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Article

Real-Time Observation of the Interaction between Thioflavin T and an Amyloid Protein by Using High-Sensitivity Rheo-NMR

1
Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
2
Department of Molecular and Cellular Physiology, Graduate School of Medicine, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
3
Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8552, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2017, 18(11), 2271; https://doi.org/10.3390/ijms18112271
Received: 2 October 2017 / Revised: 25 October 2017 / Accepted: 25 October 2017 / Published: 28 October 2017
(This article belongs to the Collection Proteins and Protein-Ligand Interactions)
Amyloid fibril formation is associated with numerous neurodegenerative diseases. To elucidate the mechanism of fibril formation, the thioflavin T (ThT) fluorescence assay is widely used. ThT is a fluorescent dye that selectively binds to amyloid fibrils and exhibits fluorescence enhancement, which enables quantitative analysis of the fibril formation process. However, the detailed binding mechanism has remained unclear. Here we acquire real-time profiles of fibril formation of superoxide dismutase 1 (SOD1) using high-sensitivity Rheo-NMR spectroscopy and detect weak and strong interactions between ThT and SOD1 fibrils in a time-dependent manner. Real-time information on the interaction between ThT and fibrils will contribute to the understanding of the binding mechanism of ThT to fibrils. In addition, our method provides an alternative way to analyze fibril formation. View Full-Text
Keywords: amyloid fibrils; thioflavin T; molecular interactions; Rheo-NMR; real-time observation; SOD1 amyloid fibrils; thioflavin T; molecular interactions; Rheo-NMR; real-time observation; SOD1
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MDPI and ACS Style

Iwakawa, N.; Morimoto, D.; Walinda, E.; Kawata, Y.; Shirakawa, M.; Sugase, K. Real-Time Observation of the Interaction between Thioflavin T and an Amyloid Protein by Using High-Sensitivity Rheo-NMR. Int. J. Mol. Sci. 2017, 18, 2271. https://doi.org/10.3390/ijms18112271

AMA Style

Iwakawa N, Morimoto D, Walinda E, Kawata Y, Shirakawa M, Sugase K. Real-Time Observation of the Interaction between Thioflavin T and an Amyloid Protein by Using High-Sensitivity Rheo-NMR. International Journal of Molecular Sciences. 2017; 18(11):2271. https://doi.org/10.3390/ijms18112271

Chicago/Turabian Style

Iwakawa, Naoto, Daichi Morimoto, Erik Walinda, Yasushi Kawata, Masahiro Shirakawa, and Kenji Sugase. 2017. "Real-Time Observation of the Interaction between Thioflavin T and an Amyloid Protein by Using High-Sensitivity Rheo-NMR" International Journal of Molecular Sciences 18, no. 11: 2271. https://doi.org/10.3390/ijms18112271

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