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Mammalian Metallothionein-3: New Functional and Structural Insights
Open AccessArticle

Function of Metallothionein-3 in Neuronal Cells: Do Metal Ions Alter Expression Levels of MT3?

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Department of Chemistry, Barnard College of Columbia University, New York, NY 10027, USA
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Center for Genome Technology and Biomolecular Engineering, Department of Chemical Engineering, Columbia University, New York, NY 10027, USA
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Department of Biology, Barnard College of Columbia University, New York, NY 10027, USA
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Department of Psychology and Program in Neuroscience, Barnard College of Columbia University, New York, NY 10027, USA
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Department of Psychology, Columbia University, New York, NY 10027, USA
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Department of Pathology and Cell Biology Columbia Health Sciences, New York, NY 10027, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Eva Freisinger
Int. J. Mol. Sci. 2017, 18(6), 1133; https://doi.org/10.3390/ijms18061133
Received: 19 April 2017 / Revised: 16 May 2017 / Accepted: 16 May 2017 / Published: 25 May 2017
(This article belongs to the Special Issue Metallothioneins in Bioinorganic Chemistry: Recent Developments)
A study of factors proposed to affect metallothionein-3 (MT3) function was carried out to elucidate the opaque role MT3 plays in human metalloneurochemistry. Gene expression of Mt2 and Mt3 was examined in tissues extracted from the dentate gyrus of mouse brains and in human neuronal cell cultures. The whole-genome gene expression analysis identified significant variations in the mRNA levels of genes associated with zinc homeostasis, including Mt2 and Mt3. Mt3 was found to be the most differentially expressed gene in the identified groups, pointing to the existence of a factor, not yet identified, that differentially controls Mt3 expression. To examine the expression of the human metallothioneins in neurons, mRNA levels of MT3 and MT2 were compared in BE(2)C and SH-SY5Y cell cultures treated with lead, zinc, cobalt, and lithium. MT2 was highly upregulated by Zn2+ in both cell cultures, while MT3 was not affected, and no other metal had an effect on either MT2 or MT3. View Full-Text
Keywords: metallothionein; MT3; metalloneurochemistry; lead neurotoxicity; gene expression; microarrays; dentate gyrus metallothionein; MT3; metalloneurochemistry; lead neurotoxicity; gene expression; microarrays; dentate gyrus
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MDPI and ACS Style

Bousleiman, J.; Pinsky, A.; Ki, S.; Su, A.; Morozova, I.; Kalachikov, S.; Wiqas, A.; Silver, R.; Sever, M.; Austin, R.N. Function of Metallothionein-3 in Neuronal Cells: Do Metal Ions Alter Expression Levels of MT3? Int. J. Mol. Sci. 2017, 18, 1133.

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