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Open AccessArticle

Methionine Adenosyltransferase 1a (MAT1A) Enhances Cell Survival During Chemotherapy Treatment and is Associated with Drug Resistance in Bladder Cancer PDX Mice

1
Lawrence Livermore National Laboratory, Physical and Life Sciences Directorate, Livermore, CA 94550, USA
2
Department of Biochemistry & Molecular Biology, Georgetown University, Washington, DC 20057, USA
3
UC Merced, School of Natural Sciences, Merced, CA 95343, USA
4
UC Davis Comprehensive Cancer Center, Sacramento, CA 95817, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 4983; https://doi.org/10.3390/ijms20204983
Received: 14 September 2019 / Revised: 30 September 2019 / Accepted: 3 October 2019 / Published: 9 October 2019
(This article belongs to the Special Issue Cancer Cell Metabolism—Pathways and Biology)
Bladder cancer is among the top ten most common cancers, with about ~380,000 new cases and ~150,000 deaths per year worldwide. Tumor relapse following chemotherapy treatment has long been a significant challenge towards completely curing cancer. We have utilized a patient-derived bladder cancer xenograft (PDX) platform to characterize molecular mechanisms that contribute to relapse following drug treatment in advanced bladder cancer. Transcriptomic profiling of bladder cancer xenograft tumors by RNA-sequencing analysis, before and after relapse, following a 21-day cisplatin/gemcitabine drug treatment regimen identified methionine adenosyltransferase 1a (MAT1A) as one of the significantly upregulated genes following drug treatment. Survey of patient tumor sections confirmed elevated levels of MAT1A in individuals who received chemotherapy. Overexpression of MAT1A in 5637 bladder cancer cells increased tolerance to gemcitabine and stalled cell proliferation rates, suggesting MAT1A upregulation as a potential mechanism by which bladder cancer cells persist in a quiescent state to evade chemotherapy. View Full-Text
Keywords: bladder cancer; drug resistance; methyltransferase; gemcitabine bladder cancer; drug resistance; methyltransferase; gemcitabine
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Martin, K.A.; Hum, N.R.; Sebastian, A.; He, W.; Siddiqui, S.; Ghosh, P.M.; Pan, C.-X.; de Vere White, R.; Loots, G.G. Methionine Adenosyltransferase 1a (MAT1A) Enhances Cell Survival During Chemotherapy Treatment and is Associated with Drug Resistance in Bladder Cancer PDX Mice. Int. J. Mol. Sci. 2019, 20, 4983.

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