Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays
Abstract
:1. Introduction
2. Results
2.1. Nrf2 Knockout Improved the Survival of Mice after Abdominal Irradiation
2.2. Nrf2 Knockout Increased the Immune Organ Coefficient of Mice after Abdominal Irradiation
2.3. Nrf2 Knockout Mitigated Peripheral Blood Lymphocyte Count Decreases of Mice after Abdominal Irradiation
2.4. Nrf2 Knockout Attenuated DNA Damage to Peripheral Blood Lymphocytes of Mice After Abdominal Irradiation
2.5. Nrf2 Knockout Alleviated the Damage to Crypt-Villus of the Small Intestine from Mice after Abdominal Irradiation
2.6. Nrf2 Knockout Decreased the Rate of Apoptosis of the Small Intestine from Mice after Abdominal Irradiation
2.7. Nrf2 Knockout Resulted in an Increase in Lgr5+ Intestinal Stem Cells and Their Progeny of the Small Intestine from Mice after Abdominal Irradiation
2.8. Nrf2 Knockout Enhanced the Activation of NF-κB of the Small Intestine from Mice after Abdominal Irradiation
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Irradiation of Mice
4.3. Analysis of the 30-Day Survival Rate
4.4. Organ Coefficient Calculation
4.5. Peripheral Blood Cells Counts
4.6. Analysis of DNA Damage to Peripheral Blood Lymphocytes
4.7. Pathological Analysis
4.8. Immunohistochemical Analysis
4.9. Apoptosis Assay
4.10. Isolation of Crypt Single Cells
4.11. Preparation of Cytoplasmic and Nuclear Extracts
4.12. Western Blot Analysis
4.13. RNA Isolation and Real-Time Polymerase Chain Reaction
4.14. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yang, W.; Sun, Z.; Yang, B.; Wang, Q. Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays. Int. J. Mol. Sci. 2017, 18, 1656. https://doi.org/10.3390/ijms18081656
Yang W, Sun Z, Yang B, Wang Q. Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays. International Journal of Molecular Sciences. 2017; 18(8):1656. https://doi.org/10.3390/ijms18081656
Chicago/Turabian StyleYang, Wenyan, Zhijuan Sun, Bing Yang, and Qin Wang. 2017. "Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays" International Journal of Molecular Sciences 18, no. 8: 1656. https://doi.org/10.3390/ijms18081656