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In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota

1
DSM Nutritional Products Ltd., R&D Human Nutrition and Health, 4002 Basel, Switzerland
2
The Netherlands Organization for Applied Scientific Research (TNO), Microbiology and Systems Biology, 3704 HE Zeist, The Netherlands
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(10), 3097; https://doi.org/10.3390/ijms19103097
Received: 31 August 2018 / Revised: 27 September 2018 / Accepted: 3 October 2018 / Published: 10 October 2018
(This article belongs to the Special Issue Dietary Fibre: New Insights on Biochemistry and Health Benefits)
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Abstract

Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli. Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied. An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition). All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers. Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production. Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition. A dose-response effect was observed for butyrate when exposed to beta-glucan and inulin. The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits. View Full-Text
Keywords: XOS; beta-glucan; alpha-GOS; screening platform; i-screen; colonic fermentation XOS; beta-glucan; alpha-GOS; screening platform; i-screen; colonic fermentation
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Fehlbaum, S.; Prudence, K.; Kieboom, J.; Heerikhuisen, M.; Van den Broek, T.; Schuren, F.H.J.; Steinert, R.E.; Raederstorff, D. In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota. Int. J. Mol. Sci. 2018, 19, 3097.

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