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Article

Overexpression and Down-Regulation of Barley Lipoxygenase LOX2.2 Affects Jasmonate-Regulated Genes and Aphid Fecundity

by
Aleksandra Losvik
1,†,
Lisa Beste
1,†,
Robert Glinwood
2,
Emelie Ivarson
3,
Jennifer Stephens
4,
Li-Hua Zhu
3 and
Lisbeth Jonsson
1,*
1
Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden
2
Department of Crop Production Ecology, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden
3
Department of Plant Breeding, Swedish University of Agricultural Sciences, 23053 Alnarp, Sweden
4
Cell and Molecular Science, James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2017, 18(12), 2765; https://doi.org/10.3390/ijms18122765
Submission received: 2 November 2017 / Revised: 8 December 2017 / Accepted: 15 December 2017 / Published: 19 December 2017
(This article belongs to the Special Issue Plant Defense Genes Against Biotic Stresses)
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Abstract

Aphids are pests on many crops and depend on plant phloem sap as their food source. In an attempt to find factors improving plant resistance against aphids, we studied the effects of overexpression and down-regulation of the lipoxygenase gene LOX2.2 in barley (Hordeum vulgare L.) on the performance of two aphid species. A specialist, bird cherry-oat aphid (Rhopalosiphum padi L.) and a generalist, green peach aphid (Myzus persicae Sulzer) were studied. LOX2.2 overexpressing lines showed up-regulation of some other jasmonic acid (JA)-regulated genes, and antisense lines showed down-regulation of such genes. Overexpression or suppression of LOX2.2 did not affect aphid settling or the life span on the plants, but in short term fecundity tests, overexpressing plants supported lower aphid numbers and antisense plants higher aphid numbers. The amounts and composition of released volatile organic compounds did not differ between control and LOX2.2 overexpressing lines. Up-regulation of genes was similar for both aphid species. The results suggest that LOX2.2 plays a role in the activation of JA-mediated responses and indicates the involvement of LOX2.2 in basic defense responses.
Keywords: aphid resistance; lipoxygenase; Hordeum vulgare; Rhopalosiphum padi; Myzus persicae aphid resistance; lipoxygenase; Hordeum vulgare; Rhopalosiphum padi; Myzus persicae
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MDPI and ACS Style

Losvik, A.; Beste, L.; Glinwood, R.; Ivarson, E.; Stephens, J.; Zhu, L.-H.; Jonsson, L. Overexpression and Down-Regulation of Barley Lipoxygenase LOX2.2 Affects Jasmonate-Regulated Genes and Aphid Fecundity. Int. J. Mol. Sci. 2017, 18, 2765. https://doi.org/10.3390/ijms18122765

AMA Style

Losvik A, Beste L, Glinwood R, Ivarson E, Stephens J, Zhu L-H, Jonsson L. Overexpression and Down-Regulation of Barley Lipoxygenase LOX2.2 Affects Jasmonate-Regulated Genes and Aphid Fecundity. International Journal of Molecular Sciences. 2017; 18(12):2765. https://doi.org/10.3390/ijms18122765

Chicago/Turabian Style

Losvik, Aleksandra, Lisa Beste, Robert Glinwood, Emelie Ivarson, Jennifer Stephens, Li-Hua Zhu, and Lisbeth Jonsson. 2017. "Overexpression and Down-Regulation of Barley Lipoxygenase LOX2.2 Affects Jasmonate-Regulated Genes and Aphid Fecundity" International Journal of Molecular Sciences 18, no. 12: 2765. https://doi.org/10.3390/ijms18122765

APA Style

Losvik, A., Beste, L., Glinwood, R., Ivarson, E., Stephens, J., Zhu, L.-H., & Jonsson, L. (2017). Overexpression and Down-Regulation of Barley Lipoxygenase LOX2.2 Affects Jasmonate-Regulated Genes and Aphid Fecundity. International Journal of Molecular Sciences, 18(12), 2765. https://doi.org/10.3390/ijms18122765

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