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Open AccessArticle

Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons

1
U1192-Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), Univ. Lille, INSERM, F-59000 Lille, France
2
DARIS Centre for Scientific Research and Technology Development, University of Nizwa, P.O. Box 33, Birkat Al-Mouz, PC 616 Nizwa, Oman
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2018, 19(12), 4124; https://doi.org/10.3390/ijms19124124
Received: 27 October 2018 / Revised: 13 December 2018 / Accepted: 17 December 2018 / Published: 19 December 2018
(This article belongs to the Special Issue Neuroimmunology)
In healthy or pathological brains, the neuroinflammatory state is supported by a strong communication involving microglia and neurons. Recent studies indicate that extracellular vesicles (EVs), including exosomes and microvesicles, play a key role in the physiological interactions between cells allowing central nervous system (CNS) development and/or integrity. The present report used medicinal leech CNS to investigate microglia/neuron crosstalk from ex vivo approaches as well as primary cultures. The results demonstrated a large production of exosomes from microglia. Their incubation to primary neuronal cultures showed a strong interaction with neurites. In addition, neurite outgrowth assays demonstrated microglia exosomes to exhibit significant neurotrophic activities using at least a Transforming Growth Factor beta (TGF-β) family member, called nGDF (nervous Growth/Differentiation Factor). Of interest, the results also showed an EV-mediated dialog between leech microglia and rat cells highlighting this communication to be more a matter of molecules than of species. Taken together, the present report brings a new insight into the microglia/neuron crosstalk in CNS and would help deciphering the molecular evolution of such a cell communication in brain. View Full-Text
Keywords: microglia; exosomes; neurite outgrowth; leech microglia; exosomes; neurite outgrowth; leech
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MDPI and ACS Style

Raffo-Romero, A.; Arab, T.; Al-Amri, I.S.; Le Marrec-Croq, F.; Van Camp, C.; Lemaire, Q.; Salzet, M.; Vizioli, J.; Sautiere, P.-E.; Lefebvre, C. Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons. Int. J. Mol. Sci. 2018, 19, 4124. https://doi.org/10.3390/ijms19124124

AMA Style

Raffo-Romero A, Arab T, Al-Amri IS, Le Marrec-Croq F, Van Camp C, Lemaire Q, Salzet M, Vizioli J, Sautiere P-E, Lefebvre C. Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons. International Journal of Molecular Sciences. 2018; 19(12):4124. https://doi.org/10.3390/ijms19124124

Chicago/Turabian Style

Raffo-Romero, Antonella; Arab, Tanina; Al-Amri, Issa S.; Le Marrec-Croq, Francoise; Van Camp, Christelle; Lemaire, Quentin; Salzet, Michel; Vizioli, Jacopo; Sautiere, Pierre-Eric; Lefebvre, Christophe. 2018. "Medicinal Leech CNS as a Model for Exosome Studies in the Crosstalk between Microglia and Neurons" Int. J. Mol. Sci. 19, no. 12: 4124. https://doi.org/10.3390/ijms19124124

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