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Int. J. Mol. Sci. 2017, 18(12), 2750; https://doi.org/10.3390/ijms18122750

Low Levels of IgG Recognizing the α-1-Antitrypsin Peptide and Its Association with Taiwanese Women with Primary Sjögren’s Syndrome

1
Division of Allergy, Immunology, and Rheumatology, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan
2
Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
3
Institute of Labor, Occupational Safety and Health, Ministry of Labor, New Taipei City 23561, Taiwan
4
School of Public Health, National Defense Medical Center, Taipei 11031, Taiwan
5
Graduate Institute of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan
6
School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan
7
Graduate Institute of Data Science, College of Management, Taipei Medical University, Taipei 11031, Taiwan
8
Research Center of Biostatistics, College of Management, Taipei Medical University, Taipei 11031, Taiwan
9
Department of Laboratory Medicine, Taipei Medical University-Shuang-Ho Hospital, Taipei Medical University, New Taipei City 23561, Taiwan
10
Department of Biotechnology and Animal Science, National Ilan University, Ilan 26047, Taiwan
11
Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, Taiwan
*
Author to whom correspondence should be addressed.
Received: 28 October 2017 / Revised: 11 December 2017 / Accepted: 12 December 2017 / Published: 18 December 2017
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Abstract

The aim of this study was to examine oxidative stress and low level of α-1-antitrypsin (A1AT) in primary Sjögren’s syndrome (pSS), and evaluate the associated autoreactivity against unmodified and their 4-hydroxy-2-nonenal (HNE)-modified peptides with pSS. Two differentially expressed proteins, α-1-acid glycoprotein 1 (A1AG1) and A1AT, exhibited 2-fold differences, and their HNE modifications were identified by depleted-albumin and immunoglobulin G (IgG) serum protein, in-solution digestion, in-gel digestion, and nano-liquid chromatography–tandem mass spectrometry (nano-LC-MS/MS) from pSS patients and age-matched healthy controls (HCs). Furthermore, levels of proteins, confirmation of HNE modifications, HNE-protein adducts and autoreactivity against unmodified and their HNE-modified peptides were further validated. Levels of the HNE-protein adduct and A1AG1 were significantly higher in pSS patients than HCs, but levels of A1AT were significantly lower in pSS patients compared to HCs. Only the HNE modification of A1AT was confirmed. Our study suggests that elevated HNE-protein adduct, oxidative stress, level (odds ratio (OR) 4.877, p = 0.003), lowered A1AT level (OR 3.910, p = 0.010) and a decreased level of anti-A1AT50–63 IgG (OR 3.360, p = 0.010) showed an increased risk in pSS patients compared to HCs, respectively. View Full-Text
Keywords: primary Sjögren’s syndrome; α-1-antitrypsin; inhibitor; 4-hydroxy-2-nonenal; autoantibody isotypes; serum primary Sjögren’s syndrome; α-1-antitrypsin; inhibitor; 4-hydroxy-2-nonenal; autoantibody isotypes; serum
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Chang, Y.-S.; Pan, C.-H.; Chang, C.-C.; Tsai, K.-L.; Chou, H.-W.; Chen, J.-H.; Lin, S.-H.; Lu, Y.-Y.; Tai, C.-C.; Lin, Y.-F.; Lin, C.-Y. Low Levels of IgG Recognizing the α-1-Antitrypsin Peptide and Its Association with Taiwanese Women with Primary Sjögren’s Syndrome. Int. J. Mol. Sci. 2017, 18, 2750.

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