Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca2+, CaM and Depolarization of Plasma Membrane Potential

Zhang, Xian-Chen; Gao, Hong-Jian; Yang, Tian-Yuan; Wu, Hong-Hong; Wang, Yu-Mei; Zhang, Zheng-Zhu; Wan, Xiao-Chun

doi:10.3390/ijms17010057

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Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca²⁺, CaM and Depolarization of Plasma Membrane Potential

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State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China

School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095 China

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School of Land and Food, University of Tasmania, Hobart, Tasmania 7001, Australia

Author to whom correspondence should be addressed.

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These authors contributed equally to this work.

Academic Editor: Hatem Rouached

Int. J. Mol. Sci. 2016, 17(1), 57; https://doi.org/10.3390/ijms17010057

Received: 4 December 2015 / Revised: 20 December 2015 / Accepted: 22 December 2015 / Published: 5 January 2016

(This article belongs to the Special Issue Plant Molecular Biology)

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Abstract

Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca²⁺ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca²⁺ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca²⁺ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca²⁺ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca²⁺ and H⁺ efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca²⁺-CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants. View Full-Text

Keywords: Ca²⁺ efflux; Ca²⁺ fluorescence; CaM; fluoride; NPPB; tea plant

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Zhang, X.-C.; Gao, H.-J.; Yang, T.-Y.; Wu, H.-H.; Wang, Y.-M.; Zhang, Z.-Z.; Wan, X.-C. Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca²⁺, CaM and Depolarization of Plasma Membrane Potential. Int. J. Mol. Sci. 2016, 17, 57. https://doi.org/10.3390/ijms17010057

AMA Style

Zhang X-C, Gao H-J, Yang T-Y, Wu H-H, Wang Y-M, Zhang Z-Z, Wan X-C. Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca²⁺, CaM and Depolarization of Plasma Membrane Potential. International Journal of Molecular Sciences. 2016; 17(1):57. https://doi.org/10.3390/ijms17010057

Chicago/Turabian Style

Zhang, Xian-Chen, Hong-Jian Gao, Tian-Yuan Yang, Hong-Hong Wu, Yu-Mei Wang, Zheng-Zhu Zhang, and Xiao-Chun Wan. 2016. "Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca²⁺, CaM and Depolarization of Plasma Membrane Potential" International Journal of Molecular Sciences 17, no. 1: 57. https://doi.org/10.3390/ijms17010057

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Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca²⁺, CaM and Depolarization of Plasma Membrane Potential

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