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Int. J. Mol. Sci. 2018, 19(4), 1162; https://doi.org/10.3390/ijms19041162

CA 19-9 Pancreatic Tumor Marker Fluorescence Immunosensing Detection via Immobilized Carbon Quantum Dots Conjugated Gold Nanocomposite

1
Department of Chemistry, College of Science, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia
2
General Administration and Medical Affairs, Zagazig University, Zagazig 44511, Egypt
3
Department of Agronomy, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt
*
Author to whom correspondence should be addressed.
Received: 13 March 2018 / Revised: 8 April 2018 / Accepted: 10 April 2018 / Published: 11 April 2018
(This article belongs to the Special Issue Translating Gold Nanoparticles to Diagnostics and Therapeutics)
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Abstract

The clinical detection of carbohydrate antigen 19-9 (CA 19-9), a tumor marker in biological samples, improves and facilitates the rapid screening and diagnosis of pancreatic cancer. A simple, low cost, fast, and green synthesis method to prepare a viable carbon quantum dots/gold (CQDs/Au) nanocomposite fluorescence immunosensing solution for the detection of CA 19-9 was reported. The present method is conducted by preparing glucose-derived CQDs using a microwave-assisted method. CQDs were employed as reducing and stabilizing agents for the preparation of a CQDs/Au nanocomposite. The immobilized anti-CA 19-9-labeled horseradish peroxidase enzyme (Ab–HRP) was anchored to the surface of a CQDs/Au nanocomposite by a peptide interaction between the carboxylic and amine active groups. The CA 19-9 antigen was trapped by another monoclonal antibody that was coated on the surface of microtiter wells. The formed sandwich capping antibody–antigen–antibody enzyme complex had tunable fluorescence properties that were detected under excitation and emission wavelengths of 420 and 530 nm. The increase in fluorescence intensities of the immunoassay sensing solution was proportional to the CA 19-9 antigen concentration in the linear range of 0.01–350 U mL−1 and had a lower detection limit of 0.007 U mL−1. The proposed CQDs/Au nanocomposite immunoassay method provides a promising tool for detecting CA 19-9 in human serum. View Full-Text
Keywords: carbon quantum dots; tumor markers; gold nanocomposite; CA 19-9 antigen; immunoassay technique carbon quantum dots; tumor markers; gold nanocomposite; CA 19-9 antigen; immunoassay technique
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Alarfaj, N.A.; El-Tohamy, M.F.; Oraby, H.F. CA 19-9 Pancreatic Tumor Marker Fluorescence Immunosensing Detection via Immobilized Carbon Quantum Dots Conjugated Gold Nanocomposite. Int. J. Mol. Sci. 2018, 19, 1162.

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