Next Article in Journal
The Role of Macrophages in Neuroinflammatory and Neurodegenerative Pathways of Alzheimer’s Disease, Amyotrophic Lateral Sclerosis, and Multiple Sclerosis: Pathogenetic Cellular Effectors and Potential Therapeutic Targets
Next Article in Special Issue
DNA Damage, Mutagenesis and Cancer
Previous Article in Journal
Fraxinellone Attenuates Rheumatoid Inflammation in Mice
Previous Article in Special Issue
A Case-Control Study of the Genetic Variability in Reactive Oxygen Species—Metabolizing Enzymes in Melanoma Risk
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(3), 830; https://doi.org/10.3390/ijms19030830

High NOTCH1 mRNA Expression Is Associated with Better Survival in HNSCC

1
Department of Otolaryngology-Head and Neck Surgery, Technical University of Munich, Ismaninger Straße 22, 81675 Muenchen, Germany
2
Institute of Medical Informatics Statistics and Epidemiology, Technical University of Munich, Ismaninger Straße 22, 81675 Muenchen, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 25 February 2018 / Revised: 4 March 2018 / Accepted: 6 March 2018 / Published: 13 March 2018
(This article belongs to the Special Issue Chemically-Induced DNA Damage, Mutagenesis, and Cancer)
Full-Text   |   PDF [1520 KB, uploaded 13 March 2018]   |  

Abstract

The clinical impact of the expression of NOTCH1 signaling components in squamous cell carcinoma of the pharynx and larynx has only been evaluated in subgroups. The aim of this study was therefore to evaluate NOTCH1 expression in head and neck squamous cell cancer (HNSCC) patient tissue and cell lines. We analyzed tissue from 195 HNSCCs and tissue from 30 normal patients for mRNA expression of NOTCH1, NOTCH3, HES1, HEY1, and JAG1 using quantitative real-time PCR. Association of expression results and clinical orpathological factors was examined with multivariate Cox regression. NOTCH1 expression was determined in three Human Papilloma Virus (HPV)-positive and nine HPV-negative HNSCC cell lines. High expression of NOTCH1 was associated with better overall survival (p = 0.013) and disease-free survival (p = 0.040). Multivariate Cox regression confirmed the significant influence of NOTCH1 expression on overall survival (p = 0.033) and disease-free survival (p = 0.029). A significant correlation was found between p16 staining and NOTCH1 mRNA expression (correlation coefficient 0.28; p = 0.01). NOTCH1 was expressed at higher levels in HPV-positive HNSCC cell lines compared with HPV-negative cell lines, which was not statistically significant (p = 0.068). We conclude that NOTCH1 expression is associated with overall survival, and that inhibition of NOTCH1 therefore seems less promising. View Full-Text
Keywords: NOTCH signaling; HNSCC; overall survival NOTCH signaling; HNSCC; overall survival
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Wirth, M.; Jira, D.; Ott, A.; Piontek, G.; Pickhard, A. High NOTCH1 mRNA Expression Is Associated with Better Survival in HNSCC. Int. J. Mol. Sci. 2018, 19, 830.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top