Could Oxidative Stress Regulate the Expression of MicroRNA-146a and MicroRNA-34a in Human Osteoarthritic Chondrocyte Cultures?
Abstract
:1. Introduction
2. Results
2.1. Mitochondrial •O2− Production
2.2. Cell Viability Assay
2.3. Apoptosis Detection
2.4. Modifications of Gene Expression Levels of Factors Involved in Oxidant/Antioxidant Systems
2.5. Regulation of miR-146a and miR-34a Gene Expression
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Treatment of Chondrocyte Cultures
4.3. Mitochondrial Superoxide Anion (•O2−) Production
4.4. MTT Assay
4.5. Detection of Apoptotic Cells
4.6. RNA Isolation and RT-qPCR
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Cheleschi, S.; De Palma, A.; Pascarelli, N.A.; Giordano, N.; Galeazzi, M.; Tenti, S.; Fioravanti, A. Could Oxidative Stress Regulate the Expression of MicroRNA-146a and MicroRNA-34a in Human Osteoarthritic Chondrocyte Cultures? Int. J. Mol. Sci. 2017, 18, 2660. https://doi.org/10.3390/ijms18122660
Cheleschi S, De Palma A, Pascarelli NA, Giordano N, Galeazzi M, Tenti S, Fioravanti A. Could Oxidative Stress Regulate the Expression of MicroRNA-146a and MicroRNA-34a in Human Osteoarthritic Chondrocyte Cultures? International Journal of Molecular Sciences. 2017; 18(12):2660. https://doi.org/10.3390/ijms18122660
Chicago/Turabian StyleCheleschi, Sara, Anna De Palma, Nicola Antonio Pascarelli, Nicola Giordano, Mauro Galeazzi, Sara Tenti, and Antonella Fioravanti. 2017. "Could Oxidative Stress Regulate the Expression of MicroRNA-146a and MicroRNA-34a in Human Osteoarthritic Chondrocyte Cultures?" International Journal of Molecular Sciences 18, no. 12: 2660. https://doi.org/10.3390/ijms18122660