Next Article in Journal
Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster
Previous Article in Journal
Impact of Prematurity and Perinatal Antibiotics on the Developing Intestinal Microbiota: A Functional Inference Study
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 17
Issue 5
10.3390/ijms17050628
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Mycoplasma bovis MBOV_RS02825 Encodes a Secretory Nuclease Associated with Cytotoxicity

by
Hui Zhang
1,2,
Gang Zhao
1,2,
Yusi Guo
1,2,
Harish Menghwar
1,2,
Yingyu Chen
1,2,3,4,
Huanchun Chen
1,2,3,4 and
Aizhen Guo
1,2,3,4,*
1
The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
2
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
3
Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China
4
Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2016, 17(5), 628; https://doi.org/10.3390/ijms17050628
Submission received: 22 February 2016 / Revised: 13 April 2016 / Accepted: 15 April 2016 / Published: 29 April 2016
(This article belongs to the Section Biochemistry)
Browse Figures
Versions Notes

Abstract

This study aimed to determine the activity of one Mycoplasma bovis nuclease encoded by MBOV_RS02825 and its association with cytotoxicity. The bioinformatics analysis predicted that it encodes a Ca2+-dependent nuclease based on existence of enzymatic sites in a TNASE_3 domain derived from a Staphylococcus aureus thermonuclease (SNc). We cloned and purified the recombinant MbovNase (rMbovNase), and demonstrated its nuclease activity by digesting bovine macrophage linear DNA and RNA, and closed circular plasmid DNA in the presence of 10 mM Ca2+ at 22–65 °C. In addition, this MbovNase was localized in membrane and rMbovNase able to degrade DNA matrix of neutrophil extracellular traps (NETs). When incubated with macrophages, rMbovNase bound to and invaded the cells localizing to both the cytoplasm and nuclei. These cells experienced apoptosis and the viability was significantly reduced. The apoptosis was confirmed by activated expression of phosphorylated NF-κB p65 and Bax, and inhibition of Iκβα and Bcl-2. In contrast, rMbovNaseΔ181–342 without TNASE_3 domain exhibited deficiency in all the biological functions. Furthermore, rMbovNase was also demonstrated to be secreted. In conclusion, it is a first report that MbovNase is an active nuclease, both secretory and membrane protein with ability to degrade NETs and induce apoptosis.
Keywords: MBOV_RS02825; Mycoplasma bovis; nuclease; secretory protein; TNASE_3 domain; neutrophil extracellular traps (NETs); apoptosis; binding and internalization MBOV_RS02825; Mycoplasma bovis; nuclease; secretory protein; TNASE_3 domain; neutrophil extracellular traps (NETs); apoptosis; binding and internalization
Graphical Abstract

Share and Cite

MDPI and ACS Style

Zhang, H.; Zhao, G.; Guo, Y.; Menghwar, H.; Chen, Y.; Chen, H.; Guo, A. Mycoplasma bovis MBOV_RS02825 Encodes a Secretory Nuclease Associated with Cytotoxicity. Int. J. Mol. Sci. 2016, 17, 628. https://doi.org/10.3390/ijms17050628

AMA Style

Zhang H, Zhao G, Guo Y, Menghwar H, Chen Y, Chen H, Guo A. Mycoplasma bovis MBOV_RS02825 Encodes a Secretory Nuclease Associated with Cytotoxicity. International Journal of Molecular Sciences. 2016; 17(5):628. https://doi.org/10.3390/ijms17050628

Chicago/Turabian Style

Zhang, Hui, Gang Zhao, Yusi Guo, Harish Menghwar, Yingyu Chen, Huanchun Chen, and Aizhen Guo. 2016. "Mycoplasma bovis MBOV_RS02825 Encodes a Secretory Nuclease Associated with Cytotoxicity" International Journal of Molecular Sciences 17, no. 5: 628. https://doi.org/10.3390/ijms17050628

APA Style

Zhang, H., Zhao, G., Guo, Y., Menghwar, H., Chen, Y., Chen, H., & Guo, A. (2016). Mycoplasma bovis MBOV_RS02825 Encodes a Secretory Nuclease Associated with Cytotoxicity. International Journal of Molecular Sciences, 17(5), 628. https://doi.org/10.3390/ijms17050628

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop