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Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes

1
College of Biological Science, Jining Medical University, Ri’zhao 276800, Shandong, China
2
College of Life Science, Nanjing University, Nan’jing 210046, Jiangshu, China
3
College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, Shandong, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(2), 367; https://doi.org/10.3390/ijms20020367
Received: 18 December 2018 / Revised: 9 January 2019 / Accepted: 13 January 2019 / Published: 16 January 2019
(This article belongs to the Special Issue Molecular Chaperones 2.0)
DnaJ proteins, which are molecular chaperones that are widely present in plants, can respond to various environmental stresses. At present, the function of DnaJ proteins was studied in many plant species, but only a few studies were conducted in tomato. Here, we examined the functions of a novel tomato (Solanum lycopersicum) DnaJ protein (SlDnaJ20) in heat tolerance using sense and antisense transgenic tomatoes. Transient conversion assays of Arabidopsis protoplasts showed that SlDnaJ20 was targeted to chloroplasts. Expression analysis showed that SlDnaJ20 expression was induced by chilling, NaCl, polyethylene glycol, and H2O2, especially via heat stress. Under heat stress, sense plants showed higher fresh weights, chlorophyll content, fluorescence (Fv/Fm), and D1 protein levels, and a lower accumulation of reactive oxygen species (ROS) than antisense plants. These results suggest that SlDnaJ20 overexpression can reduce the photoinhibition of photosystem II (PSII) by relieving ROS accumulation. Moreover, higher expression levels of HsfA1 and HsfB1 were observed under heat stress in sense plants, indicating that SlDnaJ20 overexpression contributes to HSF expression. The yeast two-hybrid system proved that SlDnaJ20 can interact with the chloroplast heat-shock protein 70. Our results indicate that SlDnaJ20 overexpression enhances the thermotolerance of transgenic tomatoes, whereas suppression of SlDnaJ20 increases the heat sensitivity of transgenic tomatoes. View Full-Text
Keywords: DnaJ; heat stress; HSP70; SlDnaJ20; tomato DnaJ; heat stress; HSP70; SlDnaJ20; tomato
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MDPI and ACS Style

Wang, G.; Cai, G.; Xu, N.; Zhang, L.; Sun, X.; Guan, J.; Meng, Q. Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes. Int. J. Mol. Sci. 2019, 20, 367. https://doi.org/10.3390/ijms20020367

AMA Style

Wang G, Cai G, Xu N, Zhang L, Sun X, Guan J, Meng Q. Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes. International Journal of Molecular Sciences. 2019; 20(2):367. https://doi.org/10.3390/ijms20020367

Chicago/Turabian Style

Wang, Guodong; Cai, Guohua; Xu, Na; Zhang, Litao; Sun, Xiuling; Guan, Jing; Meng, Qingwei. 2019. "Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes" Int. J. Mol. Sci. 20, no. 2: 367. https://doi.org/10.3390/ijms20020367

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