Next Article in Journal
Functional Association between Regulatory RNAs and the Annexins
Next Article in Special Issue
Immunomodulatory Function of Myeloid-Derived Suppressor Cells during B Cell-Mediated Immune Responses
Previous Article in Journal
New Insights into the Immune Molecular Regulation of the Pathogenesis of Acute Respiratory Distress Syndrome
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(2), 589; https://doi.org/10.3390/ijms19020589

Peripheral B-Cell Subset Distribution in Primary Antiphospholipid Syndrome

1
Transplantation and Autoimmunity Laboratory, Rheumatology Department, University Hospital Marqués de Valdecilla-IDIVAL, 39008 Santander, Spain
2
Rheumatology Department, Hospital Sierrallana, 39300 Torrelavega, Spain
3
Rheumatology Department, University Hospital Araba, 01004 Vitoria, Spain
4
Immunology Department, University Hospital Marqués de Valdecilla-IDIVAL, Faculty of Medicine, Cantabria University, 39008 Santander, Spain
5
Rheumatology Department, University Hospital Marqués de Valdecilla-IDIVAL, Faculty of Medicine, Cantabria University, 39008 Santander, Spain
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 January 2018 / Revised: 9 February 2018 / Accepted: 14 February 2018 / Published: 16 February 2018
(This article belongs to the Special Issue B Cells and Immunological Tolerance)
Full-Text   |   PDF [1694 KB, uploaded 23 February 2018]   |  

Abstract

Background: B-cell differentiation and B-cell tolerance checkpoints may be different in antiphospholipid syndrome (APS) from systemic lupus erythematosus (SLE) and can help to understand differences between them. Our aim was to define alterations of B-cell subsets in patients with primary APS (pAPS) and to compare them with SLE patients and healthy controls (HC). Methods: Cross-sectional study including three study groups: 37 patients with pAPS, 11 SLE patients, and 21 age- and gender-matched HC. We determined the frequencies of different B-cell subsets in peripheral blood naïve and memory compartments. In addition, we measured serum B cell-activating factor (BAFF) levels and circulating pro-inflammatory cytokines, such as IL-6, by commercial ELISA and CBA, respectively. Results: Patients with pAPS showed a lower percentage of immature and naïve B cells than patients with SLE (p = 0.013 and p = 0.010, respectively) and a higher percentage of non-switched memory B cells than patients with SLE (p = 0.001). No differences either in the percentage of switched memory cells or plasma cells were found among the different groups. Serum BAFF levels were higher in SLE patients than in healthy controls and pAPS patients (p = 0.001 and p = 0.017, respectively). A significant increase in the serum BAFF levels was also observed in pAPS patients compared to HC (p = 0.047). Circulating IL-6 levels were higher in SLE and pAPS patients than HC (p = 0.036 and p = 0.048, respectively). A positive correlation was found between serum BAFF and IL-6 levels in patients with SLE but not in pAPS (p = 0.011). Conclusions: Our characterization of peripheral blood B-cell phenotypes in pAPS demonstrates different frequencies of circulating B cells at different stages of differentiation. These differences in the naïve B-cell repertoire could explain the higher number and variety of autoantibodies in SLE patients in comparison to pAPS patients, especially in those with obstetric complications. View Full-Text
Keywords: APS; SLE; B cell; BAFF; autoimmunity; IL-6; inflammation APS; SLE; B cell; BAFF; autoimmunity; IL-6; inflammation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Álvarez-Rodríguez, L.; Riancho-Zarrabeitia, L.; Calvo-Alén, J.; López-Hoyos, M.; Martínez-Taboada, V. Peripheral B-Cell Subset Distribution in Primary Antiphospholipid Syndrome. Int. J. Mol. Sci. 2018, 19, 589.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top