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Article

In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota

1
DSM Nutritional Products Ltd., R&D Human Nutrition and Health, 4002 Basel, Switzerland
2
The Netherlands Organization for Applied Scientific Research (TNO), Microbiology and Systems Biology, 3704 HE Zeist, The Netherlands
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(10), 3097; https://doi.org/10.3390/ijms19103097
Received: 31 August 2018 / Revised: 27 September 2018 / Accepted: 3 October 2018 / Published: 10 October 2018
(This article belongs to the Special Issue Dietary Fibre: New Insights on Biochemistry and Health Benefits)
Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli. Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied. An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition). All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers. Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production. Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition. A dose-response effect was observed for butyrate when exposed to beta-glucan and inulin. The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits. View Full-Text
Keywords: XOS; beta-glucan; alpha-GOS; screening platform; i-screen; colonic fermentation XOS; beta-glucan; alpha-GOS; screening platform; i-screen; colonic fermentation
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MDPI and ACS Style

Fehlbaum, S.; Prudence, K.; Kieboom, J.; Heerikhuisen, M.; Van den Broek, T.; Schuren, F.H.J.; Steinert, R.E.; Raederstorff, D. In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota. Int. J. Mol. Sci. 2018, 19, 3097. https://doi.org/10.3390/ijms19103097

AMA Style

Fehlbaum S, Prudence K, Kieboom J, Heerikhuisen M, Van den Broek T, Schuren FHJ, Steinert RE, Raederstorff D. In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota. International Journal of Molecular Sciences. 2018; 19(10):3097. https://doi.org/10.3390/ijms19103097

Chicago/Turabian Style

Fehlbaum, Sophie, Kevin Prudence, Jasper Kieboom, Margreet Heerikhuisen, Tim Van den Broek, Frank H. J. Schuren, Robert E. Steinert, and Daniel Raederstorff. 2018. "In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota" International Journal of Molecular Sciences 19, no. 10: 3097. https://doi.org/10.3390/ijms19103097

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