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Development and Sequential Analysis of a New Multi-Agent, Anti-Acne Formulation Based on Plant-Derived Antimicrobial and Anti-Inflammatory Compounds

Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Faculty of Biology, SplaiulIndependenţei 91-95 Sector 5, 76201 Bucharest, Romania
S.C. SANIMED INTERNATIONAL IMPEX S.R.L., Sos. Bucuresti-Magurele, nr.70, Sector 5, 051434 Bucharest, Romania
S.C. Biotehnos S.A., Gorunului Str. 3-5, 075100 Otopeni, Ilfov, Romania
Ştefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, Sector 3, 030304 Bucharest, Romania
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Chris Jackson
Int. J. Mol. Sci. 2017, 18(1), 175;
Received: 30 October 2016 / Revised: 5 January 2017 / Accepted: 10 January 2017 / Published: 17 January 2017
(This article belongs to the Special Issue Inflammatory Skin Conditions)
PDF [1151 KB, uploaded 17 January 2017]


The antibacterial and anti-inflammatory potential of natural, plant-derived compounds has been reported in many studies. Emerging evidence indicates that plant-derived essential oils and/or their major compounds may represent a plausible alternative treatment for acne, a prevalent skin disorder in both adolescent and adult populations. Therefore, the purpose of this study was to develop and subsequently analyze the antimicrobial activity of a new multi-agent, synergic formulation based on plant-derived antimicrobial compounds (i.e., eugenol, β-pinene, eucalyptol, and limonene) and anti-inflammatory agents for potential use in the topical treatment of acne and other skin infections. The optimal antimicrobial combinations selected in this study were eugenol/β-pinene/salicylic acid and eugenol/β-pinene/2-phenoxyethanol/potassium sorbate. The possible mechanisms of action revealed by flow cytometry were cellular permeabilization and inhibition of efflux pumps activity induced by concentrations corresponding to sub-minimal inhibitory (sub-MIC) values. The most active antimicrobial combination represented by salycilic acid/eugenol/β-pinene/2-phenoxyethanol/potassium sorbate was included in a cream base, which demonstrated thermodynamic stability and optimum microbiological characteristics. View Full-Text
Keywords: plant-derived compounds; antimicrobial; acne; eugenol; β-pinene; eucalyptol; limonene plant-derived compounds; antimicrobial; acne; eugenol; β-pinene; eucalyptol; limonene

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Saviuc, C.; Ciubucă, B.; Dincă, G.; Bleotu, C.; Drumea, V.; Chifiriuc, M.-C.; Popa, M.; Pircalabioru, G.G.; Marutescu, L.; Lazăr, V. Development and Sequential Analysis of a New Multi-Agent, Anti-Acne Formulation Based on Plant-Derived Antimicrobial and Anti-Inflammatory Compounds. Int. J. Mol. Sci. 2017, 18, 175.

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