Radiation Sensitivity of Adipose-Derived Stem Cells Isolated from Breast Tissue
AbstractWithin their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology. View Full-Text
Share & Cite This Article
Baaße, A.; Machoy, F.; Juerß, D.; Baake, J.; Stang, F.; Reimer, T.; Krapohl, B.D.; Hildebrandt, G. Radiation Sensitivity of Adipose-Derived Stem Cells Isolated from Breast Tissue. Int. J. Mol. Sci. 2018, 19, 1988.
Baaße A, Machoy F, Juerß D, Baake J, Stang F, Reimer T, Krapohl BD, Hildebrandt G. Radiation Sensitivity of Adipose-Derived Stem Cells Isolated from Breast Tissue. International Journal of Molecular Sciences. 2018; 19(7):1988.Chicago/Turabian Style
Baaße, Annemarie; Machoy, Friederike; Juerß, Dajana; Baake, Jana; Stang, Felix; Reimer, Toralf; Krapohl, Björn D.; Hildebrandt, Guido. 2018. "Radiation Sensitivity of Adipose-Derived Stem Cells Isolated from Breast Tissue." Int. J. Mol. Sci. 19, no. 7: 1988.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.