHuEPO | recombinant human erythropoietin |
RBC | red blood cell |
PHZ | phenylhydrazine |
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Feng, H.-L.; Chen, Y.-H.; Jeng, S.-S. Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin. Int. J. Mol. Sci. 2019, 20, 4985. https://doi.org/10.3390/ijms20204985
Feng H-L, Chen Y-H, Jeng S-S. Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin. International Journal of Molecular Sciences. 2019; 20(20):4985. https://doi.org/10.3390/ijms20204985
Chicago/Turabian StyleFeng, Hui-Lin, Yen-Hua Chen, and Sen-Shyong Jeng. 2019. "Effect of Zinc Supplementation on Renal Anemia in 5/6-Nephrectomized Rats and a Comparison with Treatment with Recombinant Human Erythropoietin" International Journal of Molecular Sciences 20, no. 20: 4985. https://doi.org/10.3390/ijms20204985