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Open AccessArticle

CopA Protects Streptococcus suis against Copper Toxicity

by Chengkun Zheng 1,2,3,*, Mengdie Jia 1,2, Tianyu Lu 1,2, Miaomiao Gao 1,2 and Lingzhi Li 1,2
1
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, China
2
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China
3
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(12), 2969; https://doi.org/10.3390/ijms20122969
Received: 1 May 2019 / Revised: 15 June 2019 / Accepted: 16 June 2019 / Published: 18 June 2019
(This article belongs to the Section Molecular Microbiology)
Streptococcus suis is a zoonotic pathogen that causes great economic losses to the swine industry and severe threats to public health. A better understanding of its physiology would contribute to the control of its infections. Although copper is an essential micronutrient for life, it is toxic to cells when present in excessive amounts. Herein, we provide evidence that CopA is required for S. suis resistance to copper toxicity. Quantitative PCR analysis showed that copA expression was specifically induced by copper. Growth curve analyses and spot dilution assays showed that the ΔcopA mutant was defective in media supplemented with elevated concentrations of copper. Spot dilution assays also revealed that CopA protected S. suis against the copper-induced bactericidal effect. Using inductively coupled plasma-optical emission spectroscopy, we demonstrated that the role of CopA in copper resistance was mediated by copper efflux. Collectively, our data indicated that CopA protects S. suis against the copper-induced bactericidal effect via copper efflux. View Full-Text
Keywords: CopA; Streptococcus suis; copper toxicity; copper resistance CopA; Streptococcus suis; copper toxicity; copper resistance
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MDPI and ACS Style

Zheng, C.; Jia, M.; Lu, T.; Gao, M.; Li, L. CopA Protects Streptococcus suis against Copper Toxicity. Int. J. Mol. Sci. 2019, 20, 2969.

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