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Int. J. Mol. Sci. 2018, 19(6), 1558; https://doi.org/10.3390/ijms19061558

Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis

1
CNRS ERL 7003, Laboratoire “Signalisation & Transports Ioniques Membranaires”, University of Poitiers, 86073 Poitiers, France
2
CNRS UMR 7285, Institut de Chimie des Milieux et des Matériaux de Poitiers (IC2MP), University of Poitiers, 86073 Poitiers, France
3
CHU de Poitiers, 86021 Poitiers, France
*
Author to whom correspondence should be addressed.
Received: 27 April 2018 / Revised: 17 May 2018 / Accepted: 22 May 2018 / Published: 24 May 2018
(This article belongs to the Special Issue Interplay of Connexins and Pannexins in Tissue Function and Disease)
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Abstract

The molecular mechanisms governing the formation of lymphatic vasculature are not yet well understood. Pannexins are transmembrane proteins that form channels which allow for diffusion of ions and small molecules (<1 kDa) between the extracellular space and the cytosol. The expression and function of pannexins in blood vessels have been studied in the last few decades. Meanwhile, no studies have been conducted to evaluate the role of pannexins during human lymphatic vessel formation. Here we show, using primary human dermal lymphatic endothelial cells (HDLECs), pharmacological tools (probenecid, Brilliant Blue FCF, mimetic peptides [10Panx]) and siRNA-mediated knockdown that Pannexin-1 is necessary for capillary tube formation on Matrigel and for VEGF-C-induced invasion. These results newly identify Pannexin-1 as a protein highly expressed in HDLECs and its requirement during in vitro lymphangiogenesis. View Full-Text
Keywords: lymphatic endothelial cells; pannexins; Panx1; lymphangiogenesis; cell invasion; Vascular Endothelial Growth Factor-C (VEGF-C) lymphatic endothelial cells; pannexins; Panx1; lymphangiogenesis; cell invasion; Vascular Endothelial Growth Factor-C (VEGF-C)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Boucher, J.; Simonneau, C.; Denet, G.; Clarhaut, J.; Balandre, A.-C.; Mesnil, M.; Cronier, L.; Monvoisin, A. Pannexin-1 in Human Lymphatic Endothelial Cells Regulates Lymphangiogenesis. Int. J. Mol. Sci. 2018, 19, 1558.

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