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Open AccessArticle

Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves

1
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 230 53 Alnarp, Sweden
2
Department of Immunotechnology, Lund University, 221 00 Lund, Sweden
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(19), 4726; https://doi.org/10.3390/ijms20194726
Received: 3 July 2019 / Revised: 16 September 2019 / Accepted: 22 September 2019 / Published: 24 September 2019
(This article belongs to the Special Issue Plant Innate Immunity 3.0)
Plants have a variety of ways to defend themselves against pathogens. A commonly used model of the plant immune system is divided into a general response triggered by pathogen-associated molecular patterns (PAMPs), and a specific response triggered by effectors. The first type of response is known as PAMP triggered immunity (PTI), and the second is known as effector-triggered immunity (ETI). To obtain better insight into changes of protein abundance in immunity reactions, we performed a comparative proteomic analysis of a PTI and two different ETI models (relating to Phytophthora infestans) in potato. Several proteins showed higher abundance in all immune reactions, such as a protein annotated as sterol carrier protein 2 that could be interesting since Phytophthora species are sterol auxotrophs. RNA binding proteins also showed altered abundance in the different immune reactions. Furthermore, we identified some PTI-specific changes of protein abundance, such as for example, a glyoxysomal fatty acid beta-oxidation multifunctional protein and a MAR-binding protein. Interestingly, a lysine histone demethylase was decreased in PTI, and that prompted us to also analyze protein methylation in our datasets. The proteins upregulated explicitly in ETI included several catalases. Few proteins were regulated in only one of the ETI interactions. For example, histones were only downregulated in the ETI-Avr2 interaction, and a putative multiprotein bridging factor was only upregulated in the ETI-IpiO interaction. One example of a methylated protein that increased in the ETI interactions was a serine hydroxymethyltransferase. View Full-Text
Keywords: potato; immunity; proteomics; methylation; plants; ETI; PTI; solanum; late blight potato; immunity; proteomics; methylation; plants; ETI; PTI; solanum; late blight
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MDPI and ACS Style

Resjö, S.; Zahid, M.A.; Burra, D.D.; Lenman, M.; Levander, F.; Andreasson, E. Proteomics of PTI and Two ETI Immune Reactions in Potato Leaves. Int. J. Mol. Sci. 2019, 20, 4726.

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