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Int. J. Mol. Sci. 2018, 19(7), 1991; https://doi.org/10.3390/ijms19071991

Gold Nanoparticles: A Powerful Tool to Visualize Proteins on Ordered Mesoporous Silica and for the Realization of Theranostic Nanobioconjugates

1
Department of Biomedical Science, University of Cagliari, Monserrato, CA 09042, Italy
2
Department of Chemistry, CSGI, University of Florence, Sesto Fiorentino, FI 50019, Italy
3
Department of Chemical and Geological Sciences, CSGI, University of Cagliari, Monserrato, CA 90042, Italy
*
Authors to whom correspondence should be addressed.
Received: 21 June 2018 / Revised: 2 July 2018 / Accepted: 5 July 2018 / Published: 8 July 2018
(This article belongs to the Special Issue Translating Gold Nanoparticles to Diagnostics and Therapeutics)
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Abstract

Ordered mesoporous silica (OMS) is a very interesting nanostructured material for the design and engineering of new target and controlled drug-delivery systems. Particularly relevant is the interaction between OMS and proteins. Large pores (6–9 nm) micrometric particles can be used for the realization of a drug depot system where therapeutic proteins are adsorbed either inside the mesopores or on the external surface. Small pores (1–2 nm) mesoporous silica nanoparticles (MSNs), can be injected in the blood stream. In the latter case, therapeutic proteins are mainly adsorbed on the MSNs’ external surface. Whenever a protein-OMS conjugate is prepared, a diagnostic method to locate the protein either on the internal or the external silica surface is of utmost importance. To visualize the fine localization of proteins adsorbed in mesoporous silica micro- and nanoparticles, we have employed specific transmission electron microscopy (TEM) analytical strategies based on the use of gold nanoparticles (GNPs) conjugates. GNPs are gaining in popularity, representing a fundamental tool to design future applications of MSNs in nanomedicine by realizing theranostic nanobioconjugates. It may be pointed out that we are at the very beginning of a new age of the nanomaterial science: the “mesoporous golden age”. View Full-Text
Keywords: ordered mesoporous materials; immunogold staining; gold nanoparticles; protein localization; nanomedicine; theranostics; nanobioconjugate ordered mesoporous materials; immunogold staining; gold nanoparticles; protein localization; nanomedicine; theranostics; nanobioconjugate
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Piludu, M.; Medda, L.; Monduzzi, M.; Salis, A. Gold Nanoparticles: A Powerful Tool to Visualize Proteins on Ordered Mesoporous Silica and for the Realization of Theranostic Nanobioconjugates. Int. J. Mol. Sci. 2018, 19, 1991.

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