Next Article in Journal
Functional Testing of SLC26A4 Variants—Clinical and Molecular Analysis of a Cohort with Enlarged Vestibular Aqueduct from Austria
Next Article in Special Issue
Enhancement in Corneal Permeability of Dissolved Carteolol by Its Combination with Magnesium Hydroxide Nanoparticles
Previous Article in Journal
In Silico Analysis of the Association Relationship between Neuroprotection and Flavors of Traditional Chinese Medicine Based on the mGluRs
Previous Article in Special Issue
Spatiotemporal Control of Doxorubicin Delivery from “Stealth-Like” Prodrug Micelles
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2018, 19(1), 205;

Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration

Key Centre for Polymers and Colloids, School of Chemistry, University of Sydney, Sydney, NSW 2006, Australia
Bill Walsh Translational Cancer Research Laboratory, Kolling Institute, Royal North Shore Hospital, Sydney, NSW 2065, Australia
Sydney Medical School—Northern, University of Sydney, Sydney, NSW 2006, Australia
Sydney School of Veterinary Science, University of Sydney Teaching Hospital Camden, Camden, NSW 2570, Australia
Sirtex Medical Limited, North Sydney, NSW 2060, Australia
Binh T. T. Pham and Emily K. Colvin contributed equally, Viive M. Howell and Brian S. Hawkett contributed equally.
Current address: Barry H. Rickman, Sound VetPath, Edmonds, WA 98020, USA; Nicole S. Bryce, School of Medical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Authors to whom correspondence should be addressed.
Received: 12 September 2017 / Revised: 17 December 2017 / Accepted: 27 December 2017 / Published: 10 January 2018
(This article belongs to the Special Issue Nanotechnology in Drug Delivery)
Full-Text   |   PDF [6543 KB, uploaded 10 January 2018]   |  


Nanomedicine is an emerging field with great potential in disease theranostics. We generated sterically stabilized superparamagnetic iron oxide nanoparticles (s-SPIONs) with average core diameters of 10 and 25 nm and determined the in vivo biodistribution and clearance profiles. Healthy nude mice underwent an intraperitoneal injection of these s-SPIONs at a dose of 90 mg Fe/kg body weight. Tissue iron biodistribution was monitored by atomic absorption spectroscopy and Prussian blue staining. Histopathological examination was performed to assess tissue toxicity. The 10 nm s-SPIONs resulted in higher tissue-iron levels, whereas the 25 nm s-SPIONs peaked earlier and cleared faster. Increased iron levels were detected in all organs and body fluids tested except for the brain, with notable increases in the liver, spleen, and the omentum. The tissue-iron returned to control or near control levels within 7 days post-injection, except in the omentum, which had the largest and most variable accumulation of s-SPIONs. No obvious tissue changes were noted although an influx of macrophages was observed in several tissues suggesting their involvement in s-SPION sequestration and clearance. These results demonstrate that the s-SPIONs do not degrade or aggregate in vivo and intraperitoneal administration is well tolerated, with a broad and transient biodistribution. In an ovarian tumor model, s-SPIONs were shown to accumulate in the tumors, highlighting their potential use as a chemotherapy delivery agent. View Full-Text

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Pham, B.T.T.; Colvin, E.K.; Pham, N.T.H.; Kim, B.J.; Fuller, E.S.; Moon, E.A.; Barbey, R.; Yuen, S.; Rickman, B.H.; Bryce, N.S.; Bickley, S.; Tanudji, M.; Jones, S.K.; Howell, V.M.; Hawkett, B.S. Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration. Int. J. Mol. Sci. 2018, 19, 205.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top