Genetic and Physio-Biochemical Characterization of a Novel Premature Senescence Leaf Mutant in Rice (Oryza sativa L.)
Abstract
:1. Introduction
2. Results
2.1. Phenotypic Performance of psl85
2.2. Alterations of Chlorophyll Contents, Chloroplast Ultrastructure, and Photosynthetic Parameters
2.3. Impaired Photosynthesis System in psl85 Mutant
2.4. Alterations of Physio-Biochemical Indicators Related to Senescence at Different Growth Stages
2.5. Elevated Expression of Chlorophyll Metabolism and Senescence-Associated Genes by qRT-PCR
2.6. Induction of Synchronous Senescence by Darkness and ABA
2.7. Genetic Analysis and Mapping of PSL85
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Histochemical Analysis
4.3. Chlorophyll Content and Membrane Ion Leakage Determination
4.4. Senescence-Related and Photosynthesis Parameter Measurement
4.5. TUNEL Assay
4.6. Transmission Electron Microscopy and Paraffin Section
4.7. Darkness- and ABA-Induced Leaf Senescence
4.8. ABA Level Analysis
4.9. Gene Expression Analysis
4.10. SDS-PAGE and Immunoblot Analysis
4.11. Genetic Analysis and Gene Mapping
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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ORF | Gene ID | Annotation |
---|---|---|
ORF1 | LOC_Os07g10610 | Expressed protein |
ORF2 | LOC_Os07g10620 | Expressed protein |
ORF3 | LOC_Os07g10630 | Expressed protein |
ORF4 | LOC_Os07g10640 | Hypothetical protein |
ORF5 | LOC_Os07g10650 | Hypothetical protein |
ORF6 | LOC_Os07g10660 | Ribosomal protein, putative, expressed |
ORF7 | LOC_Os07g10670 | Expressed protein |
ORF8 | LOC_Os07g10680 | Polygalacturonase, putative, expressed |
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He, Y.; Zhang, Z.; Li, L.; Tang, S.; Wu, J.-L. Genetic and Physio-Biochemical Characterization of a Novel Premature Senescence Leaf Mutant in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2018, 19, 2339. https://doi.org/10.3390/ijms19082339
He Y, Zhang Z, Li L, Tang S, Wu J-L. Genetic and Physio-Biochemical Characterization of a Novel Premature Senescence Leaf Mutant in Rice (Oryza sativa L.). International Journal of Molecular Sciences. 2018; 19(8):2339. https://doi.org/10.3390/ijms19082339
Chicago/Turabian StyleHe, Yan, Zhihong Zhang, Liangjian Li, Shaoqing Tang, and Jian-Li Wu. 2018. "Genetic and Physio-Biochemical Characterization of a Novel Premature Senescence Leaf Mutant in Rice (Oryza sativa L.)" International Journal of Molecular Sciences 19, no. 8: 2339. https://doi.org/10.3390/ijms19082339