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Int. J. Mol. Sci. 2017, 18(12), 2613;

Every OGT Is Illuminated … by Fluorescent and Synchrotron Lights

DSF-Dipartimento di Scienze del Farmaco, University of Piemonte Orientale, Via Bovio 6, 28100 Novara, Italy
Institute of Biosciences and BioResources, National Research Council of Italy, Via Pietro Castellino 111, 80131 Naples, Italy
Authors to whom correspondence should be addressed.
Received: 15 November 2017 / Revised: 28 November 2017 / Accepted: 30 November 2017 / Published: 5 December 2017
(This article belongs to the Special Issue Chemically-Induced DNA Damage, Mutagenesis, and Cancer)
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O6-DNA-alkyl-guanine-DNA-alkyl-transferases (OGTs) are evolutionarily conserved, unique proteins that repair alkylation lesions in DNA in a single step reaction. Alkylating agents are environmental pollutants as well as by-products of cellular reactions, but are also very effective chemotherapeutic drugs. OGTs are major players in counteracting the effects of such agents, thus their action in turn affects genome integrity, survival of organisms under challenging conditions and response to chemotherapy. Numerous studies on OGTs from eukaryotes, bacteria and archaea have been reported, highlighting amazing features that make OGTs unique proteins in their reaction mechanism as well as post-reaction fate. This review reports recent functional and structural data on two prokaryotic OGTs, from the pathogenic bacterium Mycobacterium tuberculosis and the hyperthermophilic archaeon Sulfolobus solfataricus, respectively. These studies provided insight in the role of OGTs in the biology of these microorganisms, but also important hints useful to understand the general properties of this class of proteins. View Full-Text
Keywords: DNA repair; alkylation damage; conformational changes; protein structure; protein stability; protein-tag DNA repair; alkylation damage; conformational changes; protein structure; protein stability; protein-tag

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Miggiano, R.; Valenti, A.; Rossi, F.; Rizzi, M.; Perugino, G.; Ciaramella, M. Every OGT Is Illuminated … by Fluorescent and Synchrotron Lights. Int. J. Mol. Sci. 2017, 18, 2613.

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