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Open AccessArticle

Dental Pulp Stem Cell-Derived Factors Alleviate Subarachnoid Hemorrhage-Induced Neuroinflammation and Ischemic Neurological Deficits

Department of surgery, Division of Neurosurgery, National Taiwan University Hospital, Taipei 100, Taiwan
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan
Department of Neurosurgery, Tri-Service General Hospital, Taipei 115, Taiwan
Non-invasive Cancer Therapy Research Institute - Taiwan, Taipei 104, Taiwan
Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan
Department of Medical Research, Taipei Veterans General Hospital, Taipei 112, Taiwan
Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei 102, Taiwan
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(15), 3747;
Received: 11 June 2019 / Revised: 25 July 2019 / Accepted: 30 July 2019 / Published: 31 July 2019
(This article belongs to the Special Issue Neuroimmunology)
PDF [3173 KB, uploaded 31 July 2019]
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Aneurysmal subarachnoid hemorrhage (aSAH), characterized by the extravasation of blood into the subarachnoid space caused by an intracranial aneurysm rupture, may lead to neurocognitive impairments and permanent disability and usually carries poor outcome. Dental or gingiva-derived stem cells have been shown to contribute to immune modulation and neuroregeneration, but the underlying mechanisms are unclear. In the present study, we sought to investigate whether dental pulp stem cells (DPSCs) secrete certain factor(s) that can ameliorate the neural damage and other manifestations in a rat aSAH model. Twenty-four hours after the induction of aSAH, microthrombosis, cortical vasoconstriction, and the decrease in microcirculation and tissue oxygen pressure were detected. Intrathecal administration of DPSC-derived conditioned media (DPSC-CM) ameliorated aSAH-induced vasoconstriction, neuroinflammation, and improved the oxygenation in the injured brain. Rotarod test revealed that the aSAH-induced cognitive and motor impairments were significantly improved by this DPSC-CM administration. Cytokine array indicated the major constituent of DPSC-CM was predominantly insulin growth factor-1 (IGF-1). Immunohistochemistry staining of injured brain tissue revealed the robust increase in Iba1-positive cells that were also ameliorated by DPSC-CM administration. Antibody-mediated neutralization of IGF-1 moderately deteriorated the rescuing effect of DPSC-CM on microcirculation, Iba1-positive cells in the injured brain area, and the cognitive/motor impairments. Taken together, the DPSC-derived secretory factors showed prominent therapeutic potential for aSAH. This therapeutic efficacy may include improvement of microcirculation, alleviation of neuroinflammation, and microglial activation; partially through IGF-1-dependent mechanisms. View Full-Text
Keywords: aneurysmal subarachnoid hemorrhage; stem cell; neuroinflammation; conditioned medium aneurysmal subarachnoid hemorrhage; stem cell; neuroinflammation; conditioned medium

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Chen, T.-F.; Chen, K.-W.; Chien, Y.; Lai, Y.-H.; Hsieh, S.-T.; Ma, H.-Y.; Wang, K.-C.; Shiau, C.-Y. Dental Pulp Stem Cell-Derived Factors Alleviate Subarachnoid Hemorrhage-Induced Neuroinflammation and Ischemic Neurological Deficits. Int. J. Mol. Sci. 2019, 20, 3747.

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