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Article

Identification of a 119-bp Promoter of the Maize Sulfite Oxidase Gene (ZmSO) That Confers High-Level Gene Expression and ABA or Drought Inducibility in Transgenic Plants

1
College of Life Science, Henan Agricultural University, Zhengzhou 450002, China
2
Library of Henan Agricultural University, Zhengzhou 450002, China
3
School of Life Sciences, Henan University, Kaifeng 475004, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(13), 3326; https://doi.org/10.3390/ijms20133326
Received: 13 June 2019 / Revised: 1 July 2019 / Accepted: 3 July 2019 / Published: 6 July 2019
(This article belongs to the Section Molecular Plant Sciences)
Drought adversely affects crop growth and yields. The cloning and characterization of drought- or abscisic acid (ABA)-inducible promoters is of great significance for their utilization in the genetic improvement of crop resistance. Our previous studies have shown that maize sulfite oxidase (SO) has a sulfite-oxidizing function and is involved in the drought stress response. However, the promoter of the maize SO gene has not yet been characterized. In this study, the promoter (ZmSOPro, 1194 bp upstream region of the translation initiation site) was isolated from the maize genome. The in-silico analysis of the ZmSOPro promoter identified several cis-elements responsive to the phytohormone ABA and drought stress such as ABA-responsive element (ABRE) and MYB binding site (MBS), besides a number of core cis-acting elements, such as TATA-box and CAAT-box. A 5′ RACE (rapid amplification of cDNA ends) assay identified an adenine residue as the transcription start site of the ZmSO. The ZmSOPro activity was detected by β-glucuronidase (GUS) staining at nearly all developmental stages and in most plant organs, except for the roots in transgenic Arabidopsis. Moreover, its activity was significantly induced by ABA and drought stress. The 5′-deletion mutant analysis of the ZmSOPro in tobacco plants revealed that a 119-bp fragment in the ZmSOPro (upstream of the transcription start site) is a minimal region, which is required for its high-level expression. Moreover, the minimal ZmSOPro was significantly activated by ABA or drought stress in transgenic plants. Further mutant analysis indicated that the MBS element in the minimal ZmSOPro region (119 bp upstream of the transcription start site) is responsible for ABA and drought-stress induced expression. These results improve our understanding of the transcriptional regulation mechanism of the ZmSO gene, and the characterized 119-bp promoter fragment could be an ideal candidate for drought-tolerant gene engineering in both monocot and dicot crops. View Full-Text
Keywords: maize; sulfite oxidase; promoter; drought; transcriptional regulation maize; sulfite oxidase; promoter; drought; transcriptional regulation
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MDPI and ACS Style

Xu, Z.; Wang, M.; Guo, Z.; Zhu, X.; Xia, Z. Identification of a 119-bp Promoter of the Maize Sulfite Oxidase Gene (ZmSO) That Confers High-Level Gene Expression and ABA or Drought Inducibility in Transgenic Plants. Int. J. Mol. Sci. 2019, 20, 3326. https://doi.org/10.3390/ijms20133326

AMA Style

Xu Z, Wang M, Guo Z, Zhu X, Xia Z. Identification of a 119-bp Promoter of the Maize Sulfite Oxidase Gene (ZmSO) That Confers High-Level Gene Expression and ABA or Drought Inducibility in Transgenic Plants. International Journal of Molecular Sciences. 2019; 20(13):3326. https://doi.org/10.3390/ijms20133326

Chicago/Turabian Style

Xu, Ziwei, Meiping Wang, Ziting Guo, Xianfeng Zhu, and Zongliang Xia. 2019. "Identification of a 119-bp Promoter of the Maize Sulfite Oxidase Gene (ZmSO) That Confers High-Level Gene Expression and ABA or Drought Inducibility in Transgenic Plants" International Journal of Molecular Sciences 20, no. 13: 3326. https://doi.org/10.3390/ijms20133326

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