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Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures

School of Biological Sciences, University of Reading, Reading RG6 6AS, UK
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(12), 2623;
Received: 30 October 2017 / Revised: 29 November 2017 / Accepted: 30 November 2017 / Published: 5 December 2017
(This article belongs to the Collection Proteins and Protein-Ligand Interactions)
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The elucidation of protein–protein interactions is vital for determining the function and action of quaternary protein structures. Here, we discuss the difficulty and importance of establishing protein quaternary structure and review in vitro and in silico methods for doing so. Determining the interacting partner proteins of predicted protein structures is very time-consuming when using in vitro methods, this can be somewhat alleviated by use of predictive methods. However, developing reliably accurate predictive tools has proved to be difficult. We review the current state of the art in predictive protein interaction software and discuss the problem of scoring and therefore ranking predictions. Current community-based predictive exercises are discussed in relation to the growth of protein interaction prediction as an area within these exercises. We suggest a fusion of experimental and predictive methods that make use of sparse experimental data to determine higher resolution predicted protein interactions as being necessary to drive forward development. View Full-Text
Keywords: protein; interaction; prediction; homology; docking; quality assessment; experimental protein; interaction; prediction; homology; docking; quality assessment; experimental

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Nealon, J.O.; Philomina, L.S.; McGuffin, L.J. Predictive and Experimental Approaches for Elucidating Protein–Protein Interactions and Quaternary Structures. Int. J. Mol. Sci. 2017, 18, 2623.

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