Proteomic Analysis of Mouse Brain Subjected to Spaceflight

Mao, Xiao Wen; Sandberg, Lawrence B.; Gridley, Daila S.; Herrmann, E. Clifford; Zhang, Guangyu; Raghavan, Ravi; Zubarev, Roman A.; Zhang, Bo; Stodieck, Louis S.; Ferguson, Virginia L.; Bateman, Ted A.; Pecaut, Michael J.

doi:10.3390/ijms20010007

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

Proteomic Analysis of Mouse Brain Subjected to Spaceflight

²,

¹,

²,

²,

³,

^4,5,

^4,5

⁶

⁶,

⁷ and

^1,*

Department of Basic Sciences, Division of Biomedical Engineering Sciences, Loma Linda University School of Medicine, Loma Linda, CA 92354, USA

Department of Biochemistry, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA

Department of Pathology and Human Anatomy, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA

⁴

Department of Medical Biochemistry and Biophysics, Biomedicum, Karolinska Institutet, SE 17177 Stockholm, Sweden

⁵

Department of Pharmacological and Technological Chemistry, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia

⁶

BioServe Space Technologies, University of Colorado at Boulder, Boulder, CO 80303, USA

⁷

Department of Bioengineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(1), 7; https://doi.org/10.3390/ijms20010007

Received: 9 November 2018 / Revised: 11 December 2018 / Accepted: 17 December 2018 / Published: 20 December 2018

(This article belongs to the Special Issue Oxidative Stress and Damage in the Space Environment: Physiological Ground and Flight Parallels)

View Full-Text Download PDF Review Reports Citation Export

Abstract

There is evidence that spaceflight poses acute and late risks to the central nervous system. To explore possible mechanisms, the proteomic changes following spaceflight in mouse brain were characterized. Space Shuttle Atlantis (STS-135) was launched from the Kennedy Space Center (KSC) on a 13-day mission. Within 3–5 h after landing, brain tissue was collected to evaluate protein expression profiles using quantitative proteomic analysis. Our results showed that there were 26 proteins that were significantly altered after spaceflight in the gray and/or white matter. While there was no overlap between the white and gray matter in terms of individual proteins, there was overlap in terms of function, synaptic plasticity, vesical activity, protein/organelle transport, and metabolism. Our data demonstrate that exposure to the spaceflight environment induces significant changes in protein expression related to neuronal structure and metabolic function. This might lead to a significant impact on brain structural and functional integrity that could affect the outcome of space missions. View Full-Text

Keywords: brain; spaceflight; microgravity; proteomics

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Share and Cite

MDPI and ACS Style

Mao, X.W.; Sandberg, L.B.; Gridley, D.S.; Herrmann, E.C.; Zhang, G.; Raghavan, R.; Zubarev, R.A.; Zhang, B.; Stodieck, L.S.; Ferguson, V.L.; Bateman, T.A.; Pecaut, M.J. Proteomic Analysis of Mouse Brain Subjected to Spaceflight. Int. J. Mol. Sci. 2019, 20, 7. https://doi.org/10.3390/ijms20010007

AMA Style

Mao XW, Sandberg LB, Gridley DS, Herrmann EC, Zhang G, Raghavan R, Zubarev RA, Zhang B, Stodieck LS, Ferguson VL, Bateman TA, Pecaut MJ. Proteomic Analysis of Mouse Brain Subjected to Spaceflight. International Journal of Molecular Sciences. 2019; 20(1):7. https://doi.org/10.3390/ijms20010007

Chicago/Turabian Style

Mao, Xiao W., Lawrence B. Sandberg, Daila S. Gridley, E. C. Herrmann, Guangyu Zhang, Ravi Raghavan, Roman A. Zubarev, Bo Zhang, Louis S. Stodieck, Virginia L. Ferguson, Ted A. Bateman, and Michael J. Pecaut. 2019. "Proteomic Analysis of Mouse Brain Subjected to Spaceflight" International Journal of Molecular Sciences 20, no. 1: 7. https://doi.org/10.3390/ijms20010007

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

Proteomic Analysis of Mouse Brain Subjected to Spaceflight

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI