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Volume 18
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10.3390/ijms18040834
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Article

Beneficial Effects of Melatonin on the In Vitro Maturation of Sheep Oocytes and Its Relation to Melatonin Receptors

by
Xiuzhi Tian
1,
Feng Wang
1,
Lu Zhang
1,
Changjiu He
1,
Pengyun Ji
1,
Jing Wang
1,
Zhenzhen Zhang
1,
Dongying Lv
1,
Wusiman Abulizi
2,
Xuguang Wang
2,
Zhengxing Lian
1 and
Guoshi Liu
1,*
1
National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2
College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
*
Author to whom correspondence should be addressed.
Academic Editor: Russel J. Reiter
Int. J. Mol. Sci. 2017, 18(4), 834; https://doi.org/10.3390/ijms18040834
Received: 6 March 2017 / Revised: 31 March 2017 / Accepted: 7 April 2017 / Published: 17 April 2017
(This article belongs to the Special Issue Melatonin and Its Analogues: Experimental and Clinical Aspects)
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Abstract

(1) Background: The binding sites of melatonin, as a multifunctional molecule, have been identified in human, porcine, and bovine samples. However, the binding sites and mechanisms of melatonin have not been reported in sheep; (2) Methods: Cumulus–oocyte complexes (COCs) were cultured in TCM-199 supplemented with melatonin at concentrations of 0, 10−3, 10−5, 10−7, 10−9, and 10−11 M. Melatonin receptors (MT1 and MT2) were evaluated via immunofluorescence and Western blot. The effects of melatonin on cumulus cell expansion, nuclear maturation, embryo development, and related gene (GDF9, DNMT1, PTX3, HAS2, and EGFR) expression were investigated. The level of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were evaluated in oocytes and cumulus, respectively; (3) Results: Both MT1 and MT2 were expressed in oocytes, cumulus cells, and granulosa cells. Melatonin with a concentration of 10−7 M significantly enhanced the rates of nuclear maturation, cumulus cells expansion, cleavage, and blastocyst. Melatonin enhanced the expression of BMP15 in oocytes and of PTX3, HAS2, and EGFR in cumulus cells. Melatonin decreased the cAMP level of oocytes but enhanced the cGMP level in oocytes and cumulus cells; (4) Conclusion: The higher presence of MT1 in GV cumulus cells and the beneficial effects of melatonin indicated that its roles in regulating sheep oocyte maturation may be mediated mainly by the MT1 receptor. View Full-Text
Keywords: melatonin; oocyte; meioctic maturation; MT1/MT2; sheep melatonin; oocyte; meioctic maturation; MT1/MT2; sheep
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style

Tian, X.; Wang, F.; Zhang, L.; He, C.; Ji, P.; Wang, J.; Zhang, Z.; Lv, D.; Abulizi, W.; Wang, X.; Lian, Z.; Liu, G. Beneficial Effects of Melatonin on the In Vitro Maturation of Sheep Oocytes and Its Relation to Melatonin Receptors. Int. J. Mol. Sci. 2017, 18, 834. https://doi.org/10.3390/ijms18040834

AMA Style

Tian X, Wang F, Zhang L, He C, Ji P, Wang J, Zhang Z, Lv D, Abulizi W, Wang X, Lian Z, Liu G. Beneficial Effects of Melatonin on the In Vitro Maturation of Sheep Oocytes and Its Relation to Melatonin Receptors. International Journal of Molecular Sciences. 2017; 18(4):834. https://doi.org/10.3390/ijms18040834

Chicago/Turabian Style

Tian, Xiuzhi, Feng Wang, Lu Zhang, Changjiu He, Pengyun Ji, Jing Wang, Zhenzhen Zhang, Dongying Lv, Wusiman Abulizi, Xuguang Wang, Zhengxing Lian, and Guoshi Liu. 2017. "Beneficial Effects of Melatonin on the In Vitro Maturation of Sheep Oocytes and Its Relation to Melatonin Receptors" International Journal of Molecular Sciences 18, no. 4: 834. https://doi.org/10.3390/ijms18040834

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