Next Article in Journal
Molecular, Genetic and Agronomic Approaches to Utilizing Pulses as Cover Crops and Green Manure into Cropping Systems
Next Article in Special Issue
Proteases of Dermatophagoides pteronyssinus
Previous Article in Journal
Genomic Variations in Pancreatic Cancer and Potential Opportunities for Development of New Approaches for Diagnosis and Treatment
Previous Article in Special Issue
Conformational Flexibility Differentiates Naturally Occurring Bet v 1 Isoforms
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(6), 1199;

Identification of Proteases and Protease Inhibitors in Allergenic and Non-Allergenic Pollen

Deptartment of Molecular Biology, University of Salzburg, A-5020 Salzburg, Austria
Faculty of Agricultural Sciences, Georg-August-Universität Göttingen, D-37075 Göttingen, Germany
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Joseph V. Moxon
Received: 28 April 2017 / Revised: 24 May 2017 / Accepted: 1 June 2017 / Published: 5 June 2017
(This article belongs to the Special Issue Proteolysis in Allergic Sensitization and Th2 Response)
Full-Text   |   PDF [829 KB, uploaded 5 June 2017]   |  


Pollen is one of the most common causes of allergy worldwide, making the study of their molecular composition crucial for the advancement of allergy research. Despite substantial efforts in this field, it is not yet clear why some plant pollens strongly provoke allergies while others do not. However, proteases and protease inhibitors from allergen sources are known to play an important role in the development of pollen allergies. In this study, we aim to uncover differences in the transcriptional pattern of proteases and protease inhibitors in Betula verrucosa and Pinus sylvestris pollen as models for high and low allergenic potential, respectively. We applied RNA sequencing to Betula verrucosa and Pinus sylvestris pollen. After de-novo assembly we derived general functional profiles of the protein coding transcripts. By utilization of domain based functional annotation we identified potential proteases and protease inhibitors and compared their expression in the two types of pollen. Functional profiles are highly similar between Betula verrucosa and Pinus sylvestris pollen. Both pollen contain proteases and inhibitors from 53 and 7 Pfam families, respectively. Some of the members comprised within those families are implicated in facilitating allergen entry, while others are known allergens themselves. Our work revealed several candidate proteins which, with further investigation, represent exciting new leads in elucidating the process behind allergic sensitization. View Full-Text
Keywords: pollen transcriptome; protease; protease inhibitor; allergenicity pollen transcriptome; protease; protease inhibitor; allergenicity

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Höllbacher, B.; Schmitt, A.O.; Hofer, H.; Ferreira, F.; Lackner, P. Identification of Proteases and Protease Inhibitors in Allergenic and Non-Allergenic Pollen. Int. J. Mol. Sci. 2017, 18, 1199.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top