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Int. J. Mol. Sci. 2018, 19(9), 2549; https://doi.org/10.3390/ijms19092549

Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment

1
Saxon Incubator for Clinical Translation (SIKT), University of Leipzig, 04103 Leipzig, Germany
2
Institute of Veterinary Physiology, University of Leipzig, 04103 Leipzig, Germany
3
Department for Horses, Veterinary Teaching Hospital, University of Leipzig, 04103 Leipzig, Germany
4
Institute of Biotechnology, University of Natural Resources and Life Sciences (BOKU), 1190 Vienna, Austria
*
Author to whom correspondence should be addressed.
Received: 31 July 2018 / Revised: 22 August 2018 / Accepted: 23 August 2018 / Published: 28 August 2018
(This article belongs to the Special Issue Biological Basis of Musculoskeletal Regeneration)
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Abstract

Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represented by supplementation with interleukin-1β and/or tumor necrosis factor-α or by co-culture with allogeneic peripheral blood leukocytes, on ASC functional properties was investigated. High cytokine concentrations increased ASC proliferation and osteogenic differentiation, but decreased chondrogenic differentiation and ASC viability in scaffold culture, as well as tendon scaffold repopulation, and strongly influenced musculoskeletal gene expression. Effects regarding the latter differed between the monolayer and scaffold cultures. Leukocytes rather decreased ASC proliferation, but had similar effects on viability and musculoskeletal gene expression. This included decreased expression of the tenogenic transcription factor scleraxis by an inflammatory environment throughout culture conditions. The data demonstrate that ASC tenogenic properties are compromised in an inflammatory environment, with relevance to their possible mechanisms of action in acute tendon disease. View Full-Text
Keywords: mesenchymal stromal cells (MSC); ASC; tendon; extracellular matrix; bioreactor; inflammation; interleukin-1 (IL-1); tumor necrosis factor-α (TNF-α); leukocytes; co-culture mesenchymal stromal cells (MSC); ASC; tendon; extracellular matrix; bioreactor; inflammation; interleukin-1 (IL-1); tumor necrosis factor-α (TNF-α); leukocytes; co-culture
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Brandt, L.; Schubert, S.; Scheibe, P.; Brehm, W.; Franzen, J.; Gross, C.; Burk, J. Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment. Int. J. Mol. Sci. 2018, 19, 2549.

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