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In Vitro Study of the Anticancer Effects of Biotechnological Extracts of the Endangered Plant Species Satureja Khuzistanica

1
Secció de Fisiologia i Biotecnologia Vegetal, Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Joan XXIII 27-31, 08028 Barcelona, Spain
2
Secció de Bioquímica i Biologia Molecular, Departament de Bioquímica i Fisiologia, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Joan XXIII 27-31, 08028 Barcelona, Spain
3
Institute of Chemistry and Biotechnology, Biochemical Engineering and Cell Cultivation Techniques, Campus Grüental, Zurich University of Applied Sciences, 8820 Wädenswill, Switzerland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(10), 2400; https://doi.org/10.3390/ijms20102400
Received: 25 April 2019 / Revised: 9 May 2019 / Accepted: 13 May 2019 / Published: 15 May 2019
(This article belongs to the Special Issue Health Promoting Effects of Phytochemicals)
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Abstract

Many medicinal plant species are currently threatened in their natural habitats because of the growing demand for phytochemicals worldwide. A sustainable alternative for the production of bioactive plant compounds are plant biofactories based on cell cultures and organs. In addition, plant extracts from biofactories have significant advantages over those obtained from plants, since they are free of contamination by microorganisms, herbicides and pesticides, and they provide more stable levels of active ingredients. In this context, we report the establishment of Satureja khuzistanica cell cultures able to produce high amounts of rosmarinic acid (RA). The production of this phytopharmaceutical was increased when the cultures were elicited with coronatine and scaled up to a benchtop bioreactor. S. khuzistanica extracts enriched in RA were found to reduce the viability of cancer cell lines, increasing the sub-G0/G1 cell population and the activity of caspase-8 in MCF-7 cells, which suggest that S. khuzistanica extracts can induce apoptosis of MCF-7 cells through activation of the extrinsic pathway. In addition, our findings indicate that other compounds in S. khuzistanica extracts may act synergistically to potentiate the anticancer activity of RA. View Full-Text
Keywords: Satureja khuzistanica; rosmarinic acid; plant cell cultures; coronatine; MCF-7 human breast adenocarcinoma cells; HepG2 human hepatoma cells Satureja khuzistanica; rosmarinic acid; plant cell cultures; coronatine; MCF-7 human breast adenocarcinoma cells; HepG2 human hepatoma cells
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Khojasteh, A.; Metón, I.; Camino, S.; Cusido, R.M.; Eibl, R.; Palazon, J. In Vitro Study of the Anticancer Effects of Biotechnological Extracts of the Endangered Plant Species Satureja Khuzistanica. Int. J. Mol. Sci. 2019, 20, 2400.

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