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Int. J. Mol. Sci. 2018, 19(3), 793;

Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study

School of Energy and Power Engineering, Wuhan University of Technology, Wuhan 430063, China
Institute for Science and Technology in Medicine, Keele University, Stoke-on-Trent ST4 7QB, UK
Authors to whom correspondence should be addressed.
Received: 24 February 2018 / Revised: 7 March 2018 / Accepted: 7 March 2018 / Published: 9 March 2018
(This article belongs to the Section Materials Science)
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Modification of metal surfaces with antimicrobial peptides is a promising approach to reduce bacterial adhesion. Here, cyclic peptides or cycloids, possessing remarkable stability and antimicrobial activities, were extracted and purified from Viola philippica Cav., and identified using mass spectrometry. Cyclotides were subsequently utilized to modify stainless steel surfaces via polydopamine-mediated coupling. The resulting cyclotide-modified surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy and contact angle analysis. The antibacterial capacity of these cyclotides against Staphylococcus aureus was assessed by Alamar blue assay. The antibiofilm capacity of the modified surfaces was assessed by crystal violet assay, and scanning electron microscopy (SEM). A composite of Kalata b1, Varv A, Viba 15 and Viba 17 (P1); Varv E (P2); and Viphi G (P3) were isolated and identified. FTIR analysis of the modified surfaces demonstrated that cyclotides bound to the surfaces and induced reduction of contact angles. Antimicrobial effects showed an order P3 > P1 and P2, with P3-treated surfaces demonstrating the strongest antibiofilm capacity. SEM confirmed reduced biofilm formation for P3-treated surfaces. This study provides novel evidence for cyclotides as a new class for development of antibacterial and antibiofilm agents. View Full-Text
Keywords: cyclotides; surface modification; antibiofilm; antibacterial; polydopamine cyclotides; surface modification; antibiofilm; antibacterial; polydopamine

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Cao, P.; Yang, Y.; Uche, F.I.; Hart, S.R.; Li, W.-W.; Yuan, C. Coupling Plant-Derived Cyclotides to Metal Surfaces: An Antibacterial and Antibiofilm Study. Int. J. Mol. Sci. 2018, 19, 793.

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