Next Article in Journal
Seed-Specific Gene MOTHER of FT and TFL1 (MFT) Involved in Embryogenesis, Hormones and Stress Responses in Dimocarpus longan Lour.
Next Article in Special Issue
Genome Wide Transcriptome Analysis Reveals Complex Regulatory Mechanisms Underlying Phosphate Homeostasis in Soybean Nodules
Previous Article in Journal
Assessment of Genetic Diversity, Population Structure, and Evolutionary Relationship of Uncharacterized Genes in a Novel Germplasm Collection of Diploid and Allotetraploid Gossypium Accessions Using EST and Genomic SSR Markers
Previous Article in Special Issue
Time-Course of Metabolic and Proteomic Responses to Different Nitrate/Ammonium Availabilities in Roots and Leaves of Maize
Open AccessArticle

Comparative Transcriptome Profiling of Two Tomato Genotypes in Response to Potassium-Deficiency Stress

by Xiaoming Zhao 1,2, Yang Liu 1, Xin Liu 1,* and Jing Jiang 1,*
The Key Laboratory of Protected Horticulture Ministry of Education, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
College of Agriculture, Jilin Agriculture Science and Technology College, Jilin 132101, China
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(8), 2402;
Received: 14 June 2018 / Revised: 9 August 2018 / Accepted: 10 August 2018 / Published: 14 August 2018
Tomato is a crop that requires a sufficient supply of potassium (K) for optimal productivity and quality. K+-deficiency stress decreases tomato yield and quality. To further delve into the mechanism of the response to K+-deficiency and to screen out low-K+ tolerant genes in tomatoes, BGISEQ-500-based RNA sequencing was performed using two tomato genotypes (low-K+ tolerant JZ34 and low-K+ sensitive JZ18). We identified 1936 differentially expressed genes (DEGs) in JZ18 and JZ34 at 12 and 24 h after K+-deficiency treatment. According to the Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses, the DEGs that changed significantly primarily included transcription factors, transporters, kinases, oxidative stress proteins, and hormone signaling-and glycometabolism-related genes. The experimental results confirmed the induced expression of the responsive genes in the low-K+ signaling pathway. The largest group of DEGs comprised up to 110 oxidative stress-related genes. In total, 19 ethylene response factors (ERFs) demonstrated differential expression between JZ18 and JZ34 in response to K+-deficiency. Furthermore, we confirmed 20 DEGs closely related to K+-deficiency stress by quantitative RT-PCR (qRT-PCR), some of which affected the root configuration, these DEGs could be further studied for use as molecular targets to explore novel approaches, and to acquire more effective K acquisition efficiencies for tomatoes. A hypothesis involving possible cross-talk between phytohormone signaling cues and reactive oxygen species (ROS) leading to root growth in JZ34 is proposed. The results provide a comprehensive foundation for the molecular mechanisms involved in the response of tomatoes to low K+ stress. View Full-Text
Keywords: tomato; genotypes; transcriptome; potassium deficiency stress; DEGs; root configuration tomato; genotypes; transcriptome; potassium deficiency stress; DEGs; root configuration
Show Figures

Figure 1

MDPI and ACS Style

Zhao, X.; Liu, Y.; Liu, X.; Jiang, J. Comparative Transcriptome Profiling of Two Tomato Genotypes in Response to Potassium-Deficiency Stress. Int. J. Mol. Sci. 2018, 19, 2402.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop