Open Access This article is
- freely available
Int. J. Mol. Sci. 2018, 19(11), 3402; https://doi.org/10.3390/ijms19113402
Targeting Cytokines as Evolving Treatment Strategies in Chronic Inflammatory Airway Diseases
Division of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, The University of Alabama at Birmingham, Birmingham, AL 35294, USA
Author to whom correspondence should be addressed.
Received: 28 September 2018 / Accepted: 27 October 2018 / Published: 30 October 2018
Cytokines are key players in the initiation and propagation of inflammation in chronic inflammatory airway diseases such as chronic obstructive pulmonary disease (COPD), bronchiectasis and allergic asthma. This makes them attractive targets for specific novel anti-inflammatory treatment strategies. Recently, both interleukin-1 (IL-1) and IL-6 have been associated with negative health outcomes, mortality and a pro-inflammatory phenotype in COPD. IL-6 in COPD was shown to correlate negatively with lung function, and IL-1beta was induced by cigarette smoke in the bronchial epithelium, causing airway inflammation. Furthermore, IL-8 has been shown to be a pro-inflammatory marker in bronchiectasis, COPD and allergic asthma. Clinical trials using specific cytokine blockade therapies are currently emerging and have contributed to reduce exacerbations and steroid use in COPD. Here, we present a review of the current understanding of the roles of cytokines in the pathophysiology of chronic inflammatory airway diseases. Furthermore, outcomes of clinical trials in cytokine blockade as novel treatment strategies for selected patient populations with those diseases will be discussed.
Keywords:COPD; allergic asthma; bronchiectasis; cytokines; IL-1β; IL-6; IL-8
Chronic respiratory diseases account for 4.7% of global disability-adjusted life years with asthma and chronic obstructive pulmonary disease (COPD) being the most frequent, and chronic airway inflammation being one of the key processes involved in their pathogenesis [1,2].
Since the lung is constantly exposed to the environment and harmful pathogens, inflammation, as an immediate defense mechanism, is required to eliminate these noxious stimuli as early as possible. For a long time, inflammatory cells were thought to be the key orchestrators of the immune homeostasis in the lung, with dendritic cells as antigen-presenting cells and macrophages as the first line of defense. Macrophages are located in airways, alveoli and the lung interstitium, and have the ability to migrate to the lung microvasculature, thereby modulating acute and chronic inflammatory responses . They are also a main source of cytokines and inflammatory mediators, in addition to phagocytosing bacteria and apoptotic cells, and promote neutrophil accumulation .
Neutrophils have been seen as the second line of defense. They migrate out of the pulmonary capillaries and into the air spaces and kill ingested opportunistic microbes (fungi, protozoa, bacteria, viruses) .
Lymphocytes are found in both airways and lung parenchyma and are divided into two major populations: thymus-derived T cells and bone marrow-derived B cells. T cells have two major subsets—cluster of differentiation (CD)4+ and CD8+—with further division into Th1 (cellular immunity) and Th2 (humoral immunity). Th2 cytokines include IL-4, IL-5, IL-9, and IL-13, and they promote secretion of immunoglobulin (Ig)E and eosinophilic responses. Therefore, a dysregulated Th1/Th2 response has been linked to a diversity of chronic inflammatory conditions, such as asthma and chronic bronchitis . Th17 is a third subset of Th cells, which are characterized by their production of IL-17. They also play an important role in host defense and several inflammatory disorders .
Although inflammatory cells are essential in modulating acute and chronic airway inflammation, epithelial, endothelial and mesenchymal cells also participate in the inflammatory process [8,9]. Recent genetic, structural and functional studies have identified the respiratory epithelium as a main contributor in the development of inflammatory airway disorders . The airway epithelium functions by responding to environmental stimuli, not only via leukocyte recruitment, but also through secretion of chemokines, cytokines, and antimicrobial peptides [11,12,13,14]. The bronchial mucosa consists of a muco-ciliated, pseudostratified epithelium with predominantly ciliated cells, as well as mucus-secreting goblet cells (5–15%) in the large airways and basal cells/club cells in the smaller airways [15,16,17]. It represents a physical and chemical barrier by: (1) apical junctions lining the upper and lower airways, (2) mucociliary clearance, and (3) secretion of mediators regulating inflammation, chemotaxis, and antimicrobial defense as mentioned above [18,19,20,21,22]. In addition, airway epithelial cells also initiate and regulate the innate and adaptive immunity of the lung via pattern-recognition receptors and trans-epithelial immunoglobulin transport . In chronic inflammatory airway diseases, epithelial dysfunction is one of the key features leading to altered epithelial integrity and disrupted physical, chemical, and immune barrier functions .
2. Mediators of Chronic Inflammatory Airway Diseases
Cytokines are polypeptides and secreted by various cell types, including inflammatory cells and the airway epithelium . They have autocrine, paracrine, or endocrine functions to regulate immunity and inflammation. Chemokines are cytokines that serve as chemotactic agents or chemoattractants for other cells. Furthermore, there is redundancy in cytokine-induced effects with different cytokines acting either synergistically or antagonistically. Interleukins are a subgroup of cytokines and can be classified as pro- or anti-inflammatory. Key pro-inflammatory interleukins are IL-1β, IL-6, and IL-8; they can activate the immune system and contribute to the acute inflammatory response by promoting antigen presentation, inflammatory cell activation, and expression of enzymes involved in matrix degradation . Important anti-inflammatory interleukins include IL-1ra and IL-10, which can be secreted by alveolar macrophages and attenuate lung inflammation [26,27]. IL-10 can also inhibit the production of pro-inflammatory cytokines by activation of T (thymus) cells and monocytes .
There is increasing evidence for cytokine dysregulation in many chronic pulmonary diseases. In allergic asthma, IL-4 and IL-13 are produced by antigen-presenting cells, which lead to IgE production by B (bone marrow) cells and subsequent mast cell degranulation. In addition, eosinophils are stimulated by IL-5 that is produced by Th2 cells . The airway epithelium can also produce specific cytokines favoring Th2 and/or Th17 cell differentiation, such as IL-33 promoting IL-5 production and eosinophilia . Altogether, these secreted cytokines can initiate an asthmatic attack.
Many cytokines, including IL-1β and IL-6, have been shown to have a major role in COPD . Additionally, IL-1β levels were shown to be increased in the acute respiratory distress syndrome [1,32].
The following paragraphs will focus on interleukins and their roles in three major chronic inflammatory airway diseases including COPD, bronchiectasis, and allergic asthma.
COPD is currently the third leading cause of global mortality, according to the World Health Organization (WHO), and results in more than 120,000 deaths each year. Cigarette smoke is responsible for the majority of cases . COPD is characterized by progressive airway inflammation and acute exacerbations (AECOPD) due to infections, which are the major causes that lead to a significant increase in mortality. Chronic inflammation of the airways has been shown to play a key role in the pathophysiology of both COPD and AECOPD, which is associated with increased numbers of goblet cells, mucus gland hyperplasia, fibrosis and pulmonary emphysema . In this environment, activated macrophages release inflammatory mediators and chemotactic factors, such as tumor necrosis factor (TNF)-α, IL-6, IL-8, monocyte chemotactic peptide (MCP)-1, leukotriene (LT) B-4, and reactive oxygen species, which further promote airway destruction. Under the influence of IL-8 and leukotriene (LT)B-4 in particular, neutrophils migrate to the respiratory tract and cause mucus hypersecretion by submucosal glands and goblet cells [35,36,37,38]. Dendritic cells have also been shown to play a key role in activating a variety of inflammatory and immune cells including macrophages, B and T lymphocytes, and neutrophils in COPD [39,40,41].
The respiratory epithelium has been shown to secrete IL-1β and IL-8, which contribute to the development of small airways fibrosis [42,43,44]. Furthermore, IL-4 and -13 levels were shown to be elevated in central airways of smokers with chronic bronchitis, when compared to those of asymptomatic smokers . However, Imaoka and coauthors assessed serum levels of IL-4 and IL-13 in lungs of patients with severe COPD, smokers, and nonsmokers and could not find significant differences among these groups .
IL-18, a pro-inflammatory cytokine, has been shown to be produced intracellularly by caspase-1 cleavage from a biologically inactivated precursor, pro-IL-18, and secreted by activated macrophages . IL-18 proteins are expressed in the lungs of healthy individuals at low levels  serving as a co-factor for both Th1 and Th2 cell development [49,50,51]. Serum levels of IL-18 in COPD patients and smokers have been demonstrated to be elevated and negatively associated with the predicted forced expiratory volume in one second (FEV1), when compared to nonsmokers. The highest serum levels were found in patients with COPD stages III and IV according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) . In summary, these studies demonstrate that expression and secretion of various interleukins are altered in COPD, but further studies are needed to define their pathophysiological roles.
Bronchiectasis is an incurable pulmonary disorder, which is either a local or diffuse process affecting the bronchi . Sometimes described as a disease, bronchiectasis can be more appropriately thought of as a terminal pathological result of continuous airway inflammation, recurrent infection, and bronchial wall destruction, due to various primary causes [53,54]. Bronchiectasis was always thought to be a rare disorder but recent reports suggest that bronchiectasis is much more common, with an estimation of 139 cases per 100,000 persons, and its incidence and prevalence are rising within the aging population . Numerous causes can lead to the development of bronchiectasis, with post-infection bronchiectasis being the most common . Among the genetic diseases, cystic fibrosis (CF) is the most common cause; therefore, bronchiectasis has been divided into two groups: CF and non-CF bronchiectasis.
Controlled inflammation is essential for fighting infection; however, when in excess, it will lead to destruction of the lung and host cells . The bronchiectatic airway epithelium can produce an exaggerated pro-inflammatory cytokine response due to infectious stimuli . Therefore, the characterization of relevant inflammatory mediators is important for the development of more specific therapeutic approaches to control inflammation and improve health outcomes in individuals suffering from bronchiectasis .
CF has been used as a model disease to study the pathomechanisms leading to bronchiectasis. Interestingly, the CF airways are considered normal at birth, with the exception of some plugging of submucosal gland ducts . Since affected infants already show signs of inflammation and bacterial infection, especially with Staphylococcus aureus soon after diagnosis, lung disease begins early in life . Infants with normal lung function and no apparent bacterial colonization have already-high concentrations of inflammatory markers, such as interleukin 8 (IL-8), peroxidases, and their oxidants in bronchoalveolar lavage (BAL) fluid . The presence of respiratory symptoms has also been associated with elevated neutrophil count despite no evidence of infection . These findings have been controversial, since others suggest infection preceding inflammation in infants with CF . In addition, neutrophils are key inflammatory mediator cells in CF airways and contribute to tissue damage and disease progression. They secrete numerous different mediators that are critical for the neutrophil immune response. Unfortunately, a significant amount of those mediators leak from neutrophils during apoptosis and phagocytosis leading to further airway destruction .
2.3. Allergic Asthma
Allergic asthma is a chronic inflammatory airway disease, which occurs in response to inhaled allergens . It is characterized by reversible airway obstruction with airway hyperresponsiveness, submucosal infiltration of eosinophils and Th2 cells, mucus hypersecretion, and airway remodeling . Allergic asthma is a type 1 hypersensitivity reaction, which includes allergen-specific IgE molecules cross-linking to high-affinity Fcε receptors of basophils and mast cells. This leads to an immediate release of leukotrienes, prostaglandins, and histamines that induce contraction of airway smooth muscle cells, edema and mucus secretion leading to bronchoconstriction. Furthermore, locally produced chemokines recruit eosinophils, macrophages, neutrophils, and T lymphocytes . Cytokines are also released by eosinophils and epithelial cells, which ultimately initiate a chronic airway remodeling process, further recruitment of eosinophils, and stimulation and continued production of IgE by B cells [66,67]. IL-4, IL-5, IL-6, and IL-13 have been shown to maintain this cycle of allergic inflammation  with IL-4 promoting Ig isotype switching , IL-5 inducing eosinophil differentiation and migration , and IL-13 causing goblet cell hyperplasia and airway hyperresponsiveness [71,72]. IL-33 is another pro-inflammatory cytokine, which has been shown to drive neutrophil inflammation and promote airway remodeling in asthma [73,74].
2.4. Current Therapeutic Strategies and Their Effect on Airway Inflammation
With chronic airway inflammation and remodeling serving as key factors for the severity of asthma, COPD, and bronchiectasis, three classes of inhaled therapies have been utilized to aid in symptom management. Inhaled corticosteroids (ICSs) are commonly used in asthma to suppress airway inflammation and consequently reduce airway hyperresponsiveness . ICSs can often be combined with long acting β adrenergic receptor (B2) agonists (LABA) to help relieve symptoms of severe asthma . LABA and long acting muscarinic antagonists (LAMA) are bronchodilators commonly used for the symptomatic treatment of COPD. As a step-up therapy, LABAs and LAMAs are often given as a dual therapy in COPD patients to reduce bronchoconstriction, mucus secretion, and AECOPD [77,78]. The triple inhaled therapy is given to COPD patients who have continuing exacerbations or patients with severe persistent asthma. Although these inhaled therapies have been useful for their management of chronic airway inflammation, they also have their shortcomings. ICSs have high efficiency in treating asthma but are only partially effective in controlling airway inflammation. Moreover, ICSs have been linked to adverse cardiovascular effects in COPD patients, growth stunts in pediatric CF bronchiectasis patients, and withdrawal complications [75,79,80]. The biggest limitation of these inhaled therapies is their inability to aid in regulating disease modification and/or progression.
Regarding other anti-inflammatory therapy options, patients with CF bronchiectasis can use high dose ibuprofen. This is a recommended anti-inflammatory treatment, but mainly used in the pediatric population due to gastrointestinal side effects [76,78,80]. Azithromycin, an antibiotic with anti-inflammatory properties, has been shown to reduce exacerbation frequency in COPD patients when taken daily for one year . A similar effect of azithromycin use included less exacerbations, fewer positive cultures, less needed oral antibiotics, and significant improvements in lung function in CF patients, when taken chronically for at least six months . For COPD, phosphodiesterase 4 (PDE4) inhibitors have also emerged as an anti-inflammatory treatment option. A recent meta-analysis study showed that PDE4 inhibitors improved lung function and reduced AECOPD frequency, but they had little effect on quality of life or symptoms . Patients on this therapy frequently experienced gastrointestinal adverse effects and weight loss as well psychiatric adverse events. Therefore, current COPD treatment guidelines only support their use as an add-on therapy in patients with persistent symptoms or exacerbations despite optimal COPD management . Finally, theophylline (dimethylxanthine), one of the oldest medications, has been used to treat airway diseases. It is a bronchodilator but also shows anti-inflammatory effects via inhibition of PDE4 and histone deacetylase-2 activation . Its use, however, has decreased over the years mainly due to the high frequency of adverse effects even when used at conventional doses, such as headaches, nausea and vomiting, but also convulsions and cardiac arrhythmias .
Since COPD, CF, and non-CF bronchiectasis are associated with increased mucus secretion and impaired mucociliary clearance, mucus clearance therapies have been indicated and have shown improvement in both symptoms and chronic airway inflammation [86,87]. These therapies include not only a variety of airway clearance therapies (ACT)  but also the introduction of mucolytics, such as dornase alfa, hypertonic saline, and inhaled antibiotics. Airway clearance therapies are numerous but, so far, none have been shown to be superior. Therefore, the current recommendation is to perform ACT on a regular basis in all CF patients . Dornase alfa is a recombinant human deoxyribonuclease and has shown to facilitate mucus clearance in the lung, thereby preventing airway obstruction, exaggerated inflammation and, ultimately, lung function decline . Similarly, hyperosmolar saline has been used as an effective expectorant in patients with CF bronchiectasis. Current evidence only supports the use of hypertonic saline and dornase alfa in patients with CF . In summary, current established anti-inflammatory treatments in these chronic airway diseases are mainly symptomatic and broad in targeting multiple pro-inflammatory signaling pathways; therefore, their use is limited by various side effects.
3. Current Cytokine Specific Treatment Strategies
With the frequency and severity of airway inflammation in chronic lung disorders, a growing area of interest has been centered on the identification of factors that drive disease, including cytokines/chemokines, and their potential as viable therapeutic targets.
Th2 cytokines are the primary targets for biologic therapeutics for chronic airway diseases associated with eosinophilic inflammation. So far, anti-IL-5 therapy in asthma has been approved and cleared by the Food and Drug Administration (FDA), and clinical trials have been performed since 2000 with the first inhibitor, mepolizumab, which was approved for its use in 2015. Mepolizumab was shown to promote a reduction in eosinophils, acute exacerbations, and the need for steroids [91,92]. In recent years, two additional anti-IL-5 antibodies (reslizumab and benralizumab) have been approved by the FDA for their safety and efficacy in reducing asthma exacerbations [93,94]. IL-5 therapeutics are currently being studied for an indication in COPD; phase 3 trials are currently being conducted using benralizumab and mepolizumab. The first results showed that benralizumab displayed a mild beneficial effect on lung function, while treatment with mepolizumab reduced COPD exacerbations [95,96].
Similar to the IL-5 treatment strategies in COPD, an anti-IL-4 antibody has been studied in a clinical trial as an indication for asthma, and the IL-4 receptor α inhibitor, dupilumab, has shown notable improvements in exacerbations and lung function from asthmatic patients that are already on dual therapy with ICSs and LABA [97,98]. Recently, dupilumab was approved by the FDA for its asthma indication but has not been examined for its efficacy in COPD to date. The anti-IL-13 therapeutics lebrikizumab and tralokinumab were used in phase 3 clinical asthma trials; however, the inhibitors showed negligible to no effects on lung function or exacerbation reduction .
4. Future Therapeutic Strategies
Several pro-inflammatory cytokines have been shown to be upregulated and characterized as contributors to disease severity in asthma, COPD, and CF bronchiectasis making them appropriate therapeutic targets. These will be discussed in detail in the following paragraphs.
4.1. Inhibition of IL-1β as Therapeutic Strategy
IL-1β is a pro-inflammatory cytokine with increased expression in asthma, COPD, and CF bronchiectasis. Our group has demonstrated that the bronchial epithelium secretes IL-1β in response to cigarette smoke  leading to lung inflammation, emphysema, fibrosis, and goblet cell hyperplasia in mice . Pro-IL-1β, produced by activation of the nuclear factor (NF)-κB signaling cascade, is converted into its cytosolic active form by the inflammasome, which mediates the auto-activation of caspase-1, cleaving pro-IL-1β into its biologically active form [101,102].
Infection with Pseudomonas aeruginosa (PsA), one of the most clinically relevant pathogens in CF bronchiectasis, can lead to an increase in levels of IL-1β in BAL fluid from these patients [103,104]. In addition, polymorphisms in the IL1B gene have been shown to be associated with disease severity . Along these findings, Muselet-Charlier and coauthors found a rapid IL-1β mediated activation of NF-κB in a CF lung epithelial cell line . CF mice exhibited augmented IL-1β signaling in response to PsA, and PsA-mediated lung inflammation and bacterial load were attenuated by a neutralizing IL-1β antibody . In addition, dysfunction of the inflammasome, namely pyrin domain containing 3 (NLRP3) as a key activating factor, led to IL-1β-dependent inflammation in both murine and human CF bronchiectasis disease. This NLRP3 activity was shown to be regulated by IL-1 receptor antagonist (IL-1RA) in a negative feedback loop, thereby providing a potential therapeutic angle to attenuate CF airway disease by chronic colonization .
Altogether, these data highlight the involvement of IL-1β in smoke and CF-related inflammatory airway disease and IL-1β inhibition as potential future therapeutic application.
IL-1β has also been shown to be upregulated in neutrophilic asthma compared to eosinophilic and pauci-granulocytic asthma . He et al. conducted a meta-analysis summarizing 15 case-control studies and analyzed the association between asthma risk and genetic polymorphisms in IL-1β -511C/T and IL-1RA. No association was found for the IL-1β -511C/T polymorphism, but the IL-1RA polymorphism was related to an increased risk of asthma, which was independent of ethnicity and age . Furthermore, Besnard et al. concluded that inflammasome-induced IL-1β production ultimately contributes to the control of allergic asthma by enhancing Th17 cell differentiation . Another study along these lines could demonstrate that the IL-1 receptor antagonist and IL-1 type-II receptor attenuated both IL-5- and IgE-mediated changes in airway smooth muscle cell responsiveness. Human airway smooth muscle cells, exposed to IL-5, IL-1β and IgE, upregulated expression levels of both stimulatory and inhibitory IL-1 axis molecules, which suggests that modulation of the interleukin-1 axis may potentially also have significant therapeutic implications in the treatment of asthma .
So far, small clinical trials have been performed examining the role of IL-β blockade for asthma and COPD. Canakinumab is a high-affinity human immunoglobulin G kappa (IgGk) monoclonal antibody that targets Il-1β by neutralizing its bioactivity. One randomized double-blinded trial in asthmatic patients has been conducted so far, which consisted of two single administrations on day 1 and day 15 in patients with mild asthma. Patients were allowed to stay on other anti-asthmatic drugs and allergen challenge was performed on day 0 and day 28. The results showed that canakinumab led to a 28% decrease in the late asthmatic response. Furthermore, a single dose of canakinumab significantly reduced circulating IL-1β levels for the time measured. Although this trial was small and included only 16 patients, the results were positive and promising .
The impact of canakinumab on pulmonary function in COPD was also assessed in a phase 1/2 study, which included 147 participants. Individuals received either drug or placebo intravenous infusion at weeks 1, 5, 7, and thereafter every 4 weeks for a total of 45 weeks. The primary outcome measure did not show any significant difference in lung function between groups. Is this study alone sufficient to “disqualify” canakinumab, or were the studied outcome measures just not sensitive enough? Should the study have been conducted for a longer time and should COPD stages, progression, or COPD-associated inflammation have been assessed instead? These are all valid questions and may have contributed to a different outcome; therefore, this study alone should not preclude the use of canakinumab as a potential future therapy in COPD.
Anakinra is a recombinant IL-1ra protein that can block IL-1β mediated effects and therefore, represents an attractive novel therapy for chronic inflammatory airway diseases. Hernandez et al. conducted a small study to assess the effect of anakinra on the acute neutrophil response after an inhaled endotoxin challenge in 17 healthy volunteers. The authors could show that anakinra effectively reduced neutrophilic airway inflammation without any serious adverse effects, thus making anakinra a potential target for the treatment of asthma with neutrophil predominance . A follow up phase 1/2 trial is currently enrolling patients with mild allergic asthma to test the efficacy of anakinra and its therapeutic role in allergic asthma. Furthermore, both anakinra and canakinumab could have potential therapeutic anti-inflammatory implications in bronchiectatic lung diseases. Recent reports have identified the IL-1 receptor for its critical role in the pathogenesis of a murine CF model, in which anakinra was able to efficiently block CF-related airway inflammation and mucus obstruction .
Nevertheless, caution should be taken when using IL-1 inhibitory therapy due to the primary role of IL-1β activating the inflammasome and protecting against respiratory infections. IL-1 inhibition may render patients with chronic inflammatory respiratory disorders more susceptible to active airway infection and exacerbations. Therefore, targeted approaches to suppress the inflammatory response need to be assessed thoroughly and used wisely for certain subgroups of patients.
4.2. IL-6 Blocking Antibody Therapy
IL-6 can be produced by both inflammatory and primary lung epithelial cells in response to a variety of different stimuli [116,117,118]. The age-dependent onset of frailty, loss of lung function, and increased prevalence of COPD suggests involvement of aging related mechanisms or “inflammaging”. One concept characterizes aging as a proinflammatory condition due to the underlying immune dysfunction with COPD. Therefore, emphysema development has been shown to represent an “accelerated aging phenotype” [119,120]. Aging-related pro-inflammatory cytokines, especially IL-6, have been linked to negative health outcomes and mortality, and speculated to result in persistent, low grade activation of chronic inflammation . Other explanations for this aging-associated rise in circulating IL-6 levels include increased oxidative stress or persistent viral infections (herpes or cytomegalovirus) as observed in the frail and elderly [122,123]. Characterizing the mechanism of IL-6 in affecting frailty, lung disease (specifically COPD), and adverse outcomes may prove to be an important field of future research. In CF bronchiectasis, Nixon and co-authors demonstrated that IL-6 sputum levels were negatively associated with FEV1 and forced vital capacity (FVC) with IL-6 decreasing after antibiotic treatment .
Analyzing the role of IL-6 in asthma revealed that IL-6 mRNA was constitutively present in mouse primary lung epithelial cells, but not in resident immune cells. Interestingly, IL-6-induced IL-4 production during Th2 differentiation has been shown to inhibit Th1 differentiation  and, together with transforming growth factor (TGF)-β, IL-6 has also been documented to promote Th17 cell differentiation [126,127]. Asthmatic patients (including allergic and intrinsic asthma) were shown to have increased IL-6 serum and BAL fluid levels [118,128,129]. Two studies showed that the levels of IL-6 in sputum of asthmatic patients inversely correlated with FEV1 [130,131]. This also held true in obese asthma patients .
These studies indicate that IL-6 seems to be directly involved in both the pathogenesis of asthma as well as its associated progressive loss of lung function.
Blocking antibodies to IL-6 and its receptor have been studied in other inflammatory diseases, such as rheumatoid arthritis and Crohn’s disease. Clinical trials for tocilizumab, a blocking antibody for the IL-6 receptor, revealed decreased IL-6 levels, improved disease activity scores, and symptoms in rheumatoid arthritis. The antibody was approved in 2010 but has not been tested in chronic inflammatory airway diseases to date . Therefore, targeting IL-6 could be a potential novel and promising treatment strategy to suppress not only chronic airway but also its associated systemic inflammation, COPD-associated frailty and decline in lung function.
4.3. Inhibition of IL-8 Signaling as an Anti-Inflammatory Therapy Approach
IL-8 is an essential cytokine in neutrophil recruitment and the inflamed bronchial epithelium is a major source of IL-8 [134,135]. We have shown that increases in TGF-β and fibroblast growth factor (FGF) 23 led to increased bronchial epithelial IL-8 secretion . In addition, NF-κb has been shown to mediate IL-8 expression in CF bronchiectasis with an upregulation in early phases of PsA colonization [106,136,137]. IL-1β also induced IL-8 in the bronchial epithelium of CF patients [101,138]. Other reports, however, demonstrated no differences in IL-8 secretion between CF and control airway epithelial cell cultures, but increased IL-8 secretion was observed after infection with PsA .
IL-8 has also been characterized as a key contributor to the development of COPD; the COPD bronchial epithelium has been shown to have a higher baseline expression of IL-8, thus leading directly to mucus hypersecretion by induction of the mucin genes MUC5AC and MUC5B [140,141,142]. Both cigarette smoke and tumor necrosis factor (TNF)-α have been shown to synergistically stimulate macrophage IL-8 production . This suggests that cigarette smoke is a sufficient stimulator for excessive IL-8 release in COPD airways . Furthermore, neutrophils and circulating IL-8 have been shown to accumulate in AECOPD [145,146], with IL-8 being negatively associated with lung function .
One study in asthmatic patients assessed sputum concentrations of IL-8, demonstrating increased levels in severe versus mild asthma . Another report showed that IL-8 in tracheal aspirates was significantly higher in patients who were intubated for acute severe asthma, when compared to control individuals without lung disease . This finding was consistent with a previous report demonstrating a marked increase in IL-8 in BAL fluid from patients with status asthmaticus . Hosoki et al. identified neutrophils and IL-8 as the only inflammatory components in BAL fluids that distinguished controlled asthma from uncontrolled asthma with an inverse correlation to FEV1 . In summary, these studies suggest that IL-8 is an essential pro-inflammatory mediator in asthma and a possible prognostic biomarker for disease severity.
So far, the effects of blocking IL-8 have been examined in COPD; however, no significant clinical improvements have been detected in patients in these small trials . Interestingly, the chemokine receptor C-X-C chemokine receptor (CXCR) 2 is a chemo attractant for neutrophils and has been shown to aid in the production of IL-8. Its antagonist has been shown to decrease the levels of IL-8 production making it a clinical target. Treatment with the CXCR2 receptor antagonist, navarinxin, has shown a significant reduction of sputum and blood neutrophils in asthmatic patients without any effect on lung function . In clinical COPD trials, navarinxin caused small improvements in lung function . An additional CXCR2 antagonist, danirixin, is currently under investigation for clinical usage . SB-656933, a receptor antagonist for CXCR2, has been tested in patients with CF bronchiectasis and was shown to be well-tolerated (except minor reports of headaches) with improvements in sputum neutrophils but no improvement in symptoms or lung function . Therefore, these therapies are promising but will need further evaluation and testing.
The diagram below is summarizing the three chronic inflammatory airway diseases discussed in this review and the involvement of different cell types, pro- and anti-inflammatory cytokines and current treatment strategies (Figure 1).
4.4. Future Approaches Targeting IL-17 and IL-23
IL-17 plays an essential role in the pulmonary host defense by inducing neutrophil local release of neutrophil-mobilizing factors, including CXC chemokines . Recent reports are controversial regarding the role of IL-17 in the development of COPD [157,158,159,160]; IL-17 levels were shown to be low in the sputum of individuals with COPD , and there was no difference in IL-17 receptor expression levels between individuals with COPD and non-COPD lung disease . However, IL-17 was also shown to activate neutrophils, thereby enhancing epithelial production of IL-8 and MUC5AC synthesis in the bronchial epithelium [163,164]. Taking these results together, IL-17 is most likely part of the protective immunity in COPD.
In bronchiectatic airways, IL-17 was detectable in bronchoalveolar lavage fluid and led to increased expression of MUC5AC and MUC5B. Treatment of epithelial cultures with IL-17 caused an increase of IL-8, thereby contributing to airway inflammation . Hsu and coauthors showed that mice, infected with PsA, had a significant upregulation of IL-17 that was associated with a persistent inflammatory response. A similar response could be mimicked by direct intratracheal administration of IL-17 with attenuation by neutralization of IL-17 .
Similarly to the COPD data, Th17 cells and their corresponding cytokines, IL-17A and IL-17F, appeared to contribute to the pathogenesis of primarily severe and steroid-resistant asthma . The asthmatic airway epithelium also produced specific cytokines favoring Th2 and/or Th17 cell differentiation, such as IL-33-promoting IL-5 production and eosinophilia .
Due to the evidence presented here, modulation of IL-17 is an attractive potential therapeutic target in chronic inflammatory airway diseases [167,168,169]. Small clinical trials have examined the effectiveness of IL-17 and its receptor antagonist in asthma and COPD. Unfortunately, no improvements in lung function or disease symptoms have been observed in patients with either disease [170,171]. Risankizumab, an anti-IL-23 antibody, is in the early stages of clinical trials for severe asthma . Although the three cytokines are not always regulated synergistically, regulation of the Th17 cytokine axis (IL-23, IL-17A, and IL-22) might prove to be beneficial for several inflammatory diseases.
Below is a diagram summarizing current and potential future cytokine-targeted therapies in chronic inflammatory airway diseases (Figure 2).
Managing chronic lung disorders and chronic airway inflammation are global concerns, and their prevalence is increasing due to an aging population and a substantial increase of particulate matter air pollution over recent decades. Developing a better understanding of pro-and anti-inflammatory cytokines that regulate and/or drive disease severity serves as a beneficial tool in the development of modern therapeutics. Even though extensive progress has been made thus far, most of our therapeutic approaches for chronic inflammatory airway diseases target broadly with significant side effects. Recent attempts of specific anti-cytokine therapies have only shown some benefits. Future research is needed to help find novel and more specific targets, or for more in-depth evaluation of the specific therapies we already have. In addition, the right patient subgroups and timing for initiation of treatment need to be thoroughly investigated, since inflammation is needed to activate a proper immune response. This might be the explanation for some of the negative results of current clinical anti-cytokine trials. Therefore, it is worth conducting long-term studies with anakinra and canakinumab, if lung function or disease progression is an outcome measure. Furthermore, other outcome measures might be more appropriate when assessing anti-inflammatory treatments, such as exacerbation or hospitalization frequency. Hopefully, further research will help in the future to not only identify novel treatments for chronic inflammatory lung disorders but also aid in a better understanding of their pathophysiology.
This work was supported by the Flight Attendant Medical Research Institute (YFAC152003 to S.K.) and the Cystic Fibrosis Foundation (CFF KRICK1610 to S.K.) and the NIH (K99HL131866/R00HL131866 to J.B. and T32 HL007457 to J.G.).
Conflicts of Interest
The authors declare no conflict of interest.
- Murray, C.J.; Vos, T.; Lozano, R.; Naghavi, M.; Flaxman, A.D.; Michaud, C.; Ezzati, M.; Shibuya, K.; Salomon, J.A.; Abdalla, S.; et al. Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990–2010: A systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012, 380, 2197–2223. [Google Scholar] [CrossRef]
- Ozdoganoglu, T.; Songu, M. The burden of allergic rhinitis and asthma. Ther. Adv. Respir. Dis. 2012, 6, 11–23. [Google Scholar] [CrossRef] [PubMed]
- Nakata, K.; Gotoh, H.; Watanabe, J.; Uetake, T.; Komuro, I.; Yuasa, K.; Watanabe, S.; Ieki, R.; Sakamaki, H.; Akiyama, H.; et al. Augmented proliferation of human alveolar macrophages after allogeneic bone marrow transplantation. Blood 1999, 93, 667–673. [Google Scholar] [PubMed]
- Bender, A.T.; Ostenson, C.L.; Wang, E.H.; Beavo, J.A. Selective up-regulation of PDE1B2 upon monocyte-to-macrophage differentiation. Proc. Natl. Acad. Sci. USA 2005, 102, 497–502. [Google Scholar] [CrossRef] [PubMed]
- Burns, A.R.; Smith, C.W.; Walker, D.C. Unique structural features that influence neutrophil emigration into the lung. Physiol. Rev. 2003, 83, 309–336. [Google Scholar] [CrossRef] [PubMed]
- Yazdanbakhsh, M.; Kremsner, P.G.; van Ree, R. Allergy, parasites, and the hygiene hypothesis. Science 2002, 296, 490–494. [Google Scholar] [CrossRef] [PubMed]
- Tesmer, L.A.; Lundy, S.K.; Sarkar, S.; Fox, D.A. Th17 cells in human disease. Immunol. Rev. 2008, 223, 87–113. [Google Scholar] [CrossRef] [PubMed]
- Adler, K.B.; Fischer, B.M.; Wright, D.T.; Cohn, L.A.; Becker, S. Interactions between respiratory epithelial cells and cytokines: Relationships to lung inflammation. Ann. N. Y. Acad. Sci. 1994, 725, 128–145. [Google Scholar] [CrossRef] [PubMed]
- Suratt, B.T.; Parsons, P.E. Mechanisms of acute lung injury/acute respiratory distress syndrome. Clin. Chest Med. 2006, 27, 579–589. [Google Scholar] [CrossRef] [PubMed]
- Hallstrand, T.S.; Hackett, T.L.; Altemeier, W.A.; Matute-Bello, G.; Hansbro, P.M.; Knight, D.A. Airway epithelial regulation of pulmonary immune homeostasis and inflammation. Clin. Immunol. 2014, 151, 1–15. [Google Scholar] [CrossRef] [PubMed]
- Ince, L.M.; Zhang, Z.; Beesley, S.; Vonslow, R.M.; Saer, B.R.; Matthews, L.C.; Begley, N.; Gibbs, J.E.; Ray, D.W.; Loudon, A.S.I. Circadian variation in pulmonary inflammatory responses is independent of rhythmic glucocorticoid signaling in airway epithelial cells. FASEB J. 2018. [Google Scholar] [CrossRef] [PubMed]
- Krick, S.; Grabner, A.; Baumlin, N.; Yanucil, C.; Helton, S.; Grosche, A.; Sailland, J.; Geraghty, P.; Viera, L.; Russell, D.W.; et al. Fibroblast growth factor 23 and Klotho contribute to airway inflammation. Eur. Respir. J. 2018, 52, 1800236. [Google Scholar] [CrossRef] [PubMed]
- Krick, S.; Baumlin, N.; Aller, S.P.; Aguiar, C.; Grabner, A.; Sailland, J.; Mendes, E.; Schmid, A.; Qi, L.; David, N.V.; et al. Klotho Inhibits Interleukin-8 Secretion from Cystic Fibrosis Airway Epithelia. Sci. Rep. 2017, 7, 14388. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Parker, D.; Prince, A. Type I interferon response to extracellular bacteria in the airway epithelium. Trends Immunol. 2011, 32, 582–588. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Gras, D.; Chanez, P.; Vachier, I.; Petit, A.; Bourdin, A. Bronchial epithelium as a target for innovative treatments in asthma. Pharmacol. Ther. 2013, 140, 290–305. [Google Scholar] [CrossRef] [PubMed]
- Knight, D.A.; Holgate, S.T. The airway epithelium: Structural and functional properties in health and disease. Respirology 2003, 8, 432–446. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Crystal, R.G.; Randell, S.H.; Engelhardt, J.F.; Voynow, J.; Sunday, M.E. Airway epithelial cells: Current concepts and challenges. Proc. Am. Thorac. Soc. 2008, 5, 772–777. [Google Scholar] [CrossRef] [PubMed]
- Suzuki, K.; Hori, A.; Kawamoto, K.; Thangudu, R.R.; Ishida, T.; Igarashi, K.; Samejima, M.; Yamada, C.; Arakawa, T.; Wakagi, T.; et al. Crystal structure of a feruloyl esterase belonging to the tannase family: A disulfide bond near a catalytic triad. Proteins 2014, 82, 2857–2867. [Google Scholar] [CrossRef] [PubMed]
- Widdicombe, J.H.; Widdicombe, J.G. Regulation of human airway surface liquid. Respir. Physiol. 1995, 99, 3–12. [Google Scholar] [CrossRef]
- Corfield, A.P. Mucins: A biologically relevant glycan barrier in mucosal protection. Biochim. Biophys. Acta 2015, 1850, 236–252. [Google Scholar] [CrossRef] [PubMed]
- Cole, A.M.; Liao, H.I.; Stuchlik, O.; Tilan, J.; Pohl, J.; Ganz, T. Cationic polypeptides are required for antibacterial activity of human airway fluid. J. Immunol. 2002, 169, 6985–6991. [Google Scholar] [CrossRef] [PubMed]
- Perez-Novo, C.A.; Watelet, J.B.; Claeys, C.; Van Cauwenberge, P.; Bachert, C. Prostaglandin, leukotriene, and lipoxin balance in chronic rhinosinusitis with and without nasal polyposis. J. Allergy Clin. Immunol. 2005, 115, 1189–1196. [Google Scholar] [CrossRef] [PubMed]
- Gohy, S.T.; Hupin, C.; Pilette, C.; Ladjemi, M.Z. Chronic inflammatory airway diseases: The central role of the epithelium revisited. Clin. Exp. Allergy 2016, 46, 529–542. [Google Scholar] [CrossRef] [PubMed]
- Gao, W.; Li, L.; Wang, Y.; Zhang, S.; Adcock, I.M.; Barnes, P.J.; Huang, M.; Yao, X. Bronchial epithelial cells: The key effector cells in the pathogenesis of chronic obstructive pulmonary disease? Respirology 2015, 20, 722–729. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Moldoveanu, B.; Otmishi, P.; Jani, P.; Walker, J.; Sarmiento, X.; Guardiola, J.; Saad, M.; Yu, J. Inflammatory mechanisms in the lung. J. Inflamm. Res. 2009, 2, 1–11. [Google Scholar] [PubMed]
- Toossi, Z.; Hirsch, C.S.; Hamilton, B.D.; Knuth, C.K.; Friedlander, M.A.; Rich, E.A. Decreased production of TGF-beta 1 by human alveolar macrophages compared with blood monocytes. J. Immunol. 1996, 156, 3461–3468. [Google Scholar] [PubMed]
- Moore, S.A.; Strieter, R.M.; Rolfe, M.W.; Standiford, T.J.; Burdick, M.D.; Kunkel, S.L. Expression and regulation of human alveolar macrophage-derived interleukin-1 receptor antagonist. Am. J. Respir. Cell Mol. Biol. 1992, 6, 569–575. [Google Scholar] [CrossRef] [PubMed]
- Ding, L.; Linsley, P.S.; Huang, L.Y.; Germain, R.N.; Shevach, E.M. IL-10 inhibits macrophage costimulatory activity by selectively inhibiting the up-regulation of B7 expression. J. Immunol. 1993, 151, 1224–1234. [Google Scholar] [PubMed]
- Holgate, S.T. Pathogenesis of asthma. Clin. Exp. Allergy 2008, 38, 872–897. [Google Scholar] [CrossRef] [PubMed]
- Kurowska-Stolarska, M.; Kewin, P.; Murphy, G.; Russo, R.C.; Stolarski, B.; Garcia, C.C.; Komai-Koma, M.; Pitman, N.; Li, Y.; Niedbala, W.; et al. IL-33 induces antigen-specific IL-5+ T cells and promotes allergic-induced airway inflammation independent of IL-4. J. Immunol. 2008, 181, 4780–4790. [Google Scholar] [CrossRef] [PubMed]
- Sarir, H.; Henricks, P.A.; van Houwelingen, A.H.; Nijkamp, F.P.; Folkerts, G. Cells, mediators and Toll-like receptors in COPD. Eur. J. Pharmacol. 2008, 585, 346–353. [Google Scholar] [CrossRef] [PubMed]
- Suter, P.M.; Suter, S.; Girardin, E.; Roux-Lombard, P.; Grau, G.E.; Dayer, J.M. High bronchoalveolar levels of tumor necrosis factor and its inhibitors, interleukin-1, interferon, and elastase, in patients with adult respiratory distress syndrome after trauma, shock, or sepsis. Am. Rev. Respir. Dis. 1992, 145, 1016–1022. [Google Scholar] [CrossRef] [PubMed]
- Samet, J.M. Tobacco smoking: The leading cause of preventable disease worldwide. Thorac. Surg. Clin. 2013, 23, 103–112. [Google Scholar] [CrossRef] [PubMed]
- McDonough, J.E.; Yuan, R.; Suzuki, M.; Seyednejad, N.; Elliott, W.M.; Sanchez, P.G.; Wright, A.C.; Gefter, W.B.; Litzky, L.; Coxson, H.O.; et al. Small-airway obstruction and emphysema in chronic obstructive pulmonary disease. N. Engl. J. Med. 2011, 365, 1567–1575. [Google Scholar] [CrossRef] [PubMed]
- Barnes, P.J.; Shapiro, S.D.; Pauwels, R.A. Chronic obstructive pulmonary disease: Molecular and cellular mechanisms. Eur. Respir. J. 2003, 22, 672–688. [Google Scholar] [CrossRef] [PubMed]
- Barnes, P.J. Mechanisms in COPD: Differences from asthma. Chest 2000, 117 (Suppl. 2), 10S–14S. [Google Scholar] [CrossRef] [PubMed]
- Bucchioni, E.; Kharitonov, S.A.; Allegra, L.; Barnes, P.J. High levels of interleukin-6 in the exhaled breath condensate of patients with COPD. Respir. Med. 2003, 97, 1299–1302. [Google Scholar] [CrossRef] [PubMed]
- Sommerhoff, C.P.; Nadel, J.A.; Basbaum, C.B.; Caughey, G.H. Neutrophil elastase and cathepsin G stimulate secretion from cultured bovine airway gland serous cells. J. Clin. Investig. 1990, 85, 682–689. [Google Scholar] [CrossRef] [PubMed]
- Retamales, I.; Elliott, W.M.; Meshi, B.; Coxson, H.O.; Pare, P.D.; Sciurba, F.C.; Rogers, R.M.; Hayashi, S.; Hogg, J.C. Amplification of inflammation in emphysema and its association with latent adenoviral infection. Am. J. Respir. Crit. Care Med. 2001, 164, 469–473. [Google Scholar] [CrossRef] [PubMed]
- Finkelstein, R.; Fraser, R.S.; Ghezzo, H.; Cosio, M.G. Alveolar inflammation and its relation to emphysema in smokers. Am. J. Respir. Crit. Care Med. 1995, 152 Pt 1, 1666–1672. [Google Scholar] [CrossRef]
- Banchereau, J.; Briere, F.; Caux, C.; Davoust, J.; Lebecque, S.; Liu, Y.J.; Pulendran, B.; Palucka, K. Immunobiology of dendritic cells. Annu. Rev. Immunol. 2000, 18, 767–811. [Google Scholar] [CrossRef] [PubMed]
- Mio, T.; Romberger, D.J.; Thompson, A.B.; Robbins, R.A.; Heires, A.; Rennard, S.I. Cigarette smoke induces interleukin-8 release from human bronchial epithelial cells. Am. J. Respir. Crit. Care Med. 1997, 155, 1770–1776. [Google Scholar] [CrossRef] [PubMed]
- Hellermann, G.R.; Nagy, S.B.; Kong, X.; Lockey, R.F.; Mohapatra, S.S. Mechanism of cigarette smoke condensate-induced acute inflammatory response in human bronchial epithelial cells. Respir. Res. 2002, 3, 22. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Floreani, A.A.; Wyatt, T.A.; Stoner, J.; Sanderson, S.D.; Thompson, E.G.; Allen-Gipson, D.; Heires, A.J. Smoke and C5a induce airway epithelial intercellular adhesion molecule-1 and cell adhesion. Am. J. Respir. Cell Mol. Biol. 2003, 29, 472–482. [Google Scholar] [CrossRef] [PubMed]
- Miotto, D.; Ruggieri, M.P.; Boschetto, P.; Cavallesco, G.; Papi, A.; Bononi, I.; Piola, C.; Murer, B.; Fabbri, L.M.; Mapp, C.E. Interleukin-13 and -4 expression in the central airways of smokers with chronic bronchitis. Eur. Respir. J. 2003, 22, 602–608. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Imaoka, H.; Hoshino, T.; Takei, S.; Kinoshita, T.; Okamoto, M.; Kawayama, T.; Kato, S.; Iwasaki, H.; Watanabe, K.; Aizawa, H. Interleukin-18 production and pulmonary function in COPD. Eur. Respir. J. 2008, 31, 287–297. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Nakanishi, K.; Yoshimoto, T.; Tsutsui, H.; Okamura, H. Interleukin-18 regulates both Th1 and Th2 responses. Annu. Rev. Immunol. 2001, 19, 423–474. [Google Scholar] [CrossRef] [PubMed]
- Kitasato, Y.; Hoshino, T.; Okamoto, M.; Kato, S.; Koda, Y.; Nagata, N.; Kinoshita, M.; Koga, H.; Yoon, D.Y.; Asao, H.; et al. Enhanced expression of interleukin-18 and its receptor in idiopathic pulmonary fibrosis. Am. J. Respir. Cell Mol. Biol. 2004, 31, 619–625. [Google Scholar] [CrossRef] [PubMed]
- Hoshino, T.; Kato, S.; Oka, N.; Imaoka, H.; Kinoshita, T.; Takei, S.; Kitasato, Y.; Kawayama, T.; Imaizumi, T.; Yamada, K.; et al. Pulmonary inflammation and emphysema: Role of the cytokines IL-18 and IL-13. Am. J. Respir. Crit. Care Med. 2007, 176, 49–62. [Google Scholar] [CrossRef] [PubMed]
- Hoshino, T.; Yagita, H.; Ortaldo, J.R.; Wiltrout, R.H.; Young, H.A. In vivo administration of IL-18 can induce IgE production through Th2 cytokine induction and up-regulation of CD40 ligand (CD154) expression on CD4+ T cells. Eur. J. Immunol. 2000, 30, 1998–2006. [Google Scholar] [CrossRef][Green Version]
- Hoshino, T.; Wiltrout, R.H.; Young, H.A. IL-18 is a potent coinducer of IL-13 in NK and T cells: A new potential role for IL-18 in modulating the immune response. J. Immunol. 1999, 162, 5070–5077. [Google Scholar] [PubMed]
- Barker, A.F. Bronchiectasis. N. Engl. J. Med. 2002, 346, 1383–1393. [Google Scholar] [CrossRef] [PubMed]
- Chalmers, J.D.; Aliberti, S.; Blasi, F. Management of bronchiectasis in adults. Eur. Respir. J. 2015, 45, 1446–1462. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Aksamit, T.R.; O’Donnell, A.E.; Barker, A.; Olivier, K.N.; Winthrop, K.L.; Daniels, M.L.A.; Johnson, M.; Eden, E.; Griffith, D.; Knowles, M.; et al. Adult Patients With Bronchiectasis: A First Look at the US Bronchiectasis Research Registry. Chest 2017, 151, 982–992. [Google Scholar] [CrossRef] [PubMed]
- Weycker, D.; Hansen, G.L.; Seifer, F.D. Prevalence and incidence of noncystic fibrosis bronchiectasis among US adults in 2013. Chronic Respir. Dis. 2017, 14, 377–384. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Sidhu, M.K.; Mandal, P.; Hill, A.T. Bronchiectasis: An update on current pharmacotherapy and future perspectives. Expert Opin. Pharmacother. 2014, 15, 505–525. [Google Scholar] [CrossRef] [PubMed]
- Hamutcu, R.; Rowland, J.M.; Horn, M.V.; Kaminsky, C.; MacLaughlin, E.F.; Starnes, V.A.; Woo, M.S. Clinical findings and lung pathology in children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 2002, 165, 1172–1175. [Google Scholar] [CrossRef] [PubMed]
- Venkatakrishnan, A.; Stecenko, A.A.; King, G.; Blackwell, T.R.; Brigham, K.L.; Christman, J.W.; Blackwell, T.S. Exaggerated activation of nuclear factor-kappaB and altered IkappaB-beta processing in cystic fibrosis bronchial epithelial cells. Am. J. Respir. Cell Mol. Biol. 2000, 23, 396–403. [Google Scholar] [CrossRef] [PubMed]
- Koehler, D.R.; Downey, G.P.; Sweezey, N.B.; Tanswell, A.K.; Hu, J. Lung inflammation as a therapeutic target in cystic fibrosis. Am. J. Respir. Cell Mol. Biol. 2004, 31, 377–381. [Google Scholar] [CrossRef] [PubMed]
- Verhaeghe, C.; Delbecque, K.; de Leval, L.; Oury, C.; Bours, V. Early inflammation in the airways of a cystic fibrosis foetus. J. Cyst. Fibros. 2007, 6, 304–308. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Khan, T.Z.; Wagener, J.S.; Bost, T.; Martinez, J.; Accurso, F.J.; Riches, D.W. Early pulmonary inflammation in infants with cystic fibrosis. Am. J. Respir. Crit. Care Med. 1995, 151, 1075–1082. [Google Scholar] [PubMed]
- Sly, P.D.; Brennan, S.; Gangell, C.; de Klerk, N.; Murray, C.; Mott, L.; Stick, S.M.; Robinson, P.J.; Robertson, C.F.; Ranganathan, S.C.; et al. Lung disease at diagnosis in infants with cystic fibrosis detected by newborn screening. Am. J. Respir. Crit. Care Med. 2009, 180, 146–152. [Google Scholar] [CrossRef] [PubMed]
- Armstrong, D.S.; Hook, S.M.; Jamsen, K.M.; Nixon, G.M.; Carzino, R.; Carlin, J.B.; Robertson, C.F.; Grimwood, K. Lower airway inflammation in infants with cystic fibrosis detected by newborn screening. Pediatr. Pulmonol. 2005, 40, 500–510. [Google Scholar] [CrossRef] [PubMed]
- Sagel, S.D.; Wagner, B.D.; Anthony, M.M.; Emmett, P.; Zemanick, E.T. Sputum biomarkers of inflammation and lung function decline in children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 2012, 186, 857–865. [Google Scholar] [CrossRef] [PubMed]
- Rincon, M.; Irvin, C.G. Role of IL-6 in asthma and other inflammatory pulmonary diseases. Int. J. Biol. Sci. 2012, 8, 1281–1290. [Google Scholar] [CrossRef] [PubMed]
- Broide, D.H. Molecular and cellular mechanisms of allergic disease. J. Allergy Clin. Immunol. 2001, 108 (Suppl. 2), S65–S71. [Google Scholar] [CrossRef] [PubMed]
- Vignola, A.M.; Kips, J.; Bousquet, J. Tissue remodeling as a feature of persistent asthma. J. Allergy Clin. Immunol. 2000, 105, 1041–1053. [Google Scholar] [CrossRef] [PubMed]
- Agrawal, D.K.; Shao, Z. Pathogenesis of allergic airway inflammation. Curr. Allergy Asthma Rep. 2010, 10, 39–48. [Google Scholar] [CrossRef] [PubMed]
- Berton, M.T.; Uhr, J.W.; Vitetta, E.S. Synthesis of germ-line gamma 1 immunoglobulin heavy-chain transcripts in resting B cells: Induction by interleukin 4 and inhibition by interferon gamma. Proc. Natl. Acad. Sci. USA 1989, 86, 2829–2833. [Google Scholar] [CrossRef] [PubMed]
- Wills-Karp, M. Immunologic basis of antigen-induced airway hyperresponsiveness. Annu. Rev. Immunol. 1999, 17, 255–281. [Google Scholar] [CrossRef] [PubMed]
- Whittaker, L.; Niu, N.; Temann, U.A.; Stoddard, A.; Flavell, R.A.; Ray, A.; Homer, R.J.; Cohn, L. Interleukin-13 mediates a fundamental pathway for airway epithelial mucus induced by CD4 T cells and interleukin-9. Am. J. Respir. Cell Mol. Biol. 2002, 27, 593–602. [Google Scholar] [CrossRef] [PubMed]
- Walter, D.M.; McIntire, J.J.; Berry, G.; McKenzie, A.N.; Donaldson, D.D.; DeKruyff, R.H.; Umetsu, D.T. Critical role for IL-13 in the development of allergen-induced airway hyperreactivity. J. Immunol. 2001, 167, 4668–4675. [Google Scholar] [CrossRef] [PubMed]
- Cherry, W.B.; Yoon, J.; Bartemes, K.R.; Iijima, K.; Kita, H. A novel IL-1 family cytokine, IL-33, potently activates human eosinophils. J. Allergy Clin. Immunol. 2008, 121, 1484–1490. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Kondo, Y.; Yoshimoto, T.; Yasuda, K.; Futatsugi-Yumikura, S.; Morimoto, M.; Hayashi, N.; Hoshino, T.; Fujimoto, J.; Nakanishi, K. Administration of IL-33 induces airway hyperresponsiveness and goblet cell hyperplasia in the lungs in the absence of adaptive immune system. Int. Immunol. 2008, 20, 791–800. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Agusti, A.; Fabbri, L.M.; Singh, D.; Vestbo, J.; Celli, B.; Franssen, F.M.; Rabe, K.F.; Papi, A. Inhaled corticosteroids in COPD: Friend or foe? Eur. Respir. J. 2018. [Google Scholar] [CrossRef] [PubMed]
- Barnes, P.J. Targeting cytokines to treat asthma and chronic obstructive pulmonary disease. Nat. Rev. Immunol. 2018, 18, 454–466. [Google Scholar] [CrossRef] [PubMed]
- Rossi, A.; Khirani, S.; Cazzola, M. Long-acting β(2)-agonists (LABA) in chronic obstructive pulmonary disease: Efficacy and safety. Int. J. Chronic Obstr. Pulm. Dis. 2008, 3, 521–529. [Google Scholar] [CrossRef]
- Alagha, K.; Palot, A.; Sofalvi, T.; Pahus, L.; Gouitaa, M.; Tummino, C.; Martinez, S.; Charpin, D.; Bourdin, A.; Chanez, P. Long-acting muscarinic receptor antagonists for the treatment of chronic airway diseases. Ther. Adv. Chronic Dis. 2014, 5, 85–98. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Garth, J.M.; Mackel, J.J.; Reeder, K.M.; Blackburn, J.P.; Dunaway, C.W.; Yu, Z.; Matalon, S.; Fitz, L.; Steele, C. Acidic mammalian chitinase negatively affects immune responses during acute and chronic Aspergillus fumigatus exposure. Infect. Immunity 2018, 86, e00944-17. [Google Scholar] [CrossRef] [PubMed]
- Ross, K.R.; Chmiel, J.F.; Konstan, M.W. The Role of Inhaled Corticosteroids in the Management of Cystic Fibrosis. Paediatr. Drugs 2009, 11, 101–113. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Albert, R.K.; Connett, J.; Bailey, W.C.; Casaburi, R.; Cooper, J.A., Jr.; Criner, G.J.; Curtis, J.L.; Dransfield, M.T.; Han, M.K.; Lazarus, S.C.; et al. Azithromycin for prevention of exacerbations of COPD. N. Engl. J. Med. 2011, 365, 689–698. [Google Scholar] [CrossRef] [PubMed]
- Southern, K.W.; Barker, P.M.; Solis-Moya, A.; Patel, L. Macrolide antibiotics for cystic fibrosis. Cochrane Database Syst. Rev. 2012, 11, CD002203. [Google Scholar] [PubMed]
- Chong, J.; Leung, B.; Poole, P. Phosphodiesterase 4 inhibitors for chronic obstructive pulmonary disease. Cochrane Database Syst. Rev. 2017, 9, CD002309. [Google Scholar] [CrossRef] [PubMed]
- Barnes, P.J. Theophylline. Am. J. Respir. Crit. Care Med. 2013, 188, 901–906. [Google Scholar] [CrossRef] [PubMed]
- Shukla, D.; Chakraborty, S.; Singh, S.; Mishra, B. Doxofylline: A promising methylxanthine derivative for the treatment of asthma and chronic obstructive pulmonary disease. Expert Opin. Pharmacother. 2009, 10, 2343–2356. [Google Scholar] [CrossRef] [PubMed]
- Livraghi, A.; Randell, S.H. Cystic fibrosis and other respiratory diseases of impaired mucus clearance. Toxicol. Pathol. 2007, 35, 116–129. [Google Scholar] [CrossRef] [PubMed]
- Sloane, P.A.; Shastry, S.; Wilhelm, A.; Courville, C.; Tang, L.P.; Backer, K.; Levin, E.; Raju, S.V.; Li, Y.; Mazur, M.; et al. A pharmacologic approach to acquired cystic fibrosis transmembrane conductance regulator dysfunction in smoking related lung disease. PLoS ONE 2012, 7, e39809. [Google Scholar] [CrossRef] [PubMed]
- Flume, P.A.; Mogayzel, P.J., Jr.; Robinson, K.A.; Goss, C.H.; Rosenblatt, R.L.; Kuhn, R.J.; Marshall, B.C.; Clinical Practice Guidelines for Pulmonary Therapies Committee. Cystic fibrosis pulmonary guidelines: Treatment of pulmonary exacerbations. Am. J. Respir. Crit. Care Med. 2009, 180, 802–808. [Google Scholar] [CrossRef] [PubMed]
- Konstan, M.W.; Ratjen, F. Effect of dornase alfa on inflammation and lung function: Potential role in the early treatment of cystic fibrosis. J. Cyst. Fibros. 2012, 11, 78–83. [Google Scholar] [CrossRef] [PubMed]
- Rubin, B.K. Aerosol Medications for Treatment of Mucus Clearance Disorders. Respir. Care 2015, 60, 825–829; discussion 830–832. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Leckie, M.J.; ten Brinke, A.; Khan, J.; Diamant, Z.; O’Connor, B.J.; Walls, C.M.; Mathur, A.K.; Cowley, H.C.; Chung, K.F.; Djukanovic, R.; et al. Effects of an interleukin-5 blocking monoclonal antibody on eosinophils, airway hyper-responsiveness, and the late asthmatic response. Lancet 2000, 356, 2144–2148. [Google Scholar] [CrossRef]
- Pavord, I.D.; Korn, S.; Howarth, P.; Bleecker, E.R.; Buhl, R.; Keene, O.N.; Ortega, H.; Chanez, P. Mepolizumab for severe eosinophilic asthma (DREAM): A multicentre, double-blind, placebo-controlled trial. Lancet 2012, 380, 651–659. [Google Scholar] [CrossRef]
- Castro, M.; Wenzel, S.E.; Bleecker, E.R.; Pizzichini, E.; Kuna, P.; Busse, W.W.; Gossage, D.L.; Ward, C.K.; Wu, Y.; Wang, B.; et al. Benralizumab, an anti-interleukin 5 receptor alpha monoclonal antibody, versus placebo for uncontrolled eosinophilic asthma: A phase 2b randomised dose-ranging study. Lancet Respir. Med. 2014, 2, 879–890. [Google Scholar] [CrossRef]
- Deeks, E.D.; Brusselle, G. Reslizumab in Eosinophilic Asthma: A Review. Drugs 2017, 77, 777–784. [Google Scholar] [CrossRef] [PubMed]
- Brightling, C.E.; Bleecker, E.R.; Panettieri, R.A., Jr.; Bafadhel, M.; She, D.; Ward, C.K.; Xu, X.; Birrell, C.; van der Merwe, R. Benralizumab for chronic obstructive pulmonary disease and sputum eosinophilia: A randomised, double-blind, placebo-controlled, phase 2a study. Lancet Respir. Med. 2014, 2, 891–901. [Google Scholar] [CrossRef]
- Pavord, I.D.; Chanez, P.; Criner, G.J.; Kerstjens, H.A.M.; Korn, S.; Lugogo, N.; Martinot, J.-B.; Sagara, H.; Albers, F.C.; Bradford, E.S.; et al. Mepolizumab for Eosinophilic Chronic Obstructive Pulmonary Disease. N. Engl. J. Med. 2017, 377, 1613–1629. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Wenzel, S.; Castro, M.; Corren, J.; Maspero, J.; Wang, L.; Zhang, B.; Pirozzi, G.; Sutherland, E.R.; Evans, R.R.; Joish, V.N.; et al. Dupilumab efficacy and safety in adults with uncontrolled persistent asthma despite use of medium-to-high-dose inhaled corticosteroids plus a long-acting beta2 agonist: A randomised double-blind placebo-controlled pivotal phase 2b dose-ranging trial. Lancet 2016, 388, 31–44. [Google Scholar] [CrossRef]
- Wenzel, S.; Ford, L.; Pearlman, D.; Spector, S.; Sher, L.; Skobieranda, F.; Wang, L.; Kirkesseli, S.; Rocklin, R.; Bock, B.; et al. Dupilumab in persistent asthma with elevated eosinophil levels. N. Engl. J. Med. 2013, 368, 2455–2466. [Google Scholar] [CrossRef] [PubMed]
- Wechsler, M.E. Current and Emerging Biologic Therapies for Asthma and COPD. Respir. Care 2018, 63, 699–707. [Google Scholar] [CrossRef] [PubMed]
- Lappalainen, U.; Whitsett, J.A.; Wert, S.E.; Tichelaar, J.W.; Bry, K. Interleukin-1beta causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung. Am. J. Respir. Cell Mol. Biol. 2005, 32, 311–318. [Google Scholar] [CrossRef] [PubMed]
- Dinarello, C.A. Immunological and inflammatory functions of the interleukin-1 family. Annu. Rev. Immunol. 2009, 27, 519–550. [Google Scholar] [CrossRef] [PubMed]
- Lamkanfi, M. Emerging inflammasome effector mechanisms. Nat. Rev. Immunol. 2011, 11, 213–220. [Google Scholar] [CrossRef] [PubMed]
- Miao, E.A.; Ernst, R.K.; Dors, M.; Mao, D.P.; Aderem, A. Pseudomonas aeruginosa activates caspase 1 through Ipaf. Proc. Natl. Acad. Sci. USA 2008, 105, 2562–2567. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Douglas, T.A.; Brennan, S.; Gard, S.; Berry, L.; Gangell, C.; Stick, S.M.; Clements, B.S.; Sly, P.D. Acquisition and eradication of P. aeruginosa in young children with cystic fibrosis. Eur. Respir. J. 2009, 33, 305–311. [Google Scholar] [CrossRef] [PubMed]
- Levy, H.; Murphy, A.; Zou, F.; Gerard, C.; Klanderman, B.; Schuemann, B.; Lazarus, R.; Garcia, K.C.; Celedon, J.C.; Drumm, M.; et al. IL1B polymorphisms modulate cystic fibrosis lung disease. Pediatr. Pulmonol. 2009, 44, 580–593. [Google Scholar] [CrossRef] [PubMed]
- Muselet-Charlier, C.; Roque, T.; Boncoeur, E.; Chadelat, K.; Clement, A.; Jacquot, J.; Tabary, O. Enhanced IL-1beta-induced IL-8 production in cystic fibrosis lung epithelial cells is dependent of both mitogen-activated protein kinases and NF-kappaB signaling. Biochem. Biophys. Res. Commun. 2007, 357, 402–407. [Google Scholar] [CrossRef] [PubMed]
- Palomo, J.; Marchiol, T.; Piotet, J.; Fauconnier, L.; Robinet, M.; Reverchon, F.; Le Bert, M.; Togbe, D.; Buijs-Offerman, R.; Stolarczyk, M.; et al. Role of IL-1beta in experimental cystic fibrosis upon P. aeruginosa infection. PLoS ONE 2014, 9, e114884. [Google Scholar] [CrossRef] [PubMed]
- Iannitti, R.G.; Napolioni, V.; Oikonomou, V.; De Luca, A.; Galosi, C.; Pariano, M.; Massi-Benedetti, C.; Borghi, M.; Puccetti, M.; Lucidi, V.; et al. IL-1 receptor antagonist ameliorates inflammasome-dependent inflammation in murine and human cystic fibrosis. Nat. Commun. 2016, 7, 10791. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Gao, P.; Gibson, P.G.; Baines, K.J.; Yang, I.A.; Upham, J.W.; Reynolds, P.N.; Hodge, S.; James, A.L.; Jenkins, C.; Peters, M.J.; et al. Anti-inflammatory deficiencies in neutrophilic asthma: Reduced galectin-3 and IL-1RA/IL-1beta. Respir. Res. 2015, 16, 5. [Google Scholar] [CrossRef] [PubMed]
- He, Y.; Peng, S.; Xiong, W.; Xu, Y.; Liu, J. Association between polymorphism of interleukin-1 beta and interleukin-1 receptor antagonist gene and asthma risk: A meta-analysis. ScientificWorldJournal 2015, 2015, 685684. [Google Scholar] [CrossRef] [PubMed]
- Besnard, A.G.; Togbe, D.; Couillin, I.; Tan, Z.; Zheng, S.G.; Erard, F.; Le Bert, M.; Quesniaux, V.; Ryffel, B. Inflammasome-IL-1-Th17 response in allergic lung inflammation. J. Mol. Cell Biol. 2012, 4, 3–10. [Google Scholar] [CrossRef] [PubMed]
- Whelan, R.; Kim, C.; Chen, M.; Leiter, J.; Grunstein, M.M.; Hakonarson, H. Role and regulation of interleukin-1 molecules in pro-asthmatic sensitised airway smooth muscle. Eur. Respir. J. 2004, 24, 559–567. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Rogliani, P.; Calzetta, L.; Ora, J.; Matera, M.G. Canakinumab for the treatment of chronic obstructive pulmonary disease. Pulm. Pharmacol. Ther. 2015, 31, 15–27. [Google Scholar] [CrossRef] [PubMed]
- Hernandez, M.L.; Mills, K.; Almond, M.; Todoric, K.; Aleman, M.M.; Zhang, H.; Zhou, H.; Peden, D.B. IL-1 receptor antagonist reduces endotoxin-induced airway inflammation in healthy volunteers. J. Allergy Clin. Immunol. 2015, 135, 379–385. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Montgomery, S.T.; Mall, M.A.; Kicic, A.; Stick, S.M.; Arest, C.F. Hypoxia and sterile inflammation in cystic fibrosis airways: Mechanisms and potential therapies. Eur. Respir. J. 2017, 49, 1600903. [Google Scholar] [CrossRef] [PubMed]
- Marini, M.; Vittori, E.; Hollemborg, J.; Mattoli, S. Expression of the potent inflammatory cytokines, granulocyte-macrophage-colony-stimulating factor and interleukin-6 and interleukin-8, in bronchial epithelial cells of patients with asthma. J. Allergy Clin. Immunol. 1992, 89, 1001–1009. [Google Scholar] [CrossRef]
- Stadnyk, A.W. Cytokine production by epithelial cells. FASEB J. 1994, 8, 1041–1047. [Google Scholar] [CrossRef] [PubMed]
- Yokoyama, A.; Kohno, N.; Fujino, S.; Hamada, H.; Inoue, Y.; Fujioka, S.; Ishida, S.; Hiwada, K. Circulating interleukin-6 levels in patients with bronchial asthma. Am. J. Respir. Crit. Care Med. 1995, 151, 1354–1358. [Google Scholar] [CrossRef] [PubMed]
- Franceschi, C.; Capri, M.; Monti, D.; Giunta, S.; Olivieri, F.; Sevini, F.; Panourgia, M.P.; Invidia, L.; Celani, L.; Scurti, M.; et al. Inflammaging and anti-inflammaging: A systemic perspective on aging and longevity emerged from studies in humans. Mech. Ageing Dev. 2007, 128, 92–105. [Google Scholar] [CrossRef] [PubMed]
- Barnes, P.J. Future treatments for chronic obstructive pulmonary disease and its comorbidities. Proc. Am. Thorac. Soc. 2008, 5, 857–864. [Google Scholar] [CrossRef] [PubMed]
- Agrawal, A.; Tay, J.; Ton, S.; Agrawal, S.; Gupta, S. Increased reactivity of dendritic cells from aged subjects to self-antigen, the human DNA. J. Immunol. 2009, 182, 1138–1145. [Google Scholar] [CrossRef] [PubMed]
- Sharma, G.; Hanania, N.A.; Shim, Y.M. The aging immune system and its relationship to the development of chronic obstructive pulmonary disease. Proc. Am. Thorac. Soc. 2009, 6, 573–580. [Google Scholar] [CrossRef] [PubMed]
- Mehrotra, N.; Freire, A.X.; Bauer, D.C.; Harris, T.B.; Newman, A.B.; Kritchevsky, S.B.; Meibohm, B.; Health, A.B.C.S. Predictors of mortality in elderly subjects with obstructive airway disease: The PILE score. Ann. Epidemiol. 2010, 20, 223–232. [Google Scholar] [CrossRef] [PubMed]
- Nixon, L.S.; Yung, B.; Bell, S.C.; Elborn, J.S.; Shale, D.J. Circulating immunoreactive interleukin-6 in cystic fibrosis. Am. J. Respir. Crit. Care Med. 1998, 157, 1764–1769. [Google Scholar] [CrossRef] [PubMed]
- Neveu, W.A.; Bernardo, E.; Allard, J.L.; Nagaleekar, V.; Wargo, M.J.; Davis, R.J.; Iwakura, Y.; Whittaker, L.A.; Rincon, M. Fungal allergen beta-glucans trigger p38 mitogen-activated protein kinase-mediated IL-6 translation in lung epithelial cells. Am. J. Respir. Cell Mol. Biol. 2011, 45, 1133–1141. [Google Scholar] [CrossRef] [PubMed]
- Dienz, O.; Rincon, M. The effects of IL-6 on CD4 T cell responses. Clin. Immunol. 2009, 130, 27–33. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Dienz, O.; Eaton, S.M.; Bond, J.P.; Neveu, W.; Moquin, D.; Noubade, R.; Briso, E.M.; Charland, C.; Leonard, W.J.; Ciliberto, G.; et al. The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cells. J. Exp. Med. 2009, 206, 69–78. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Tillie-Leblond, I.; Pugin, J.; Marquette, C.H.; Lamblin, C.; Saulnier, F.; Brichet, A.; Wallaert, B.; Tonnel, A.B.; Gosset, P. Balance between proinflammatory cytokines and their inhibitors in bronchial lavage from patients with status asthmaticus. Am. J. Respir. Crit. Care Med. 1999, 159, 487–494. [Google Scholar] [CrossRef] [PubMed]
- Virchow, J.C., Jr.; Kroegel, C.; Walker, C.; Matthys, H. Inflammatory determinants of asthma severity: Mediator and cellular changes in bronchoalveolar lavage fluid of patients with severe asthma. J. Allergy Clin. Immunol. 1996, 98, S27–S33; discussion S33–S40. [Google Scholar] [CrossRef]
- Dixon, A.E.; Raymond, D.M.; Suratt, B.T.; Bourassa, L.M.; Irvin, C.G. Lower airway disease in asthmatics with and without rhinitis. Lung 2008, 186, 361–368. [Google Scholar] [CrossRef] [PubMed]
- Morjaria, J.B.; Babu, K.S.; Vijayanand, P.; Chauhan, A.J.; Davies, D.E.; Holgate, S.T. Sputum IL-6 concentrations in severe asthma and its relationship with FEV1. Thorax 2011, 66, 537. [Google Scholar] [CrossRef] [PubMed]
- Dixon, A.E.; Shade, D.M.; Cohen, R.I.; Skloot, G.S.; Holbrook, J.T.; Smith, L.J.; Lima, J.J.; Allayee, H.; Irvin, C.G.; Wise, R.A.; et al. Effect of obesity on clinical presentation and response to treatment in asthma. J. Asthma 2006, 43, 553–558. [Google Scholar] [CrossRef] [PubMed]
- Kishimoto, T. IL-6: From its discovery to clinical applications. Int. Immunol. 2010, 22, 347–352. [Google Scholar] [CrossRef] [PubMed]
- Kelly, E.; Greene, C.M.; McElvaney, N.G. Targeting neutrophil elastase in cystic fibrosis. Expert Opin. Ther. Targets 2008, 12, 145–157. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Nakamura, H.; Yoshimura, K.; McElvaney, N.G.; Crystal, R.G. Neutrophil elastase in respiratory epithelial lining fluid of individuals with cystic fibrosis induces interleukin-8 gene expression in a human bronchial epithelial cell line. J. Clin. Investig. 1992, 89, 1478–1484. [Google Scholar] [CrossRef] [PubMed]
- Lampronti, I.; Manzione, M.G.; Sacchetti, G.; Ferrari, D.; Spisani, S.; Bezzerri, V.; Finotti, A.; Borgatti, M.; Dechecchi, M.C.; Miolo, G.; et al. Differential Effects of Angelicin Analogues on NF-kappaB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells. Mediat. Inflamm. 2017, 2017, 2389487. [Google Scholar] [CrossRef] [PubMed]
- Jaudszus, A.; Arnold, C.; Hentschel, J.; Hunniger, K.; Baier, M.; Mainz, J.G. Increased cytokines in cystic fibrosis patients’ upper airways during a new P. aeruginosa colonization. Pediatr. Pulmonol. 2018, 53, 881–887. [Google Scholar] [CrossRef] [PubMed]
- Weber, A.; Wasiliew, P.; Kracht, M. Interleukin-1beta (IL-1beta) processing pathway. Sci. Signal. 2010, 3, cm2. [Google Scholar] [PubMed]
- Scheid, P.; Kempster, L.; Griesenbach, U.; Davies, J.C.; Dewar, A.; Weber, P.P.; Colledge, W.H.; Evans, M.J.; Geddes, D.M.; Alton, E.W. Inflammation in cystic fibrosis airways: Relationship to increased bacterial adherence. Eur. Respir. J. 2001, 17, 27–35. [Google Scholar] [CrossRef] [PubMed]
- Larsson, K. Inflammatory markers in COPD. Clin. Respir. J. 2008, 2 (Suppl. 1), 84–87. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Bautista, M.V.; Chen, Y.; Ivanova, V.S.; Rahimi, M.K.; Watson, A.M.; Rose, M.C. IL-8 regulates mucin gene expression at the posttranscriptional level in lung epithelial cells. J. Immunol. 2009, 183, 2159–2166. [Google Scholar] [CrossRef] [PubMed]
- Schneider, D.; Ganesan, S.; Comstock, A.T.; Meldrum, C.A.; Mahidhara, R.; Goldsmith, A.M.; Curtis, J.L.; Martinez, F.J.; Hershenson, M.B.; Sajjan, U. Increased cytokine response of rhinovirus-infected airway epithelial cells in chronic obstructive pulmonary disease. Am. J. Respir. Crit. Care Med. 2010, 182, 332–340. [Google Scholar] [CrossRef] [PubMed]
