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Article

Rapid or Slow Time to Brain Death? Impact on Kidney Graft Injuries in an Allotransplantation Porcine Model

1
CHU Poitiers, Coordination des prélèvements d’organe et de tissus, F-86000 Poitiers, France
2
CHU Poitiers, Département d’anesthésie-réanimation, F-86000 Poitiers, France
3
INSERM U1082 (IRTOMIT), F-86000 Poitiers, France
4
CHU Poitiers, Service de Biochimie, F-86000 Poitiers, France
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Faculté de Médecine et Pharmacie, Université de Poitiers, F-86000 Poitiers, France
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CHU de Poitiers, Service de Chirurgie viscérale, F-86000 Poitiers, France
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IBiSA ‘plate-forme MOdélisation Préclinique - Innovations Chirurgicale et Technologique (MOPICT)’, Domaine Expérimental du Magneraud, F-17700 Surgères, France
8
INRA, Unité expérimentale Génétique, expérimentations et systèmes innovants (GENESI), Domaine Expérimental du Magneraud, F-17700 Surgères, France
9
CHU Poitiers, Service d’accueil des urgences, SAMU-SMUR, F-86000 Poitiers, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(15), 3671; https://doi.org/10.3390/ijms20153671
Received: 5 July 2019 / Revised: 18 July 2019 / Accepted: 24 July 2019 / Published: 26 July 2019
The use of donors deceased after brain death (DBD) with extended criteria in response to the shortage of grafts leads to the removal of more fragile kidneys. These grafts are at greater risk of not being grafted or delayed function. A better knowledge of the pathophysiology of DBDs would improve this situation. There is a difference between the results from animal models of DBD and the clinical data potentially explained by the kinetics of brain death induction. We compared the effect of the induction rate of brain death on the recovery of post-transplant renal function in a pig model of DBD followed by allografts in nephrectomized pigs. Resumption of early function post-transplant was better in the rapidly generated brain death group (RgBD) and graft fibrosis at three months less important. Two groups had identical oxidative stress intensity but a greater response to this oxidative stress by SIRT1, PGC1-α and NRF2 in the RgBD group. Modulation of mechanistic target of rapamycin (mTOR) stimulation by NRF2 would also regulate the survival/apoptosis balance of renal cells. For the first time we have shown that an allostatic response to oxidative stress can explain the impact of the rapidity of brain death induction on the quality of kidney transplants. View Full-Text
Keywords: brain death; kidney; transplantation; oxidative stress; allostasis; nuclear factor erythroid-2-related factor 2; mechanistic target of rapamycin brain death; kidney; transplantation; oxidative stress; allostasis; nuclear factor erythroid-2-related factor 2; mechanistic target of rapamycin
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MDPI and ACS Style

Kerforne, T.; Giraud, S.; Danion, J.; Thuillier, R.; Couturier, P.; Hebrard, W.; Mimoz, O.; Hauet, T. Rapid or Slow Time to Brain Death? Impact on Kidney Graft Injuries in an Allotransplantation Porcine Model. Int. J. Mol. Sci. 2019, 20, 3671. https://doi.org/10.3390/ijms20153671

AMA Style

Kerforne T, Giraud S, Danion J, Thuillier R, Couturier P, Hebrard W, Mimoz O, Hauet T. Rapid or Slow Time to Brain Death? Impact on Kidney Graft Injuries in an Allotransplantation Porcine Model. International Journal of Molecular Sciences. 2019; 20(15):3671. https://doi.org/10.3390/ijms20153671

Chicago/Turabian Style

Kerforne, Thomas, Sébastien Giraud, Jérôme Danion, Raphael Thuillier, Pierre Couturier, William Hebrard, Olivier Mimoz, and Thierry Hauet. 2019. "Rapid or Slow Time to Brain Death? Impact on Kidney Graft Injuries in an Allotransplantation Porcine Model" International Journal of Molecular Sciences 20, no. 15: 3671. https://doi.org/10.3390/ijms20153671

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