Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model
Abstract
:1. Introduction
2. Results
2.1. Low-Energy SW Significantly Reduced Blood Glucose, Hemoglobin A1c and Urine Volume in DM
2.2. Low-Energy SW Significantly Enhanced Pancreatic Islets Area, c-Peptide, GLP-1 and Insulin Production
2.3. Low-Energy SW Enhanced Beta Cells Regeneration
2.4. Low-Energy SW Enhanced Tissue Repair and Angiogenesis
2.5. Low-Energy SW Alleviated Oxidative Stress and Cell Apoptosis
2.6. Low-Energy SW Prevented Diabetes-Induced Pancreatic Tissue Inflammation
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Establishment of the DM Rat Model
4.3. Low-Energy SW Treatment.
4.4. Measurement of Urine Volume and 8-Hydroxy-2’-Deoxyguanosine (8-OHdG)
4.5. Hematoxylin and Eosin (HE) Stain, Immunohistochemistry, and Immunofluorescence
4.6. Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL)
4.7. Western Blotting
4.8. Enzyme-Linked Immunosorbent Assay (ELISA) Analysis
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
DM | streptozotocin induced diabetes |
SW | extracorporeal shock wave |
HbA1c | hemoglobin A1c |
GLP-1 | glucagon-like peptide 1 |
SDF-1 | stromal cell derived factor 1 |
IL-1β | interleukin-1β |
TNFα | tumor necrosis factor-α |
Normal | normal control |
shockwave | low-energy SW |
HE | hematoxylin and eosin |
IHC | immunohistochemistry |
IF | immunofluorescence |
PCNA | proliferating cell nuclear antigen |
EPCs | endothelial progenitor cells |
vWF | von Willebrand factor |
VEGF | vascular endothelial growth factor |
8-OHdG | 8-hydroxy-2’ -deoxyguanosine |
TUNEL | terminal deoxynucleotidyl transferase dUTP nick end labeling |
STZ | streptozotocin |
ELISA | enzyme-linked immunosorbent assay |
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Hsiao, C.-C.; Lin, C.-C.; Hou, Y.-S.; Ko, J.-Y.; Wang, C.-J. Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model. Int. J. Mol. Sci. 2019, 20, 4934. https://doi.org/10.3390/ijms20194934
Hsiao C-C, Lin C-C, Hou Y-S, Ko J-Y, Wang C-J. Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model. International Journal of Molecular Sciences. 2019; 20(19):4934. https://doi.org/10.3390/ijms20194934
Chicago/Turabian StyleHsiao, Chang-Chun, Cheng-Chan Lin, You-Syuan Hou, Jih-Yang Ko, and Ching-Jen Wang. 2019. "Low-Energy Extracorporeal Shock Wave Ameliorates Streptozotocin Induced Diabetes and Promotes Pancreatic Beta Cells Regeneration in a Rat Model" International Journal of Molecular Sciences 20, no. 19: 4934. https://doi.org/10.3390/ijms20194934