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Open AccessArticle

In Vivo Simulation of Magnesium Degradability Using a New Fluid Dynamic Bench Testing Approach

1
Department of Oral Maxillofacial Surgery, University Medical Center, 20246 Hamburg-Eppendorf, Germany
2
Department of Materials Test Engineering (WPT), TU Dortmund University, 44227 Dortmund, Germany
3
Meotec GmbH, 52068 Aachen, Germany
4
Institute of Materials Research, Division Metallic Biomaterials, Helmholtz-Zentrum Geesthacht, 21502 Geesthacht, Germany
5
BerlinAnalytix GmbH, 12109 Berlin, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(19), 4859; https://doi.org/10.3390/ijms20194859
Received: 10 August 2019 / Revised: 21 September 2019 / Accepted: 23 September 2019 / Published: 30 September 2019
(This article belongs to the Special Issue Biomaterials for Soft and Hard Tissue Regeneration)
The degradation rate of magnesium (Mg) alloys is a key parameter to develop Mg-based biomaterials and ensure in vivo-mechanical stability as well as to minimize hydrogen gas production, which otherwise can lead to adverse effects in clinical applications. However, in vitro and in vivo results of the same material often differ largely. In the present study, a dynamic test bench with several single bioreactor cells was constructed to measure the volume of hydrogen gas which evolves during magnesium degradation to indicate the degradation rate in vivo. Degradation medium comparable with human blood plasma was used to simulate body fluids. The media was pumped through the different bioreactor cells under a constant flow rate and 37 °C to simulate physiological conditions. A total of three different Mg groups were successively tested: Mg WE43, and two different WE43 plasma electrolytically oxidized (PEO) variants. The results were compared with other methods to detect magnesium degradation (pH, potentiodynamic polarization (PDP), cytocompatibility, SEM (scanning electron microscopy)). The non-ceramized specimens showed the highest degradation rates and vast standard deviations. In contrast, the two PEO samples demonstrated reduced degradation rates with diminished standard deviation. The pH values showed above-average constant levels between 7.4–7.7, likely due to the constant exchange of the fluids. SEM revealed severe cracks on the surface of WE43 after degradation, whereas the ceramized surfaces showed significantly decreased signs of corrosion. PDP results confirmed the improved corrosion resistance of both PEO samples. While WE43 showed slight toxicity in vitro, satisfactory cytocompatibility was achieved for the PEO test samples. In summary, the dynamic test bench constructed in this study enables reliable and simple measurement of Mg degradation to simulate the in vivo environment. Furthermore, PEO treatment of magnesium is a promising method to adjust magnesium degradation. View Full-Text
Keywords: bioreactor; magnesium; degradation; plasma electrolytic oxidation (PEO) bioreactor; magnesium; degradation; plasma electrolytic oxidation (PEO)
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Jung, O.; Porchetta, D.; Schroeder, M.-L.; Klein, M.; Wegner, N.; Walther, F.; Feyerabend, F.; Barbeck, M.; Kopp, A. In Vivo Simulation of Magnesium Degradability Using a New Fluid Dynamic Bench Testing Approach. Int. J. Mol. Sci. 2019, 20, 4859.

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