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Int. J. Mol. Sci. 2017, 18(12), 2558; https://doi.org/10.3390/ijms18122558

Effect of Dietary Acidolysis-Oxidized Konjac Glucomannan Supplementation on Serum Immune Parameters and Intestinal Immune-Related Gene Expression of Schizothorax prenanti

1,†
,
1,†
,
1
,
1
,
1
and
1,2,*
1
College of Food Science, Sichuan Agricultural University, Yaan 625014, China
2
School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China
These authors contributed equally to the work.
*
Author to whom correspondence should be addressed.
Received: 29 October 2017 / Revised: 22 November 2017 / Accepted: 26 November 2017 / Published: 28 November 2017
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [1066 KB, uploaded 29 November 2017]   |  

Abstract

The present study was conducted to investigate the effects of dietary acidolysis-oxidized konjac glucomannan (A-OKGM) (0%, 0.4%, 0.8%, and 1.6%) supplementation on the immunity and expression of immune-related genes in Schizothorax prenanti. After feeding for eight weeks, the serum and guts were used for measurement of biochemical parameters, and immune-related gene expression in the gut were also analyzed by real-time quantitative polymerase chain reaction (RT-qPCR). C-reactive protein and IgM levels were significantly higher in the A-OKGM fed groups than in the control group, regardless of the dosage. The 0.4% and 1.6% A-OKGM groups showed significant up-regulation of tumor necrosis factor α (TNFα) in the anterior gut. The 0.8% and 1.6% A-OKGM groups also showed significantly enhanced TNFα expression in the mid- and distal guts. Interleukin-1β (IL-1β) expression in the anterior gut of fish fed with 0.4% and 1.6% A-OKGM diets was significantly enhanced. The 0.8% and 1.6% A-OKGM diets resulted in significantly increased the expression of IL-1β in the distal gut. Similarly, the interleukin-6 (IL-6) messenger RNA (mRNA) levels in the 0.4% and 1.6% diet groups were significantly higher in the anterior gut. The 0.8% and 1.6% A-OKGM diet groups showed significant induction of IL-6 gene expression in the distal gut. A-OKGM modified from KGM can act as an immunostimulant to enhance the immunity of S. prenanti. View Full-Text
Keywords: Schizothorax prenanti; acidolysis-oxidized konjac glucomannan; immunity Schizothorax prenanti; acidolysis-oxidized konjac glucomannan; immunity
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Chen, M.; Wang, S.; Liang, X.; Ma, D.; He, L.; Liu, Y. Effect of Dietary Acidolysis-Oxidized Konjac Glucomannan Supplementation on Serum Immune Parameters and Intestinal Immune-Related Gene Expression of Schizothorax prenanti. Int. J. Mol. Sci. 2017, 18, 2558.

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