- Sarir, H.; Mortaz, E.; Janse, W.T.; Givi, M.E.; Nijkamp, F.P.; Folkerts, G. IL-8 production by macrophages is synergistically enhanced when cigarette smoke is combined with TNF-alpha. Biochem. Pharmacol. 2010, 79, 698–705. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Zheng, H.; Zhang, H.; Ma, W.; Wang, F.; Liu, C.; He, S. Increased interleukin (IL)-8 and decreased IL-17 production in chronic obstructive pulmonary disease (COPD) provoked by cigarette smoke. Cytokine 2011, 56, 717–725. [Google Scholar] [CrossRef] [PubMed]
- Hiemstra, P.S. Altered macrophage function in chronic obstructive pulmonary disease. Ann. Am. Thorac. Soc. 2013, 10, S180–S185. [Google Scholar] [CrossRef] [PubMed]
- Dibbert, B.; Weber, M.; Nikolaizik, W.H.; Vogt, P.; Schoni, M.H.; Blaser, K.; Simon, H.U. Cytokine-mediated Bax deficiency and consequent delayed neutrophil apoptosis: A general mechanism to accumulate effector cells in inflammation. Proc. Natl. Acad. Sci. USA 1999, 96, 13330–13335. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Huang, A.X.; Lu, L.W.; Liu, W.J.; Huang, M. Plasma Inflammatory Cytokine IL-4, IL-8, IL-10, and TNF-alpha Levels Correlate with Pulmonary Function in Patients with Asthma-Chronic Obstructive Pulmonary Disease (COPD) Overlap Syndrome. Med. Sci. Monit. 2016, 22, 2800–2808. [Google Scholar] [CrossRef] [PubMed]
- Jatakanon, A.; Uasuf, C.; Maziak, W.; Lim, S.; Chung, K.F.; Barnes, P.J. Neutrophilic inflammation in severe persistent asthma. Am. J. Respir. Crit. Care Med. 1999, 160 Pt 1, 1532–1539. [Google Scholar] [CrossRef]
- Ordonez, C.L.; Shaughnessy, T.E.; Matthay, M.A.; Fahy, J.V. Increased neutrophil numbers and IL-8 levels in airway secretions in acute severe asthma: Clinical and biologic significance. Am. J. Respir. Crit. Care Med. 2000, 161 Pt 1, 1185–1190. [Google Scholar] [CrossRef]
- Lamblin, C.; Gosset, P.; Tillie-Leblond, I.; Saulnier, F.; Marquette, C.H.; Wallaert, B.; Tonnel, A.B. Bronchial neutrophilia in patients with noninfectious status asthmaticus. Am. J. Respir. Crit. Care Med. 1998, 157, 394–402. [Google Scholar] [CrossRef] [PubMed]
- Hosoki, K.; Ying, S.; Corrigan, C.; Qi, H.; Kurosky, A.; Jennings, K.; Sun, Q.; Boldogh, I.; Sur, S. Analysis of a Panel of 48 Cytokines in BAL Fluids Specifically Identifies IL-8 Levels as the Only Cytokine that Distinguishes Controlled Asthma from Uncontrolled Asthma, and Correlates Inversely with FEV1. PLoS ONE 2015, 10, e0126035. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Mahler, D.A.; Huang, S.; Tabrizi, M.; Bell, G.M. Efficacy and safety of a monoclonal antibody recognizing interleukin-8 in COPD: A pilot study. Chest 2004, 126, 926–934. [Google Scholar] [CrossRef] [PubMed]
- Lazaar, A.L.; Miller, B.E.; Tabberer, M.; Yonchuk, J.; Leidy, N.; Ambery, C.; Bloomer, J.; Watz, H.; Tal-Singer, R. Effect of the CXCR2 antagonist danirixin on symptoms and health status in COPD. Eur. Respir. J. 2018, 52, 1801020. [Google Scholar] [CrossRef] [PubMed]
- Rennard, S.I.; Dale, D.C.; Donohue, J.F.; Kanniess, F.; Magnussen, H.; Sutherland, E.R.; Watz, H.; Lu, S.; Stryszak, P.; Rosenberg, E.; et al. CXCR2 Antagonist MK-7123. A Phase 2 Proof-of-Concept Trial for Chronic Obstructive Pulmonary Disease. Am. J. Respir. Crit. Care Med. 2015, 191, 1001–1011. [Google Scholar] [CrossRef] [PubMed]
- Moss, R.B.; Mistry, S.J.; Konstan, M.W.; Pilewski, J.M.; Kerem, E.; Tal-Singer, R.; Lazaar, A.L. Safety and early treatment effects of the CXCR2 antagonist SB-656933 in patients with cystic fibrosis. J. Cyst. Fibros. 2013, 12, 241–248. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Linden, A.; Laan, M.; Anderson, G.P. Neutrophils, interleukin-17A and lung disease. Eur. Respir. J. 2005, 25, 159–172. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Nembrini, C.; Marsland, B.J.; Kopf, M. IL-17-producing T cells in lung immunity and inflammation. J. Allergy Clin. Immunol. 2009, 123, 986–994. [Google Scholar] [CrossRef] [PubMed]
- Di Stefano, A.; Caramori, G.; Gnemmi, I.; Contoli, M.; Vicari, C.; Capelli, A.; Magno, F.; D’Anna, S.E.; Zanini, A.; Brun, P.; et al. T helper type 17-related cytokine expression is increased in the bronchial mucosa of stable chronic obstructive pulmonary disease patients. Clin. Exp. Immunol. 2009, 157, 316–324. [Google Scholar] [CrossRef] [PubMed]
- Li, L.; Huang, L.; Vergis, A.L.; Ye, H.; Bajwa, A.; Narayan, V.; Strieter, R.M.; Rosin, D.L.; Okusa, M.D. IL-17 produced by neutrophils regulates IFN-gamma-mediated neutrophil migration in mouse kidney ischemia-reperfusion injury. J. Clin. Investig. 2010, 120, 331–342. [Google Scholar] [CrossRef] [PubMed]
- Eustace, A.; Smyth, L.J.C.; Mitchell, L.; Williamson, K.; Plumb, J.; Singh, D. Identification of cells expressing IL-17A and IL-17F in the lungs of patients with COPD. Chest 2011, 139, 1089–1100. [Google Scholar] [CrossRef] [PubMed]
- Doe, C.; Bafadhel, M.; Siddiqui, S.; Desai, D.; Mistry, V.; Rugman, P.; McCormick, M.; Woods, J.; May, R.; Sleeman, M.A.; et al. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD. Chest 2010, 138, 1140–1147. [Google Scholar] [CrossRef] [PubMed]
- Pridgeon, C.; Bugeon, L.; Donnelly, L.; Straschil, U.; Tudhope, S.J.; Fenwick, P.; Lamb, J.R.; Barnes, P.J.; Dallman, M.J. Regulation of IL-17 in chronic inflammation in the human lung. Clin. Sci. 2011, 120, 515–524. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Wiehler, S.; Proud, D. Interleukin-17A modulates human airway epithelial responses to human rhinovirus infection. Am. J. Physiol. Lung Cell Mol. Physiol. 2007, 293, L505-15. [Google Scholar] [CrossRef] [PubMed]
- Fujisawa, T.; Velichko, S.; Thai, P.; Hung, L.Y.; Huang, F.; Wu, R. Regulation of airway MUC5AC expression by IL-1beta and IL-17A; the NF-kappaB paradigm. J. Immunol. 2009, 183, 6236–6243. [Google Scholar] [CrossRef] [PubMed]
- Brodlie, M.; McKean, M.C.; Johnson, G.E.; Anderson, A.E.; Hilkens, C.M.; Fisher, A.J.; Corris, P.A.; Lordan, J.L.; Ward, C. Raised interleukin-17 is immunolocalised to neutrophils in cystic fibrosis lung disease. Eur. Respir. J. 2011, 37, 1378–1385. [Google Scholar] [CrossRef] [PubMed]
- Hsu, D.; Taylor, P.; Fletcher, D.; van Heeckeren, R.; Eastman, J.; van Heeckeren, A.; Davis, P.; Chmiel, J.F.; Pearlman, E.; Bonfield, T.L. Interleukin-17 Pathophysiology and Therapeutic Intervention in Cystic Fibrosis Lung Infection and Inflammation. Infect. Immun. 2016, 84, 2410–2421. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Alcorn, J.F.; Crowe, C.R.; Kolls, J.K. TH17 cells in asthma and COPD. Annu. Rev. Physiol. 2010, 72, 495–516. [Google Scholar] [CrossRef] [PubMed]
- Chan, Y.R.; Chen, K.; Duncan, S.R.; Lathrop, K.L.; Latoche, J.D.; Logar, A.J.; Pociask, D.A.; Wahlberg, B.J.; Ray, P.; Ray, A.; et al. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. J. Allergy Clin. Immunol. 2013, 131, 1117–1129.e5. [Google Scholar] [CrossRef] [PubMed][Green Version]
- McAleer, J.P.; Kolls, J.K. Directing traffic: IL-17 and IL-22 coordinate pulmonary immune defense. Immunol. Rev. 2014, 260, 129–144. [Google Scholar] [CrossRef] [PubMed][Green Version]
- Busse, W.W.; Holgate, S.; Kerwin, E.; Chon, Y.; Feng, J.; Lin, J.; Lin, S.L. Randomized, double-blind, placebo-controlled study of brodalumab, a human anti-IL-17 receptor monoclonal antibody, in moderate to severe asthma. Am. J. Respir. Crit. Care Med. 2013, 188, 1294–1302. [Google Scholar] [CrossRef] [PubMed]
- Eich, A.; Urban, V.; Jutel, M.; Vlcek, J.; Shim, J.J.; Trofimov, V.I.; Liam, C.K.; Kuo, P.H.; Hou, Y.; Xiao, J.; et al. A Randomized, Placebo-Controlled Phase 2 Trial of CNTO 6785 in Chronic Obstructive Pulmonary Disease. COPD 2017, 14, 476–483. [Google Scholar] [CrossRef] [PubMed]
Figure 1. Diagram depicting inflammatory airway diseases in circles (allergic asthma, COPD and bronchiectasis), which all show airway inflammation with involvement of various cell types and the main cytokines (red rectangles), and current common therapies (green/blue rectangles) used for their treatment. Abbreviations: SABA, short-acting β2-adrenoreceptor agonists; LABA, long-acting β2-adrenoreceptor agonists; MABA, dual muscarinic antagonist-β2-agonist compounds; PDE4, phosphodiesterase 4; SAMA, short-acting muscarinic receptor antagonists, LAMA, long-acting muscarinic receptor antagonists; ICS, inhaled corticosteroids; NSAIDs, nonsteroidal anti-inflammatory drugs.
Figure 2. Diagram depicting current and future cytokine specific therapy options, with pink boxes representing established therapies and light green boxes representing therapies that are currently under investigation—cytokines are in either red or green.
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).