Acidic Chitinase-Chitin Complex Is Dissociated in a Competitive Manner by Acetic Acid: Purification of Natural Enzyme for Supplementation Purposes
Abstract
:1. Introduction
2. Results
2.1. Dissociation of Chicken Chia–Chitin Complex by 0.1 M Acetic Acid
2.2. Acetic Acid Is Suitable for Chicken Chia Elution from Chitin Column without Acid Denaturation
2.3. Effect of Acetic Acid on Chitin Degradation by Chicken Chia Enzyme
2.4. CatD and CBD of Chicken Chia Bind with Chitin Column, Which Were Eluted by Acetic Acid
2.5. Pig Chia Purification from Stomach Tissues Using a Chitin Column and Acetic Acid
3. Discussion
4. Materials and Methods
4.1. Chicken Glandular Stomach and Pig Stomach Tissues
4.2. Preparation of Soluble Proteins from Chicken Glandular Stomach and Pig Stomach
4.3. Elution of Chia Proteins from Chitin Column
4.4. Purification of Chicken and Pig Chia Using 8 M Urea or 0.1 M Acetic Acid
4.5. SDS-Polyacrylamide Gel Electrophoresis and SYPRO Ruby Staining
4.6. Differential Scanning Fluorimetry
4.7. Fourier Transform Infrared Spectroscopy
4.8. Chitinase Enzymatic Assays
4.9. Degradation of Chitin Beads, Colloidal and Crystalline Chitin Substrates by Chia
4.10. E. coli Expression Vectors and Preparation of Recombinant Fusion Proteins
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Tabata, E.; Kashimura, A.; Wakita, S.; Sakaguchi, M.; Sugahara, Y.; Imamura, Y.; Shimizu, H.; Matoska, V.; Bauer, P.O.; Oyama, F. Acidic Chitinase-Chitin Complex Is Dissociated in a Competitive Manner by Acetic Acid: Purification of Natural Enzyme for Supplementation Purposes. Int. J. Mol. Sci. 2018, 19, 362. https://doi.org/10.3390/ijms19020362
Tabata E, Kashimura A, Wakita S, Sakaguchi M, Sugahara Y, Imamura Y, Shimizu H, Matoska V, Bauer PO, Oyama F. Acidic Chitinase-Chitin Complex Is Dissociated in a Competitive Manner by Acetic Acid: Purification of Natural Enzyme for Supplementation Purposes. International Journal of Molecular Sciences. 2018; 19(2):362. https://doi.org/10.3390/ijms19020362
Chicago/Turabian StyleTabata, Eri, Akinori Kashimura, Satoshi Wakita, Masayoshi Sakaguchi, Yasusato Sugahara, Yasutada Imamura, Hideaki Shimizu, Vaclav Matoska, Peter O. Bauer, and Fumitaka Oyama. 2018. "Acidic Chitinase-Chitin Complex Is Dissociated in a Competitive Manner by Acetic Acid: Purification of Natural Enzyme for Supplementation Purposes" International Journal of Molecular Sciences 19, no. 2: 362. https://doi.org/10.3390/ijms19020362