Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury
Abstract
:1. Introduction
2. Results
2.1. Sub-Acute Transplantation of Schwann Cells Intraspinally Reduces Iba1 and CD68 Cells within the Lesion
2.2. SC Transplantation Alters Innate Immune Cell Phenotypes after SCI
2.3. Macrophages and Microglia within SC Transplants Exhibited a Dramatic Change in Cell Morphology and Were Found in a Parallel Association with SCs and Axons
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Schwann Cell Culture
4.3. Introduction of Lentiviral Vectors into SCs
4.4. Animals
4.5. Pre-Operative Preparation
4.6. Moderate Thoracic Spinal Cord Contusion Injury
4.7. Intraspinal Implantation of Schwann Cells
4.8. Post-Operative Care
4.9. Flow Cytometry
4.10. Histology and Immunocytochemistry
4.11. Image Analysis
4.12. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Pearse, D.D.; Bastidas, J.; Izabel, S.S.; Ghosh, M. Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury. Int. J. Mol. Sci. 2018, 19, 2550. https://doi.org/10.3390/ijms19092550
Pearse DD, Bastidas J, Izabel SS, Ghosh M. Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury. International Journal of Molecular Sciences. 2018; 19(9):2550. https://doi.org/10.3390/ijms19092550
Chicago/Turabian StylePearse, Damien D., Johana Bastidas, Sarah S. Izabel, and Mousumi Ghosh. 2018. "Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury" International Journal of Molecular Sciences 19, no. 9: 2550. https://doi.org/10.3390/ijms19092550