Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. C8-ceramide Exerts the Anti-Proliferation Potential against H1299 Lung Cancer Cells.
2.2. C8-Ceramide May Cause the G1 Arrest of H1299 Cells
2.3. The Detection of Endogenous ROS in C8-Ceramide-Treated H1299 Cells
2.4. Assessment of Migration in C8-ceramide-treated H1299 cells
2.5. The Modulation of SOD1 and SOD2 in C8-Ceramide Treated H1299 Cells
3. Discussion
4. Materials and Methods
4.1. Preparation of C8-Ceramide
4.2. Cell Cultures
4.3. Cell Proliferation Assay
4.4. Apoptosis Assessment
4.5. Cell Cycle Distribution
4.6. Flow Cytometry-based ROS assessment
4.7. Wound Healing Assay
4.8. Boyden’s Transwell Assay
4.9. Western Blotting Assay
4.10. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chang, Y.C.; Fong, Y.; Tsai, E.-M.; Chang, Y.-G.; Chou, H.L.; Wu, C.-Y.; Teng, Y.-N.; Liu, T.-C.; Yuan, S.-S.; Chiu, C.-C. Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells. Int. J. Mol. Sci. 2018, 19, 3010. https://doi.org/10.3390/ijms19103010
Chang YC, Fong Y, Tsai E-M, Chang Y-G, Chou HL, Wu C-Y, Teng Y-N, Liu T-C, Yuan S-S, Chiu C-C. Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells. International Journal of Molecular Sciences. 2018; 19(10):3010. https://doi.org/10.3390/ijms19103010
Chicago/Turabian StyleChang, Yuli C., Yao Fong, Eing-Mei Tsai, Ya-Gin Chang, Han Lin Chou, Chang-Yi Wu, Yen-Ni Teng, Ta-Chih Liu, Shyng-Shiou Yuan, and Chien-Chih Chiu. 2018. "Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells" International Journal of Molecular Sciences 19, no. 10: 3010. https://doi.org/10.3390/ijms19103010