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Article

Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)

1
Botany Department, Faculty of Science, Tanta University, Tanta 31527, Egypt
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UMR CNRS 8256 (B2A), IBPS, Université Paris VI, 75005 Paris, France
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Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
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Timber Trees Research Department, Sabahia Horticulture Research Station, Horticulture Research Institute, Agriculture Research Center, Alexandria 21526, Egypt
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Biological Sciences Department, Faculty of Science, University of Jeddah, Jeddah 21577, Saudi Arabia
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Department of Biology, Xavier University, Cincinnati, OH 45207, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(8), 2433; https://doi.org/10.3390/ijms19082433
Received: 7 June 2018 / Revised: 3 August 2018 / Accepted: 3 August 2018 / Published: 17 August 2018
(This article belongs to the Section Molecular Plant Sciences)
Pisum sativum L. (field pea) is a crop of a high nutritional value and seed oil content. The characterization of pea germplasm is important to improve yield and quality. This study aimed at using fatty acid profiling and amplified fragment length polymorphism (AFLP) markers to evaluate the variation and relationships of 25 accessions of French pea. It also aimed to conduct a marker-trait associations analysis using the crude oil content as the target trait for this analysis, and to investigate whether 5-aminolevulinic acid (ALA) could enhance salt tolerance in the pea germplasm. The percentage of crude oil of the 25 pea genotypes varied from 2.6 to 3.5%, with a mean of 3.04%. Major fatty acids in all of the accessions were linoleic acid. Moreover, the 12 AFLP markers used were polymorphic. The cluster analysis based on fatty acids data or AFLP data divided the 25 pea germplasm into two main clusters. The gene diversity of the AFLP markers varied from 0.21 to 0.58, with a mean of 0.41. Polymorphic information content (PIC) of pea germplasm varied from 0.184 to 0.416 with a mean of 0.321, and their expected heterozygosity (He) varied from 0.212 to 0.477 with a mean of 0.362. The AFLP results revealed that the Nain Ordinaire cultivar has the highest level of genetic variability, whereas Elatius 3 has the lowest level. Three AFLP markers (E-AAC/M-CAA, E-AAC/M-CAC, and E-ACA/M-CAG) were significantly associated with the crude oil content trait. The response of the Nain Ordinaire and Elatius 3 cultivars to high salinity stress was studied. High salinity (150 mM NaCl) slightly reduced the photosynthetic pigments contents in Nain Ordinaire leaves at a non-significant level, however, the pigments contents in the Elatius 3 leaves were significantly reduced by high salinity. Antioxidant enzymes (APX—ascorbate peroxidase; CAT—catalase; and POD—peroxidase) activities were significantly induced in the Nain Ordinaire cultivar, but non-significantly induced in Elatius 3 by high salinity. Priming the salt-stressed Nain Ordinaire and Elatius 3 plants with ALA significantly enhanced the pigments biosynthesis, antioxidant enzymes activities, and stress-related genes expression, as compared to the plants stressed with salt alone. In conclusion, this study is amongst the first investigations that conducted marker-trait associations in pea, and revealed a sort of correlation between the diversity level and salt tolerance. View Full-Text
Keywords: pea; fatty acid; AFLP diversity; marker-trait association; salt tolerance pea; fatty acid; AFLP diversity; marker-trait association; salt tolerance
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MDPI and ACS Style

El-Esawi, M.A.; Al-Ghamdi, A.A.; Ali, H.M.; Alayafi, A.A.; Witczak, J.; Ahmad, M. Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.). Int. J. Mol. Sci. 2018, 19, 2433. https://doi.org/10.3390/ijms19082433

AMA Style

El-Esawi MA, Al-Ghamdi AA, Ali HM, Alayafi AA, Witczak J, Ahmad M. Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.). International Journal of Molecular Sciences. 2018; 19(8):2433. https://doi.org/10.3390/ijms19082433

Chicago/Turabian Style

El-Esawi, Mohamed A., Abdullah A. Al-Ghamdi, Hayssam M. Ali, Aisha A. Alayafi, Jacques Witczak, and Margaret Ahmad. 2018. "Analysis of Genetic Variation and Enhancement of Salt Tolerance in French Pea (Pisum Sativum L.)" International Journal of Molecular Sciences 19, no. 8: 2433. https://doi.org/10.3390/ijms19082433

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