Int. J. Mol. Sci. 2016, 17(9), 1527; https://doi.org/10.3390/ijms17091527
Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea
Jun-Jie Yan 1,†
, Bin Xie 1,†, Lei Zhang 1, Shao-Jie Li 2, Arend F. van Peer 1, Ta-Ju Wu 2, Bing-Zhi Chen 1 and Bao-Gui Xie 1,*
, Bin Xie 1,†, Lei Zhang 1, Shao-Jie Li 2, Arend F. van Peer 1, Ta-Ju Wu 2, Bing-Zhi Chen 1 and Bao-Gui Xie 1,*
1
Mycological Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jianhua Zhu
Received: 30 June 2016 / Revised: 5 September 2016 / Accepted: 7 September 2016 / Published: 10 September 2016
(This article belongs to the Section Biochemistry)
Abstract
Small GTPases play important roles in the growth, development and environmental responses of eukaryotes. Based on the genomic sequence of the straw mushroom Volvariella volvacea, 44 small GTPases were identified. A clustering analysis using human small GTPases as the references revealed that V. volvacea small GTPases can be grouped into five families: nine are in the Ras family, 10 are in the Rho family, 15 are in the Rab family, one is in the Ran family and nine are in the Arf family. The transcription of vvran1 was up-regulated upon hydrogen peroxide (H2O2) stress, and could be repressed by diphenyleneiodonium chloride (DPI), a NADPH oxidase-specific inhibitor. The number of vvran1 transcripts also increased upon cold stress. Diphenyleneiodonium chloride, but not the superoxide dismutase (SOD) inhibitor diethy dithiocarbamate (DDC), could suppress the up-regulation of vvran1 gene expression to cold stress. These results combined with the high correlations between gene expression and superoxide anion (O2−) generation indicated that vvran1 could be one of the candidate genes in the downstream of O2− mediated pathways that are generated by NADPH oxidase under low temperature and oxidative stresses. View Full-Text
Graphical abstract
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Yan, J.-J.; Xie, B.; Zhang, L.; Li, S.-J.; van Peer, A.F.; Wu, T.-J.; Chen, B.-Z.; Xie, B.-G. Small GTPases and Stress Responses of vvran1 in the Straw Mushroom Volvariella volvacea. Int. J. Mol. Sci. 2016, 17, 1527.
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