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Int. J. Mol. Sci. 2018, 19(12), 3971; https://doi.org/10.3390/ijms19123971

Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells

1
Department of Oral Physiology, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50610, Korea
2
Department of Dental Pharmacology, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan 50610, Korea
*
Author to whom correspondence should be addressed.
Received: 2 October 2018 / Revised: 23 November 2018 / Accepted: 6 December 2018 / Published: 10 December 2018
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Abstract

Gastrin-releasing peptide (GRP), a member of bombesin-like peptides, and its receptor (GRP-R) play an important role in various physiological and pathological conditions. In this work, we investigated the role of GRP-R on adipogenesis in 3T3-L1 adipocytes. The expression of GRP-R was significantly increased during the adipocyte differentiation of 3T3-L1 cells. The inhibition of GRP-R by the antagonist RC-3095 affected adipogenesis in 3T3-L1 cells, which reduced lipid accumulation and regulated the expression of adipogenic genes. Moreover, cyclic AMP response element-binding protein (CREB) directly bound to the GRP-R promoter upon exposure to adipogenic stimuli. The down-regulation of GRP-R by the knockdown of CREB inhibited adipocyte differentiation of 3T3-L1 cells. Together these results suggest that the regulation of GRP-R activity or expression has an influence on adipogenesis through regulating adipogenic related genes. View Full-Text
Keywords: gastrin-releasing peptide; gastrin-releasing peptide receptor; adipocyte differentiation gastrin-releasing peptide; gastrin-releasing peptide receptor; adipocyte differentiation
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Kim, M.-K.; Park, H.-J.; Kim, Y.; Bae, S.-K.; Kim, H.J.; Bae, M.-K. Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells. Int. J. Mol. Sci. 2018, 19, 3971.

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