Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca2+, CaM and Depolarization of Plasma Membrane Potential
1
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
2
School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
3
College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095 China
4
School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Hatem Rouached
Int. J. Mol. Sci. 2016, 17(1), 57; https://doi.org/10.3390/ijms17010057
Received: 4 December 2015 / Revised: 20 December 2015 / Accepted: 22 December 2015 / Published: 5 January 2016
(This article belongs to the Special Issue Plant Molecular Biology)
Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca2+ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca2+ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca2+ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca2+ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca2+ and H+ efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca2+-CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants.
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Keywords:
Ca2+ efflux; Ca2+ fluorescence; CaM; fluoride; NPPB; tea plant
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MDPI and ACS Style
Zhang, X.-C.; Gao, H.-J.; Yang, T.-Y.; Wu, H.-H.; Wang, Y.-M.; Zhang, Z.-Z.; Wan, X.-C. Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca2+, CaM and Depolarization of Plasma Membrane Potential. Int. J. Mol. Sci. 2016, 17, 57.
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