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Int. J. Mol. Sci. 2016, 17(12), 2134;

Recent Methods for Purification and Structure Determination of Oligonucleotides

1,2,3,4,* , 1,3,4,* and 1,3,4,*
Institute of Integrated Bioinformedicine and Translational Science, School of Chinese Medicine, Hong Kong Baptist University (HKBU), Hong Kong, China
The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China
Institute of Precision Medicine and Innovative Drug Discovery, HKBU (Haimen) Institute of Science and Technology, Haimen 226100, China
Shenzhen Lab of Combinatorial Compounds and Targeted Drug Delivery, HKBU Institute of Research and Continuing Education, Shenzhen 518000, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Mateus Webba da Silva
Received: 25 November 2016 / Revised: 13 December 2016 / Accepted: 14 December 2016 / Published: 18 December 2016
(This article belongs to the Special Issue Translational Molecular Medicine & Molecular Drug Discovery)
Full-Text   |   PDF [570 KB, uploaded 21 December 2016]   |  


Aptamers are single-stranded DNA or RNA oligonucleotides that can interact with target molecules through specific three-dimensional structures. The excellent features, such as high specificity and affinity for target proteins, small size, chemical stability, low immunogenicity, facile chemical synthesis, versatility in structural design and engineering, and accessible for site-specific modifications with functional moieties, make aptamers attractive molecules in the fields of clinical diagnostics and biopharmaceutical therapeutics. However, difficulties in purification and structural identification of aptamers remain a major impediment to their broad clinical application. In this mini-review, we present the recently attractive developments regarding the purification and identification of aptamers. We also discuss the advantages, limitations, and prospects for the major methods applied in purifying and identifying aptamers, which could facilitate the application of aptamers. View Full-Text
Keywords: aptamers; purification; identification aptamers; purification; identification

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Zhang, Q.; Lv, H.; Wang, L.; Chen, M.; Li, F.; Liang, C.; Yu, Y.; Jiang, F.; Lu, A.; Zhang, G. Recent Methods for Purification and Structure Determination of Oligonucleotides. Int. J. Mol. Sci. 2016, 17, 2134.

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