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Int. J. Mol. Sci. 2018, 19(7), 1988; https://doi.org/10.3390/ijms19071988

Radiation Sensitivity of Adipose-Derived Stem Cells Isolated from Breast Tissue

1
Department of Radiotherapy and Radiation Oncology, University Medical Centre Rostock, Suedring 75, 18059 Rostock, Germany
2
Clinic for Plastic, Hand and Reconstructive Surgery, University Hospital Schleswig-Holstein, Campus Luebeck. Ratzeburger Allee 160, 23538 Luebeck, Germany
3
Department of Obstetrics and Gynecology, University of Rostock, Women’s Hospital, Suedring 81, 18059 Rostock, Germany
4
Berliner Centrum für Musikermedizin, Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany
*
Author to whom correspondence should be addressed.
Received: 28 May 2018 / Revised: 26 June 2018 / Accepted: 5 July 2018 / Published: 7 July 2018
(This article belongs to the Special Issue Adipose Stem Cells)
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Abstract

Within their niche, adipose-derived stem cells (ADSCs) are essential for homeostasis as well as for regeneration. Therefore, the interest of physicians is to use ADSCs as a tool for radiation oncology and regenerative medicine. To investigate related risks, this study analyses the radiation response of adult stem cells isolated from the adipose tissue of the female breast. To avoid donor-specific effects, ADSCs isolated from breast reduction mammoplasties of 10 donors were pooled and used for the radiobiological analysis. The clonogenic survival fraction assay was used to classify the radiation sensitivity in comparison to a more radiation-sensitive (ZR-75-1), moderately sensitive (MCF-7), and resistant (MCF10A) cell lines. Afterwards, cytotoxicity and genotoxicity of irradiation on ADSCs were investigated. On the basis of clonogenic cell survival rates of ADSCs after irradiation, we assign ADSCs an intermediate radiation sensitivity. Furthermore, a high repair capacity of double-strand breaks is related to an altered cell cycle arrest and increased expression of cyclin-dependent kinase (CDK) inhibitor p21. ADSCs isolated from breast tissue exhibit intermediate radiation sensitivity, caused by functional repair mechanisms. Therefore, we propose ADSCs to be a promising tool in radiation oncology. View Full-Text
Keywords: adipose-derived stem cells; radiation therapy; ionizing radiation; regenerative medicine adipose-derived stem cells; radiation therapy; ionizing radiation; regenerative medicine
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Baaße, A.; Machoy, F.; Juerß, D.; Baake, J.; Stang, F.; Reimer, T.; Krapohl, B.D.; Hildebrandt, G. Radiation Sensitivity of Adipose-Derived Stem Cells Isolated from Breast Tissue. Int. J. Mol. Sci. 2018, 19, 1988.

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