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Article

Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model

1
College of Pharmacy and Medical Research Center, Chungbuk National University, 194-31 Osongsaengmyeong 1-ro, Osong-eup, Heungduk-gu, Cheongju 361-951, Korea
2
Department of Obstetrics & Gynecology, Daejeon St. Mary’s Hospital, The Catholic University of Korea, 64 Daeheung-Ro (Daeheung-dong), Jung-gu, Daejeon 301-723, Korea
3
Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg 411-15, Sweden
*
Author to whom correspondence should be addressed.
Academic Editor: Allison Cowin
Int. J. Mol. Sci. 2017, 18(4), 738; https://doi.org/10.3390/ijms18040738
Received: 22 January 2017 / Revised: 22 March 2017 / Accepted: 24 March 2017 / Published: 30 March 2017
(This article belongs to the Special Issue Wound Repair and Regeneration)
Centella asiatica has potent antioxidant and anti-inflammatory properties. However, its anti-dermatitic effect has not yet been reported. In this study, we investigated the anti-dermatitic effects of titrated extract of Centella asiatica (TECA) in a phthalic anhydride (PA)-induced atopic dermatitis (AD) animal model as well as in vitro model. An AD-like lesion was induced by the topical application of five percent PA to the dorsal skin or ear of Hos:HR-1 mouse. After AD induction, 100 μL of 0.2% and 0.4% of TECA (40 μg or 80 μg/cm2) was spread on the dorsum of the ear or back skin three times a week for four weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression by Western blotting for inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and NF-κB activity, which were determined by electromobility shift assay (EMSA). We also measured TNF-α, IL-1β, IL-6, and IgE concentration in the blood of AD mice by enzyme-linked immunosorbent assay (ELISA). TECA treatment attenuated the development of PA-induced atopic dermatitis. Histological analysis showed that TECA inhibited hyperkeratosis, mast cells and infiltration of inflammatory cells. TECA treatment inhibited expression of iNOS and COX-2, and NF-κB activity as well as the release of TNF-α, IL-1β, IL-6, and IgE. In addition, TECA (1, 2, 5 μg/mL) potently inhibited Lipopolysaccharide (LPS) (1 μg/mL)-induced NO production, expression of iNOS and COX-2, and NF-κB DNA binding activities in RAW264.7 macrophage cells. Our data demonstrated that TECA could be a promising agent for AD by inhibition of NF-κB signaling. View Full-Text
Keywords: titrated extract of Centella asiatica; skin inflammation; atopic dermatitis; NF-κB; cytokine; IgE titrated extract of Centella asiatica; skin inflammation; atopic dermatitis; NF-κB; cytokine; IgE
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MDPI and ACS Style

Park, J.H.; Choi, J.Y.; Son, D.J.; Park, E.K.; Song, M.J.; Hellström, M.; Hong, J.T. Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model. Int. J. Mol. Sci. 2017, 18, 738. https://doi.org/10.3390/ijms18040738

AMA Style

Park JH, Choi JY, Son DJ, Park EK, Song MJ, Hellström M, Hong JT. Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model. International Journal of Molecular Sciences. 2017; 18(4):738. https://doi.org/10.3390/ijms18040738

Chicago/Turabian Style

Park, Ju H., Ji Y. Choi, Dong J. Son, Eun K. Park, Min J. Song, Mats Hellström, and Jin T. Hong 2017. "Anti-Inflammatory Effect of Titrated Extract of Centella asiatica in Phthalic Anhydride-Induced Allergic Dermatitis Animal Model" International Journal of Molecular Sciences 18, no. 4: 738. https://doi.org/10.3390/ijms18040738

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