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Open AccessArticle

Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12

Department of Oral Maxillofacial Surgery, Division of Regenerative Orofacial Medicine, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
Department of Oral Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf; 20246 Hamburg, Germany
Institute of Materials Research, Division Metallic Biomaterials, Helmholtz-Zentrum Geesthacht, 21502 Geesthacht, Germany
Department of Materials Test Engineering (WPT), TU Dortmund University, 44227 Dortmund, Germany
Institute of Materials Engineering, TU Dortmund University, 44227 Dortmund, Germany
Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Oral and Maxillofacial Surgery, 12200 Berlin, Germany
Berlin Institute of Health, 10178 Berlin, Germany
BerlinAnalytix GmbH, 12109 Berlin, Germany
Meotec GmbH & Co. KG, 52068 Aachen, Germany
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(2), 255;
Received: 27 November 2018 / Revised: 22 December 2018 / Accepted: 30 December 2018 / Published: 10 January 2019
(This article belongs to the Special Issue Biomaterials for Bone Tissue Engineering)
Magnesium (Mg)-based biomaterials are promising candidates for bone and tissue regeneration. Alloying and surface modifications provide effective strategies for optimizing and tailoring their degradation kinetics. Nevertheless, biocompatibility analyses of Mg-based materials are challenging due to its special degradation mechanism with continuous hydrogen release. In this context, the hydrogen release and the related (micro-) milieu conditions pretend to strictly follow in vitro standards based on ISO 10993-5/-12. Thus, special adaptions for the testing of Mg materials are necessary, which have been described in a previous study from our group. Based on these adaptions, further developments of a test procedure allowing rapid and effective in vitro cytocompatibility analyses of Mg-based materials based on ISO 10993-5/-12 are necessary. The following study introduces a new two-step test scheme for rapid and effective testing of Mg. Specimens with different surface characteristics were produced by means of plasma electrolytic oxidation (PEO) using silicate-based and phosphate-based electrolytes. The test samples were evaluated for corrosion behavior, cytocompatibility and their mechanical and osteogenic properties. Thereby, two PEO ceramics could be identified for further in vivo evaluations. View Full-Text
Keywords: magnesium; ISO10993-5/-12; PEO; degradation; biocompatibility; implant magnesium; ISO10993-5/-12; PEO; degradation; biocompatibility; implant
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Jung, O.; Smeets, R.; Hartjen, P.; Schnettler, R.; Feyerabend, F.; Klein, M.; Wegner, N.; Walther, F.; Stangier, D.; Henningsen, A.; Rendenbach, C.; Heiland, M.; Barbeck, M.; Kopp, A. Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12. Int. J. Mol. Sci. 2019, 20, 255.

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